The influence of fertilization with fresh sewage sludge with the addition of calcium oxide and lignite ash in the proportions dry mass 6:1, 4:1, 3:1 and 2:1 on the content of chromium and copper in plants and soil and uptake of these elements was investigated in pot experiment. Sewage sludge were taken from Siedlce (sludge after methane fermentation) and Łuków (sludge stabilized in oxygenic conditions), eastern Poland. The chromium content in the biomass of the test plants (maize, sunflower and oat) was higher following the application of mixtures of sewage sludge with ash than of the mixtures with CaO. The copper content in plants most often did not significantly depend on the type of additives to the sludge. Various amounts of additives to the sewage sludge did not have a significant effect on the contents of either of the studied trace elements in plants. The contents of chromium and copper in soil after 3 years of cultivation of plants were higher than before the experiment, but these amounts were not significantly differentiated depending on the type and the amount of the used additive (i.e. CaO vs. ash) to sewage sludge.