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  • Author: Yu Ma x
  • Food Science and Technology x
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Effect of Microencapsulation on Survival and Stability of Bifidobacterium bifidum BB01 Exposed to Simulated Gastrointestinal Conditions and in Different Food Matrices

Abstract

Bifidobacterium bifidum BB01 was microencapsulated by extrusion method with sodium alginate. This paper aimed to study the survival rate of microencapsulating Bifidobacterium bifidum BB01 in simulated gastrointestinal conditions, resistance to artificial bile salt and stability during storage. Results showed non-microencapsulated Bifidobacterium bifidum BB01 were more susceptible than microencapsulated under simulated gastrointestinal conditions, and microencapsulated Bifidobacterium bifidum BB01 exhibited a lower population reduction than free cells during exposure to simulated gastrointestinal, The enteric test showed that the microorganism cells were released from the monolayer microcapsules, double microcapsules and trilayer completely in 40min. The viable counts of monolayer microcapsules, double layer microcapsules and triple layer microcapsules decreased by nine magnitudes, four magnitude and one magnitude after 2h, respectively. Moreover, in fruit orange, pure milk and nutrition Express, the optimum storage times of free Bifidobacterium bifidum BB01, monolayer microcapsules, double layer microcapsules and triple layer microcapsules were 21 days, 21 days, 28 days and more than 35 days at 4°C, but at room temperature the optimum storage time were 7 days, 14 days, 21 days and more than 28 days, and the viable counts were maintained at 1×106 CFU g−1 or more.

Open access
Optimization the Process of Microencapsulation of Bifidobacterium bifidum BB01 by Box-Behnken Design

Abstract

The effects of cell suspension-alginate ratios, sodium erythorbate, and inulin on encapsulation yield of microcapsules of Bifidobacterium bifidum BB01 were studied by Box-Behnken design. The experimental results indicated that cell suspension-alginate ratios, sodium erythorbate and inulin had a significant impact on encapsulation yield, and the embedding yield could be enhanced significantly in the condition of 1:3 cell suspension-alginate ratios, 0.12% sodium erythorbate, and 6% inulin. The optimal embedding yields of microencapsulation of B. bifidum BB01 were observed to be 81.52%, that values were very close to the expected values 81.81%, so the method was effective.

Open access