Tomoki Hattori, Yang Chen, Shinichi Enoki, Daisuke Igarashi and Shunji Suzuki
Berry skin colour is a crucial determinant of red/black grape berry quality. We investigated the effects of combination treatments with amino acids and a low concentration of ABA on anthocyanin accumulation in grapes. Among the amino acids tested, isoleucine and phenylalanine resulted in high anthocyanin contents in grape cell cultures. The combination treatments with isoleucine or phenylalanine, and a low concentration of ABA enhanced anthocyanin accumulation in grape cells and detached grape berries. The combination treatment with isoleucine, but not with phenylalanine, and ABA upregulated MybA1 expression. Field-grown grapevines received combination treatments with isoleucine or phenylalanine, and ABA in two growing seasons. In the 2015 growing season, the combination treatments with isoleucine or phenylalanine, and a low concentration of ABA accelerated anthocyanin accumulation in grape berry skins of field-grown grapevines on days 10 and 31 post treatment. The effects on anthocyanin accumulation became negligible at harvest. The effect of the combination treatment with phenylalanine and a low concentration of ABA on anthocyanin accumulation was masked in the 2017 growing season due to the unexpected stimulation of anthocyanin accumulation by the low concentration of ABA, although the combination treatment accelerated anthocyanin accumulation on days 3 and 10 post treatment. Taken together, the results suggested that exogenous isoleucine and phenylalanine interacted with ABA-mediated anthocyanin accumulation in grape berry skins of field-grown grapevines when the activity of ABA used to treat the grapevines was inadequate.
Xu Chen, Xiangdong Yang, Xuhui Dong and Enfeng Liu
- The spatial distribution of surface sediment diatoms in Chaohu Lake (southeast China), and their relationships with environmental and spatial variables were analyzed in this study. The diatom assemblages were dominated by planktonic species. Three dominant species Cyclostephanos dubius, Aulacoseira granulata and Aulacoseira alpigena are unevenly distributed across the lake. The distribution of surface sediment diatoms must be subject to trophic status, hydrodynamics and other spatial variables in the lake.
After China’s collective forest tenure reform, cooperation organization has played an important role on the development of community forestry. In order to analyse the different stakeholders’ opinion and suggestion on the development of forestry cooperation organization, publicizing and training, participatory interview, brainstorming, and questionnaire surveys were used in this case study. According to the results it can be seen that the real motivation for the development of cooperatives is farmers’ demands and the demands come from farmers’ pursuit of interests in forest industry. Cooperatives in China are still in the early stage of development, and the cooperative laws cannot cover all the basic features of the forestry and the cooperatives. Therefore, the relevant laws and guarantee systems for resource exploitation and management, publicity and demonstration, forestry technology training as well as the preferential policies such as tax breaks, are needed for the sound development of China’s forestry cooperation organization.
Weixing Li, Zhichong He, Shunbo Yang, Yunling Ye, Huiru Jiang and Li Wang
To gain insights into the regulatory networks of miRNAs related to golden colour formation in Ginkgo biloba leaves, we constructed an sRNA library of golden-green striped mutant leaves. A total of 213 known miRNAs comprising 54 miRNA families were obtained, and 214 novel miRNAs were identified in the mutant leaves. We further constructed a normal green leaf sRNA library as a control and compared the expression of miRNAs between mutant and normal leaves. We found 42 known and 54 novel differential expression candidate miRNAs; 39 were up-regulated and 57 down-regulated in mutants compared to normal leaves. Our transcriptome analysis and annotation of the predicted targets indicated that the potential roles of miRNAs in G. biloba leaves included involvement in the ‘Glutathione metabolism’, ‘Plant circadian rhythm’, and ‘Phenylalanine metabolism’ categories. miRNAs and their targets were further validated by qRT-PCR. The expression of miR159a and miR159c, in particular, was significantly higher in mutant leaves than in normal leaves, while their potential target gene CLT3, which is associated with chloroplast development, displayed the opposite expression pattern. In addition, the expression of miR396g-3p and miR396h was also significantly higher in mutant leaves than in normal leaves, while the target genes ABP1 (auxin-related gene) and PPR32 (chloroplast RNA editing protein), respectively, showed the opposite expression pattern. Combined with the transcriptome analysis, these data suggest that miR159, miR396, and their targets may participate in chloroplast development and hormone metabolism to regulate colour formation in G. biloba leaves.