Search Results

1 - 1 of 1 items :

  • Author: Magdalena Kosińska x
  • Biotechnology x
Clear All Modify Search
SE-HPLC-DAD Analysis of Flaxseed Lignan Macromolecule and its Hydrolysates

SE-HPLC-DAD Analysis of Flaxseed Lignan Macromolecule and its Hydrolysates

A lignan macromolecule (LM) was extracted from defatted flaxseeds using an ethanol-dioxan system (1:1, v/v) and purified using Amberlite column chromatography with water and methanol as mobile phases. The LM was subjected to chemical hydrolysis (base, acid, base & acid), as well as to enzymatic processing using pepsin, pancreatin, cellulase, and β-glucuronidase.

The study revealed that lignan macromolecule in flaxseed was not homogenous. The chemical hydrolysis as well as enzymatic treatment using β-glucuronidase and cellulase released low molecular phenolic compounds from the lignan macromolecule. The liberation of secoisolariciresinol (SECO) and free phenolic acids (p-coumaric and ferulic acids) from flaxseed lignan macromolecule as a result of the base and acid hydrolyses was noted. The application of pepsin and pancreatin did not change the composition of the lignan macromolecule.

Open access