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  • Author: Li-Chun Zhang x
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Open access

Yong Guo, Xizheng Zhang, Chunqiu Zhang, Ruixin Li, Qiangcheng Zeng, Chun Guo and Yanjun Zhang

Abstract

Background: Mesenchymal stem cells (MSCs) known to be sensitive to mechanical stimulus. This type of stimulus plays a role in cellular differentiation, so that it might affect MSCs differentiation toward cardiomyocytes.

Objectives: Investigate the effect of mechanical stimulus on MSCs differentiation toward cardiomyocytes.

Methods: The adipose tissue-derived MSCs were induced to differentiate with 5-azacytidine, and stimulated by one Hz mechanical stretching up to 8%. After 10 days, the cell’s cardiac markers and cardiogenesis-related genes were detected by immumohistochemistrical staining and reverse transcriptase-polymerase chain reaction, and the cell’s ATPase activity was detected.

Results: The cyclic mechanical stretching enhanced the expression of cardiogenesis-related genes and cardiac markers, and stimulated the activity of Na+-K+-ATPase and Ca2+-ATPase in the MSCs treated with 5-azacytidine. Without 5-azacytidine pre-treatment, cyclic mechanical stretch alone has little effect.

Conclusion: Mechanical stretch combined with 5-azacytidine treatment could accelerate MSCs differentiation toward cardiomyocytes.

Open access

Zhang Zhi-yong, Li Ming-xu, Yu Yong-wu and Zhou Chun-hua

Abstract

Background: At present, patients with end-stage renal failure (ESRD) face the question of how to achieve adequate dialysis to maintain their survival with the best quality of life as long as possible. Therefore, the choice of suitable dialysis methods and dialysis dose is important.

Objective: To find suitable dialysis methods and an appropriate dialysis dose for patients with ESRD.

Methods: Our research compares the long-term clinical effects between the patients undergoing continuous ambulatory peritoneal dialysis (CAPD), daytime ambulatory peritoneal dialysis (DAPD), and hemodialysis (HD). Thirty-two patients subjected to peritoneal dialysis were selected and divided into group A (n = 16) and group B (n = 16), group A was treated by using the traditional CAPD pattern; group B was treated by using DAPD. Forty-six patients of renal failure at final stage were divided into group A (n = 23) and group B (n = 23), group A was treated by using a HD method, group B was treated by using a DAPD method.

Results: When the same dialysis dose was applied, the patients with long-term DAPD treatment can obtain satisfactory clinical therapeutic efficacy for the period of maintenance dialysis, thoroughness of dialysis, maintenance of nutritional status, blood pressure regulation, reduction in incidence rate of peritoneal infection, maintenance of relatively high activity in daily life and alleviation in depression, comparable to patients treated with traditional CAPD or HD methods.

Conclusion: The long-term clinical effects of DAPD are comparable to CAPD or HD.

Open access

Yue-qiu Zhang, Shao-xia Xu, Sai-nan Bian, Li-fan Zhang, Yao Zhang, Wei-hong Zhang, Ying-chun Xu and Xiao-qing Liu

Abstract

Objective To investigate the infection rate of hepatitis C virus among the ambulatory patients and in-patients of a tertiary teaching hospital, and study the demographic factors related to the prevalence of hepatitis C virus infection.

Methods All patients tested for hepatitis C virus antibody from July 2008 to July 2009 in Peking Union Medical College Hospital were enrolled in this cross-sectional analysis. The prevalence of hepatitis C virus infection was compared according to age, gender, and departments, respectively. Among patients with positive serology hepatitis C virus marker, the positivity of hepatitis C virus RNA was analysed.

Results Among 29 896 subjects included, the hepatitis C virus antibody of 494 patients were positive (1.7%). When patients were divided into 9 age groups, the age specific prevalence of hepatitis C virus antibody were 0.2%, 1.7%, 1.2%, 1.1%, 1.5%, 1.9%,2.6%, 2.4% and 2%, respectively. The prevalence of hepatitis C virus antibody in non-surgical department and surgical department was 3% and 1%, respectively. The prevalence of hepatitis C virus antibody of males was higher than that of the females. Total of 194 patients with positive hepatitis C virus antibody were tested for hepatitis C virus RNA, the RNA level of 113 patients (58.2%) were higher than the low detection limit.

Conclusions The prevalence of hepatitis C virus antibody was relatively high among patients of general tertiary hospital. Age group of 60-69, males and patients in non-surgical departments were factors associated with high rate of hepatitis C virus infection.

Open access

Pei-yan Zhang, Si-min Yao, Fan Zhang, Ming-xia Zhang, Wei Li, Jing Yuan, Xin-chun Chen and Bo-ping Zhou

Abstract

Objective Various immune cells in patients with CHB have been demonstrated to play critical roles in HBV infection. The goal of this study is to observe changes in Th17, Treg, Th1 and B lymphocytes from peripheral blood and to evaluate immune status of CHB patients undergoing antiviral treatment.

Methods Total of 49 CHB patients, 19 asymptomatic carriers and 29 healthy donors were included in our present study. The frequencies of peripheral Th17 cells (CD3+CD4+IL-17+Tcells), Treg cells (CD3+CD4+CD25+CD127- T cells), Th1 cells (CD3+CD4+IFN-γ T cells) and B lymphocytes in chronic hepatitis B (CHB) were analyzed by flow cytometry.

Results The frequency of Th17 cells increased after treatment for 6 months, but there was no statistically significant difference of IL-17 expression between baseline and 6 months after treatment. The frequencies of Treg cells, momory B cells and total CD19+ B cells decreased after antiviral treatment. The frequencies of Th1 cells and plasma cells increased after antiviral treatment.

Conclusions This study highlights that the reestablishment of immune function during antiviral treatment in CHB patients, which caused by the antiviral drugs or the patients themselves. CHB patients may exhibit varied responses to these antiviral drugs. It is essential to supplement immune therapy during the antiviral treatment, but Th17 may play a limited role in inflammation during antiviral treatment, targeting Th17 therapy may not be useful for CHB treatment. More time and more experiments are critical to explain it.

Open access

Chun-ling Liu, Chun-wei Wang, Ying Liu, Ping Zhou, Xiao-juan Li, Hui-yuan Si and Hongwei Zhang

Abstract

A twenty-eight-year-old male patient with five-day’s fever (the highest body tempreture reached 39.4°C) and 10-hour’s rash (first on face) presented to the department of emergency for “drug rash”, at that time his temperature was 38.6°C. Two hours later, his temperature fell to normal. Then this patient’s entire body rash increased significantly and lasted for 13 hours. Serum measles antibody IgM(+) confirmed the measles diagnosis. He had received measles vaccine as a baby. Clinicians should be aware of this atypical clinical manifestation of adult measles. If this kind of patients were misdiagnosed as drug rash and given corticosteroid, measles disease may be aggravated. Specific serum measles antibody testing may be the only reliable method for differential diagnosis, but the earliest time point for examining the antibodies of measles still needs precise research.

Open access

Gui-lin Yang, Ying-xia Liu, Mu-tong Fang, Wei-long Liu, Xin-chun Chen, John Nunnari, Jing-jing Xie, Ming-feng Liao, Ming-xia Zhang, Guo-bao Li, Pei-ze Zhang, Yi Guan and Boping Zhou

Abstract

Objective To explore whether age, disease severity, cytokines and lymphocytes in H1N1 influenza A patients correlate with viral load and clearance.

Methods Total of 70 mild and 16 severe patients infected with H1N1 influenza A virus were enrolled in this study.

Results It was found that the patients under 14 years old and severe patients displayed significantly higher viral loads and prolonged viral shedding periods compared with the patients over 14 years old and mild patients, respectively (P < 0.05). Moreover, the patients under 14 years old and severe patients displayed significantly lower Th17 cell frequency than the patients over 14 years old and mild patients (P < 0.01). The viral shedding period inversely correlated with the frequency of IL-17+IFN-γ-CD4+ T cells. Additionally, the decreased concentration of serum TGF-β correlated with the decreased frequency of IL-17+IFN-γ-CD4+ T cells.

Conclusions Both younger and severe patients are associated with higher viral loads and longer viral shedding periods, which may partially be attributed to the impaired Th17 cell response.