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  • Author: Li Gao x
  • Microbiology, Virology and Infection Epidemiology x
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Open access

Qin Zhou, Yu-feng Gao, Xiao-miao Zhao, Fa-ming Pan and Xu Li

Abstract

Objective To investigate the relationship between single nucleotide polymorphisms (SNPs) of the interleukin-4 (IL-4) gene and outcome of hepatitis B virus (HBV) infection in a Chinese Han population.

Methods Total of 501 patients with chronic hepatitis B virus (HBV) infection and 301 controls with selflimiting HBV infection were studied. Three tag SNPs in the IL-4 gene (rs2227284G/T, rs2243283C/G and rs2243288A/G) were genotyped by the Multiplex snapshot technique. The genotype and allele frequencies were calculated and analyzed.

Results The three SNPs showed no significant genotype/allele associations with chronic HBV infection. Overall allele P values were: rs2227284, P = 0.655, odds ratio (OR) [95% confidence interval (CI)] = 1.070 (0.793-1.445); rs2243283, P = 0.849, OR (95% CI) = 0.976 (0.758-1.257); rs2243288, P = 0.659, OR (95% CI) = 1.060 (0.818-1.375). Overall genotype P values were: rs2227284, P = 0.771; rs2243283, P = 0.571; rs2243288, P = 0.902. There were no statistically significant differences between patients with chronic HBV infection and controls. Haplotypes generated by these three SNPs also had no significant differences between the two groups.

Conclusions The three tag SNPs of IL-4 were not associated with the outcome of HBV infection in the Han Chinese population.

Open access

Jun Cheng, Min Li, Ping Gao, Jin-ling Dong and Qi Wang

Abstract

Liver steatosis is a pathological hallmark in patients with chronic hepatitis C (CHC). Increased lipid uptake, decreased lipid secretion, increased lipid synthesis and decreased lipid degradation are all involved in pathogenesis of steatosis induced by hepatitic C virus (HCV) infection. Level of low density lipoprotein receptor (LDL-R) and activity of peroxisome proliferator-activated receptor (PPAR) α is related to liver uptake of lipid from circulation, and affected by HCV. Secretion via microsomal triglyceride transfer protein (MTTP), and formation of very low density lipoprotein (VLDL) have been hampered by HCV infection. Up-regulation of lipid synthesis related genes, such as sterol regulatory element-binding protein (SREBP)-1, SREBP-2, SREBP-1c, fatty acid synthase (FASN), HMG CoA reductase (HMGCR), liver X receptor (LXR), acetyl-CoA carboxylase 1 (ACC1), hepatic CB (1) receptors, retinoid X receptor (RXR) α, were the main stay of liver steatosis pathogenesis. Degradation of lipid in liver is decreased in patients with CHC. There is strong evidence that heterogeneity of HCV core genes of different genotypes affect their effects of liver steatosis induction. A mechanism in which steatosis is involved in HCV life cycle is emerging.

Open access

Hui Li, Xiao-Dong Yang, Yong-Rui Yang, Si-Yuan Gao, De-Ying Tian and Quan Yuan

Abstract

Background and Aims Recently, epidemiology studies of hepatitis E in different areas are attracted more attention. Extensive studies of prevalent status and clinical manifestations could help us to broaden our knowledge, so as to excellently prevent and treat hepatitis E. The study analyzed the epidemiological characteristics and clinical features of 394 cases of sporadic acute hepatitis E (AHE) in Southwest of China from 2008 to 2010.

Methods The clinical data of 394 cases with sporadic AHE in Southwest of China from 2008 to 2010 were reviewed.

Results In the 394 cases with sporadic AHE, the ratio of male/female was 1.432 : 1, and the mean age was (31.53 ± 18.12) years. Totally, 94 (23.86%) patients aged under 18, 271 (68.78%) patients aged between 18 and 60, and 29 (7.36%) patients aged above 60. The incidence rate was significantly increased in summer (P = 0.000), especially in May (14.72%) and July (13.71%). In addition, the characteristics of occupation and ethnic group distribution were migrant laborers (106/394, 26.90%) and Han people (365/394, 92.64%). The length of stay, incidence of jaundice, the peak value of total bilirubin and alanine aminotransferase in male patients were all higher than those in female patients significantly (P < 0.05). The prolonged length of stay, decreased levels of ALT/ALB/CHE, increased levels of TBil, and increased incidence of jaundice and fatigue were associated with older age significantly (P < 0.05). The differences in peak values of total bilirubin (TBil), total bile acid (TBA), glutamyltransferase (GGT), cholinesterase (CHE) between AHE group and the groups of AHE accompanied respectively by chronic hepatitis B (CHB), acute alcoholic fatty liver (AFL), nonalcoholic fatty liver disease (NAFLD) were significant (P < 0.05). In addition, no significant difference was found in length of stay and biochemical indexes among anti-HEV-IgG positive group, anti-HEV-IgM positive group and anti- HEV-IgM/IgG both positive group (P > 0.05).

Conclusions Four epidemiological characteristics, including aged between 18 and 60, male, summer and migrant laborers, are found to be associated with acute hepatitis E. The prognosis of AHE in the majority of patients was favorable, but aged above 60 years and coexistence with CHB, AFL and NAFLD could be considerede as the factors inducing the infaust prognosis.

Open access

Cheng-bao Wang, Jian-jie Chen, Hong-ming Nie, Feng Gao, Hua Lv and Hong-ding Li

Abstract

Objective This study was undertaken to investigate the influencing factors on serum ALT level and hepatitis C virus (HCV) RNA titer in chronic hepatitis C (CHC) patients.

Methods All patients enrolled into this study were anti-HCV positive. Retrospective tracing method was applied to detect serum ALT level and HCV RNA titer and to collect general information of the patients such as genders, age groups, interferon medication history, infection pathways, height and weight. Then the multi-factor analysis was adopted with the application of binominal logistic regression mode.

Results The abnormal rate of ALT level was positively correlated to HCV RNA and gender while negatively correlated to interferon medication history and age group, with Wald value of the 4 factors as 39.604, 11.823, 18.991 and 7.389, respectively. The positive rate of HCV RNA was negatively correlated to interferon medication history and gender while positively correlated to ALT level, with corresponding Wald value of the 3 factors as 81.394, 7.618 and 27.562, respectively.

Conclusions The normal ALT level in HCV infected patients was associated with viral load, age, gender and interferon medication history, while the normal rate of HCV RNA titer was closely associated with gender, interferon medication history and ALT level.

Open access

Yun-ru Li, Feng-xin Chen, Xue-fei Duan, Xue-song Gao and Xiao-ling Fan

Abstract

A 50-year-old man with ankylosing spondylitis was treated successfully with infliximab, who was also a HBV carrier for about twenty five years. After injection with infliximab for four times, he developed jaundice and HBV DNA was detectable in serum. Serum aminotransferase and total bilirubin levels were higher than normal. Then he was hospitalized and treated with entacavir and Chinese herb medicine. But his liver damage aggravated and was diagnosed as acute on chronic liver failure. Finally, liver transplantation was carried out and he was cured successfully.

Open access

Ping Li, Xi Xiong, Xin Zhang, Zhi-guo Yang, Lei Gao and Mao-rong Wang

Abstract

Objective to investigate the association between HBV genotypes and characteristics of rtA181 mutation.

Methods Total of 85 chronic hepatitis B (CHB) patients who appeared rtA181 mutation after nucleos(t)ide analogs (NAs) therapy were enrolled in this study. Levels of serum ALT, AST, HBV DNA and HBsAg titers were monitored during therapy. HBV reverse transcriptase genes were amplified and sequenced to identify genotypes and resistance mutations. Virions and HBsAg in HepG2 cell with rtA181 mutation were also compared between genotypes B and C.

Results The majority of sera contained HBV genotypes B (15.7%) and C (84.3%). There were no significant difference of rtA181 mutant patterns between genotypes (P > 0.05). After emergence of rtA181 mutation, serum ALT, AST, HBV DNA levels and HBsAg titers were decreased than that at baseline (P < 0.05), while these characteristics were not different between genotypes B and C (P > 0.05). In cellular experiment, there were no significant differences between genotypes B and C not only in HBV virions but also in HBsAg titres (P > 0.05).

Conclusions No differences of clinical characteristics and cellular results were found in rtA181 mutation of HBV genotypes B and C.

Open access

Jiang Xiao, Yan-mei Li, Ying-xiu Huang, Wen Zhang, Wen-jing Su, Wei Zhang, Ning Han, Di Yang, Xin Li, Gui-ju Gao and Hong-xin Zhao

Abstract

Objective The aim of the study was to evaluate the characteristics of HIV drug-genotypic resistance among patients taking first-line ARV regimens using polymerase chain reaction and sequencing, and guide to design optimal ARV regimens for these patients.

Methods HIV reverse transcriptase-encoded gene was amplified with RT-PCR and amplified PCR products were aligned and comparatively analyzed with HIV resistance database to find drug-resistance mutations.

Results Twenty-eight PCR products were amplified and sequenced successfully in 30 serum samples of recruited HIV-infected patients with virologic failure. The resistance rate was 96%, mutations in NRT region were found in 26 patients (93%), while mutations in NNRT region were found in 27 patients (96%). M184V was the most common mutation (86%), K65R was selected in 14% of recruited individuals and TAMs occurred in 50% of patients, which resulted in resistance to NRTIs. Y181C and V179D were the most common mutations in NNRTIs and prevalence was 43% (12/28) and 36% (10/28), respectively, which resulted in cross-resistance to NNRTIs due to low-genetic barrier.

Conclusions Virologic failure may occur in long-term administration of first-line ARV regimens, and drugresistance mutations can be found in these patients, which resulted in resistance to first-line ARV regimens. We emphasized that HIV viral load assay and resistance assay were important tools to guide healthcare workers to design an optimal second-line ARV regimens for HAART-experienced individuals with virologic failure.