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  • Author: Krzysztof Zienkiewicz x
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Pollen Transcriptome and Proteome: Molecular and Functional Analysis


The pollen grain, because of its unique structural organization, is an extremely useful experimental model in cytological, molecular as well as in genetic studies. Due to the ease of pollen grain isolation, their sorting as well as simple extraction of their DNA, RNA and proteins, male gametophyte cells of angiosperms are presently one of the most intensively studied plant cells. Important and rapid progress in the development of experimental tools for genome exploration caused a significant increase in the number of reports concerning different aspects of gene expression during microsporogenesis and microgametogenesis in angiosperm plants. In this review we present the current knowledge of the pollen transcriptome and proteome during different stages of male gametophyte development, especially in Arabidopsis thaliana. Most of the results presented here were obtained in experiments carried out using microarrays, which were designed on the basis of the known sequence of the Arabidopsis genome.

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Application of Square Msplit Estimation in Determination of Vessel Position in Coastal Shipping


The main aim of this paper is to assess the possibility of using non-conventional geodetic estimation methods in maritime navigation. The research subject of this paper concerns robust determination of vessel’s position using a method of parameters estimation in the split functional model (Msplit estimation). The studies performed will help in finding out if and in which situations the application of Msplit estimation as the method for determining vessel’s position is beneficial from the perspective of navigation safety. The results obtained were compared with the results of traditional estimation methods, i.e. least squares method and robust M-estimation.

Open access