Search Results

1 - 2 of 2 items :

  • Author: Jan Marczuk x
Clear All Modify Search
Reproductive System Condition in Dairy Cows with Left-Sided Displacement of the Abomasums

Abstract

The aim of the study was to evaluate selected parameters representing reproductive system conditions in cows suffering from left-sided displacement of the abomasum (LDA). Eighty Holstein- Friesian cows were divided into control (n=40) and LDA (n=40) groups. Haematological and biochemical evaluations were performed in the control group and in cows with LDA before and after surgical treatment. Cytological and ultrasound examinations of the uterus were performed at 21 (baseline), 35 and 63 days after parturition. In the LDA group, significantly decreased concentrations of calcium, magnesium, phosphorus, copper, potassium, glucose and total cholesterol were stated, while aspartate aminotransferase activity, white blood cell count, erythrocyte count, haematocrit, haemoglobin content and concentration of β-hydroxybutyrate, free fatty acids and bilirubin were increased (all P<0.05). Percentage of neutrophils in the uterus and the uterus diameter were increased in the LDA group at the baseline and at 35 days after parturition (P<0.001). The neutrophil percentage was also increased at 63 days after parturition in the LDA group (P<0.001). Number of days to first oestrus, number of services per conception and calving-to-conception interval were higher in the LDA group (P<0.001). This study has shown reproductive system changes and impaired fertility in dairy cows as the consequence of LDA occurrence. Thus, LDA treatment in cows should be combined with diagnostic evaluation of the uterus and reproductive system to improve reproductive performance. As indicated in the cytological examination, a subclinical inflammatory process may last even 60 days after parturition, leading to substantial impairment of reproductive function in dairy cows.

Open access
Endometrial Cytology at Luteal and Follicular Phases of the Ovarian Cycle in Cows

Abstract

The aim of the study was to examine cytological changes in the uterus in cows during the follicular and luteal phases of the ovarian cycle, as well as to compare two different methods (brush and flushing) used for cytological material collection and to evaluate their usefulness for monitoring of the endometrium. Ovarian cycle phases were confirmed by ultrasound and by the level of sex hormones (17-β-estradiol and progesterone). The following types of cells were identified in the cytological smears: type I - surface cells; type II - intermediate cells; type III - basal cells; polymorphonuclear leukocytes (PMNs); L - lymphocytes. The number of type I and III cells was statistically significantly higher in the follicular phase than in the luteal phase, both in smears prepared using a brush (P<0.001) and by uterine flush (P=0.003). The number of type II cells was statistically significantly higher in the luteal phase than in the follicular phase in both methods (P<0.001). The results of the study show that phases of the ovarian cycle in cows can be identified based on changes in the quality and percentage of different types of endometrial cells in a cytological examination.

Open access