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  • Author: Ionela Dobrin x
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Observations on the Influence of Temperature Of Second Stage Juveniles (Meloidogyne Spp.) in the Absence of the Host Plant


The knowledge of root-knot nematodes behavior in different habitats and especially the knowledge of their reaction to major ecologic factors (humidity, temperature) are important for the assessment of pathogen potential in a specific area, being at the same time an important criterion for understanding the behavior towards host plants. The temperature is the most important factor, not just in the development of the entire biological cycle of root-knot nematodes, but at the same time it also affects their distribution, spread and survival under thermic stress conditions (excessive heat or cold). Alongside with the water and oxygen, the temperature is the triggering factor of hatching and running all stages until the adult stage. The articles aims to determine the survival of juveniles stages for Meloidogyne species, in the absence of host plants, and by exposing second stage juveniles to different temperatures. The juveniles survival and mobility decreases quite much at relative low temperatures (4°C and 10°C). Forward movements, mobility, hatching and lifetime were accentuated at higher temperatures (20°C) for a few weeks, so that after a period to decrease drastically (in 10 and 12 week) for the species considered for the study.

Open access
The Relevance of Oxidative Stress Status in One Week and One Month Alcohol Abstinent Patients


Background: Although it is generally accepted that there is an increased oxidative stress status in alcoholics, the separate relevance of oxidative stress following alcohol withdrawal is still not understood to this date. There are reports stating that the increased oxidative stress status in alcoholics may persist independently of the constant presence of alcohol intake, while on the other side, it was demonstrated that the antioxidant defense mechanism could significantly increase after alcohol withdrawal.

Methods: In the present work, we were interested in studying the relevance of oxidative stress status in the alcohol withdrawal processes, by determining some oxidative stress markers (two antioxidant enzymes: superoxide dismutase - SOD and glutathione peroxidase - GPX and a lipid peroxidation maker - MDA) after one week and one month of abstinence, as compared to the baseline and a control group of subjects.

Results: Our data confirmed the increased oxidative stress status in alcoholic patients and, more importantly, we de m - onstrated here a significant decrease of the oxidative stress status one week and one month following the withdrawal, as showed by a significant increase in the specific activity of SOD (p<0.003), as well as by a decrease in MDA levels (p<0.019). Still, in the case of all three markers of oxidative stress status which we determined, the levels after one week or one month of abstinence were significantly altered when compared to controls.

Conclusions: This suggests that severe and prolonged deficiency in the oxidative stress marker levels needs longer than one month of abstinence to normalize.

Open access