Study aim: The study is quantitative, with a cross-sectional comparison design, and aimed to verify the association between physical activity (PA) levels and biochemical markers linked to liver function of patients infected with hepatitis C virus (HCV). Material and methods: The following biochemical variables were analyzed: alanine aminotransferases (ALT), gamma-glutamyl transpeptidase (γGt), and alkaline phosphatase (FA), these being good markers for hepatic damage, besides glycemia, HDL, LDL, and triglycerides. The International Physical Activity Questionnaire (IPAQ), short version, was used to evaluate the levels of physical activity, being classified as active and physically inactive. Results: The sample consisted of 84 individuals aged 56.2 ± 11.6 years; there was a predominance of males, patients with different socioeducational patterns and those followed up for more than 6 months in the hepatology service with positive serology for HCV and HCV (RNA+), attending the Institute of Liver and Transplants of Pernambuco (IFP). The results obtained in this study indicate smaller values of the median with maximum and minimum scores of the analyzed parameters in patients considered to be physically active (ALT: p = 0.43, FA: p = 0.86 and γGt: p = 0.15, glycemia: p = 0.22, LDL: p = 0.58, HDL: P = 0.004, triglycerides: p = 0.47), in comparison with the physically inactive patients. It is concluded that patients who have a higher level of physical activity present lower values of biochemical markers, positively affecting their hepatic condition. Conclusions: There was only a significant difference in the HDL variable, but the patients with a lower level of physical activity showed greater values in general, less in the triglyceride variability. In order to test these and other important variables and improve the quality of life and health by a more direct method.