The paper addresses complex evaluation of safety culture in Slovak manufacturing enterprises by utilizing specific determinants. To accommodate conditions of enterprises in Slovakia, a specific research method was developed for the study of safety culture, which can also be used for manufacturing and non-manufacturing enterprises. We have selected the questionnaire method. Due to the scope of the research, we have defined ten determinants, which form the basis for evaluation of safety culture elements. When determining the level of the specific ten determinants of safety culture, we studied interdependence between the enterprise’s industry, its size (small, medium, large) and points assigned to each determinant. The results of the study are evaluated using a 3D point graph. The discussion shows parts that were evaluated the highest or lowest and outlines suggested approaches to improve the current state.