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  • Author: Davoud Kianifard x
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Davoud Kianifard


Background and aims: Diabetic hyperglycemia leads to structural and functional alterations in body organs including testis. Monosodium glutamate (MSG) is a food additive which has toxic effects on human and animal’s tissues. The aim of this study was to evaluate the effects of MSG on diabetic complications of testicular tissue.

Material and Methods: 48 adult rats were divided into six groups. Diabetes was induced by Streptozotocin (45 mg/kg i.p.). Monosodium glutamate was administrated in two doses (6 and 60 mg/kg) to control and diabetic groups. After eight weeks, the body weight was measured and blood samples were collected for analysis of the pituitary-testicular axis hormones. Formalin fixed tissue samples were prepared through routine histologic methods.

Results: MSG led dose dependently to weight gain of control groups and prevented weight loss of diabetic rats. The blood levels of luteinizing hormone and testosterone were reduced in diabetic rats following administration of MSG. Reduction of cellular population of germinal epithelium, seminiferous tubules diameter decrement and decrease of indices of spermatogenesis were observed with more intensity in MSG treated diabetic rats.

Conclusions: The results of this study suggest that the long term administration of MSG may induce in a dose dependent manner structural and functional alterations of testicular tissue in diabetic rats.

Open access

Davoud Kianifard and Farhad Rezaee


Background and Aims: Various types of infertility are associated with uncontrolled hyperglycemia and diabetes. Development of oxidative stress is one the most important factors in the alteration of spermatogenesis in diabetic conditions. Consequently, the reduction of oxidative stress with antioxidant compounds can be effective in the reduction of tissue alterations. The aim of this study was to evaluate the efficacy of coenzyme Q10 in improvement of spermatogenesis in adult diabetic rats. Material and Methods: 32 adult rats were divided into four groups of control and treatment. Coenzyme Q10 (10 mg/kg body weight - b.w.) was administrated to one control and one diabetic (intraperitoneal injection of 45 mg/kg b.w. of Streptozotocin) groups. Blood concentrations of FSH, LH and Testosterone were measured. Histology of testicular tissue and sperm analysis were considered for evaluation of spermatogenesis. Results: Administration of Coenzyme Q10 led to increase of pituitary gonadotropins levels in diabetic rats. Testosterone levels were not changed significantly. Testicular morphology, spermatogenic indices and sperm analysis were improved in treated diabetic rats. Conclusions: The results of this study suggest that the use of Coenzyme Q10 has positive effects in reduction of spermatogenic alterations following induction of experimental diabetes in rats.

Open access

Davoud Khar Razi, Davoud Kianifard and Emad Khalilzadeh


Background and aims: Endogenous opioids function as negative factors affecting the growth has been established. The most influential factor in the growth and differentiation of the proliferating cells is the opioid growth factor (OGF). Recently, some studies have been completed about the effects of opioid growth factor in the pathogenesis of diabetes and the beneficial effects of inhibition of this growth pathway have been demonstrated. The aim of this study was to investigate the effect of inhibition of opioids growth pathway, in proliferation and growth of testicular germ cells and spermatogenesis following experimental diabetes in adult mice.

Material and methods: Diabetes was induced in adult mice by Streptozotocin. Diabetic animals were treated with Naltrexone 15, 30 and 60 mg/kg for 60 days. At the end of the study, testicular and body weight was recorded, tissue samples were collected and histomorphometrical studies were performed under light microscope.

Results: The results showed that the use of naltrexone has a little effect on preventing diabetic weight loss. Histomorphometric indices such as tubular diameter and germinal epithelium height were improved dose dependently in naltrexone treated diabetic mice. The number of tubular germ cells was increased non-significantly in diabetic animals following administration of naltrexone. Improvement of microscopic indices of spermatogenesis was observed in naltrexone treated diabetic mice.

Conclusions: According to the results of this study and the role of naltrexone as OGF-OGF receptor inhibitor and up-regulating activity of naltrexone which leads to increased DNA synthesis and cell division process, the administration of naltrexone could be effective in reduction of diabetic induced alterations of spermatogenesis.

Open access

Davoud Kianifard, Gholamreza Vafaei Saiah and Farhad Rezaee


Background and Aims: Starting from the cytotoxic effects of monosodium glutamate (MSG), the aim of this study was to evaluate the protective effects of quince leaf extract as natural antioxidant on the reproductive dysfunction induced by monosodium glutamate in rats. Material and methods: Monosodium glutamate was administrated with a dose of 30 and 60 mg/kg and quince leaf extract was administrated with a dose of 500 mg/kg. At the end of study, body and testicular weight measurement, hormonal and epididymal sperm analysis were performed. Results: Follicle stimulating hormone (FSH) and testosterone levels were reduced after administration of monosodium glutamate. The levels of luteinizing hormone (LH) exhibited no significant changes. Treatment with quince leaf extract led to improvement in follicle stimulating hormone and testosterone levels. Epididymal sperm population was reduced after administration of monosodium glutamate and treatment with quince leaf extract. The increased sperm motility rate induced by monosodium glutamate was reduced after treatment with quince leaf extract. Administration of monosodium glutamate led to more body weight gain in comparison to combined administration monosodium glutamate and quince leaf extract. Conclusions: The quince leaf extract can be effective in reduction of functional alterations of reproductive system induced by monosodium glutamate.