Ultraviolet radiation (UV) altered plant metabolism. Hence Trigonella foenum-graecum L. (Fenugreek) exposed to UV-B radiation for studying the bioactive changes that may be useful in captive farming. UV-B treatment altered plant growth, and extent of alterations depended on the duration of radiation treatment. Photosynthetic pigments such as chlorophyll and carotenoids decreased after radiation exposure. But bioactive components such as anthocyanin, flavonoids, and phenolics increased after UV-B treatment. Phenylalanine lyase enzyme activity and peroxidase activity also increased with 4.0 hr UV-B exposure even though 8.0 hr exposure decreased the activity of these enzymes. Total lipid content of the plants increased after UV-B exposure. Changes in aromatic oil composition observed due to UV-B exposure, and the changes pointed shifting of plant metabolism towards the synthesis of short chain fatty acid contain lipids and non-enzymatic antioxidants.