Botanical gardens have diverse habitats and floristic conditions. The aim of this study was to examine whether these specific environmental conditions have a positive impact on the appearance of mushrooms. Between 2011 and 2013, mycological observations were performed in the Botanical Garden of the University of West Hungary, Sopron. A total of 171 mushrooms species were identified. Several rare species and two protected species were found. The identification and classification of the species reveal how botanical gardens provide a special habitat for mushrooms. These features of botanical gardens are beneficial for fungal dissemination and preservation.