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  • Autor: Slavomír Čerňanský x
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Influence Of Fine-Grained Montmorillonite On Microfungal Pellets Growth In Aqueous Suspensions

Abstract

The paper presents an inhibition effect of clay mineral – montmorillonite – on the growth of microscopic filamentous fungus Aspergillus niger in the aqueous solution. The significant reduction in growth of the final size of spherical fungal pellets as well as total amount of produced microbial biomass was found out. Within the observed range of additions of clay mineral of 1, 5, 10, 15 and 20 g in the total volume of the 80 ml suspension, this size was in indirect relation to the weight of montmorillonite. However, the most significant inhibition effect was observed at the lowest concentration of the sorbent (1 g). Microscopic analysis of pellets referred to the presence of mineral particles in their pore structure and the distribution of particles in the spatial structure of fungal hyphae was variable. The experiment clearly demonstrated an inhibition effect of montmorillonite. This inhibition could be answered by the experiments focused on the detection of the influence of size and shape of inorganic sorption particles together with the influence of the physicochemical properties of its surface. It could be stated that the simultaneous application of the microscopic fungus Aspergillus niger and the clay mineral montmorillonite for decontamination of waste waters should be disadvantage due to their interaction if compared with the decontamination based on bioaccumulation and sorption separately.

Open access
Bioleaching of Arsenic and Antimony from Mining Waste

Abstract

This paper is a contribution to quantification of bio-leached arsenic and antimony from mining waste collected from impoundment in Slovinky (Slovakia). Autochtonous fungal strain Aspergillus niger was used in all bioleaching experiments. The contents of arsenic and antimony in two different samples from the impoundment were 294.7 and 328.2 mg.kg−1 As and 225.3 and 285.7 mg.kg−1 Sb, respectively. After 21-day cultivation of Aspergillus niger on such contaminated substrates, this strain was capable to bioleach, bioaccumulate and biovolatilize both toxic elements.

Open access
Responses of Aspergillus niger to selected environmental factors

Abstract

Four wild type strains of A. niger were collected from soil and stream sediments representing environments with variable level of As, Sb, Al, Fe, Cd, Cu, and Zn contamination. Banská Štiavnica-Šobov (S), Pezinok-Kolársky vrch (P) and Slovinky (Sl) represent contaminated localities. Locality Gabčíkovo (G) was as a control site. The influence of toxic elements in these substrates on fungal growth, colony size, enzymatic activity, production of organic acids and their pelletization in water suspensions with montmorillonite was studied. The aim of our study was to find out how the wild type strains from (contaminated) environment will behave in different model solutions. We also wanted to add some new information in this area of study, because that there is some gap in the available knowledge.

Open access