In total, 14 608 pig sera, collected between 2008 and 2011, were tested with ELISA using antibodies specific for bovine viral diarrhoea virus (BVDV). All doubtful and positive samples were retested by virus neutralisation test (neutralising peroxidase-linked assay). The BVDV seroreagents were detected in 11 (68.75%) out of 16 provinces, the seroprevalence varied from 0.1% to 1.04% (average 0.31%). The obtained results indicate that the prevalence of BVDV infection in pig population in Poland is low.
The aim of the study was to analyse the prevalence of single-reactor (SR) pigs in Poland, to attempt an explanation for this phenomenon, and to assess whether the occurrence of SR pigs could create problems for a successful Aujeszky’s disease virus (ADV) eradication programme in Poland. A total of 6494 ADV gE antibody positive/doubtful sera were retested by gB ELISA and subsequently by virus neutralisation test (VNT) to confirm the results of the glycoprotein E (gE) ELISA. Amongst the serum samples tested, 5.9% could be classified as being taken from SR pigs, as was shown by gE ELISA positive/doubtful results, which were not confirmed by negative findings in gB ELISA and VNT. It means that the observed SR phenomenon was due to a false positive/doubtful reaction in gE ELISA. This finding was strongly supported by the fact that the serum samples were taken from the animals from herds without any previous or subsequent history of Aujeszky’s disease. The low percentage of SR pigs does not seem to create a big obstacle to a successful ADV eradication programme in Poland.
Andrzej Lipowski, Anna Szczotka-Bochniarz and Zygmunt Pejsak
Introduction: Aujeszky’s disease virus (ADV) infects a wide range of animals, including members of the Suidae family, i.e. domestic and wild pigs, as well as wild boar. Since wild boar are a potential ADV reservoir and a source of infection for domestic pigs, the aim of the study was to evaluate ADV antibody prevalence in the Polish wild boar population, during the years 2011 to 2014.
Material and Methods: Wild boar blood samples were collected during three consecutive hunting seasons; i.e. 2011/2012, 2012/2013, and 2013/2014, and tested for ADV antibodies by ELISA.
Results: ADV antibodies were detected in samples from all tested voivodships. The average seroprevalence reached 32.2%. Seroprevalence, over the examined hunting seasons, was 27.4% in 2011/2012, 32.4% in 2012/2013, and 35.5% in 2013/2014. The highest percentage of seroreagents was detected in four voivodships, situated along the western border of Poland, i.e. Zachodnio-Pomorskie (ZP), Lubuskie (LB), Dolnośląskie (DS), and Opolskie (OP). This area is positively correlated with the highest density of the wild boar population and the highest wild boar hunting bag.
Conclusion: The results of this study confirm that the wild boar population may still pose a threat to domestic pigs, which is of special importance at the final stage of Aujeszky’s disease eradication programme in Poland.
Anna Szczotka-Bochniarz, Andrzej Lipowski, Anna Kycko, Bartosz Sell, Michał Ziółkowski and Barbara Małek
Introduction: Aujeszky’s disease (AD), most often related to infection of domestic and feral swine, may also concern other mammals, including dogs. The disease in carnivores, related to consumption of raw meat or offal contaminated with AD virus, is manifested by severe neurological disorders and inevitably leads to animal’s death.
Material and Methods: Karelian bear dog was euthanised due to nervous symptoms that started two days after participation in wild boar hunting. After exclusion of rabies the dog’s carcass was subjected to standard necropsy. Tissue samples were collected for histological examination. Samples of the brain were tested for ADV by real-time PCR and virus isolation. Samples of the liver were collected for toxicological examination.
Results: The presence of ADV was confirmed by real-time PCR and virus isolation. Toxicological examination revealed anticoagulant poisoning. This is the first case of Aujeszky’s disease (AD) in a hunting dog in Poland after exposure to ADV from offal of wild boar.
Conclusion: This infection should be taken into consideration in differential diagnosis of syndromes of neurological disorders in dogs. Since AD is found in both domestic pigs and wild boar in Poland, special care must be taken to prevent spread of infection to other species.