Research indicates that exposure to microgravity leads to immune system dysregulation. However, there is a lack of clear evidence on the specific reasons and precise mechanisms accounting for these immune system changes. Past studies investigating space travel-induced alterations in immunological parameters report many conflicting results, explained by the role of certain confounders, such as cosmic radiation, individual body environment, or differences in experimental design. To minimize the variability in results and to eliminate some technical challenges, we advocate conducting thorough feasibility studies prior to actual suborbital or orbital space experiments. We show how exposure to suborbital flight stressors and the use of a two-dimensional slow rotating device affect T-cells and cancer cells survivability. To enhance T-cell activation and viability, we primed them alone or in combination with IL-2 and IL-12 cytokines. Viability of T-cells was assessed before, during the experiment, and at the end of the experiment for which T-cells were counted every day for the last 4 days to allow the cells to form clear structures and do not disturb their evolution into various geometries. The slow rotating device could be considered a good system to perform T-cell activation studies and develop cell aggregates for various types of cells that react differently to thermal stressors.
Arayik Martirosyan, Lawrence J. DeLucas, Christina Schmidt, Markus Perbandt, Deborah McCombs, Martin Cox, Christopher Radka and Christian Betzel
To investigate the effect of macromolecular transport and the incorporation of protein aggregate impurities in growing crystals, experiments were performed on the International Space Station (ISS) and compared with control experiments performed in a 1G laboratory environment. Crystal growth experiments for hen egg-white lysozyme (HEWL) and Plasmodium falciparum glutathione S-transferase (PfGST) were monitored using the ISS Light Microscopy Module (LMM). Experiments were performed applying the liquid–liquid counter diffusion crystallization method using rectangular, optically transparent capillaries. To analyze the quantity of impurity incorporated into growing crystals, stable fluorescently labeled protein aggregates were prepared and subsequently added at different percent concentrations to nonlabeled monomeric protein suspensions. For HEWL, a covalent cross-linked HEWL dimer was fluorescently labeled, and for PfGST, a stable tetramer was prepared. Crystallization solutions containing different protein aggregate ratios were prepared. The frozen samples were launched on 19.02.2017 via SpaceX-10 mission and immediately transferred to a -80°C freezer on the ISS. Two series of crystallization experiments were performed on ISS, one during 26.02.2017 to 10.03.2017 and a second during 16.06.2017 to 23.06.2017. A comparison of crystal growth rate and size showed different calculated average growth rates as well as different dimensions for crystals growing in different positions along the capillary. The effect of macromolecular mass transport on crystal growth in microgravity was experimentally calculated. In parallel, the percentage of incorporated fluorescent aggregate into the crystals was monitored utilizing the fluorescent LMM and ground-based fluorescent microscopes.
The sieves’ manufacturers that want to be competitive at the market must have the implemented quality control system. Different methods, based on taking the sample, which is a representative of the total quantity of the forming sieves production, are applied for evaluation of the sieve’s conformity. The basic characteristics of this type of the quality function are control, measuring and testing of the forming sieves’ properties at the end of the manufacturing process. The objective is to detect and separate the products that deviate from the set criteria, to remain in the factory, then to be repaired if possible or discarded in the opposite case and thus to prevent delivery of the defective sieves to the paper manufacturers.
Nanocrystalline zinc sulfide thin films were prepared on glass substrates by chemical bath deposition method using aqueous solutions of zinc chloride, thiourea ammonium hydroxide along with non-toxic complexing agent trisodium citrate in alkaline medium at 80 °C. The effect of deposition time and annealing on the properties of ZnS thin films was investigated by X-ray diffraction, scanning electron microscopy, optical transmittance spectroscopy and four-point probe method. The X-ray diffraction analysis showed that the samples exhibited cubic sphalerite structure with preferential orientation along 〈2 0 0〉 direction. Scanning electron microscopy micrographs revealed uniform surface coverage, UV-Vis (300 nm to 800 nm) spectrophotometric measurements showed transparency of the films (transmittance ranging from 69 % to 81 %), with a direct allowed energy band gap in the range of 3.87 eV to 4.03 eV. After thermal annealing at 500 °C for 120 min, the transmittance increased up to 87 %. Moreover, the electrical conductivity of the deposited films increased with increasing of the deposition time from 0.35 × 10−4 Ω·cm−1 to 2.7 × 10−4 Ω·cm−1.
The present research is focused on developing ZnAl2O4 (gahnite) spinel as an antireflection coating material for enhanced energy conversion of polycrystalline silicon solar cells (PSSC). ZnAl2O4 has been synthesized using dual precursors, namely aluminum nitrate nonahydrate and zinc nitrate hexahydrate in ethanol media. Diethanolamine has been used as a sol stabilizer in sol-gel process for ZnAl2O4 nanosheet fabrication. ZnAl2O4 nanosheet was deposited layer-by-layer (LBL) on PSSC by spin coating method. The effect of ZnAl2O4 coating on the physical, electrical, optical properties and temperature distribution in PSSC was investigated. The synthesized antireflection coating (ARC) material bears gahnite (ZnAl2O4) spinel crystal structure composed of two dimensional (2D) nanosheets. An increase in layer thickness proves the LBL deposition of ARC on the PSSC substrate. The ZnAl2O4 2D nanosheet comprising ARC on the PSSC was tested and it exhibited a maximum of 93 % transmittance, short-circuit photocurrent of 42.364 mA/cm2 and maximum power conversion efficiency (PCE) 23.42 % at a low cell temperature (50.2 °C) for three-layer ARC, while the reference cell exhibited 33.518 mA/cm2, 15.74 % and 59.1 °C, respectively. Based on the results, ZnAl2O4 2D nanosheets have been proven as an appropriate ARC material for increasing the PCE of PSSC.
Sachin Kumar, Naven Kumar, Kamna Yadav, Annveer and R.P. Singh
DFT analyses of electronic and optical spectra of barium cadmium chalcogenides (Ba2CdX3, X = S, Se, Te) have been carried out. The study of electronic spectra has been made in terms of band structure and density of states using full potential linear augmented plane wave plus local orbital method. Band structure calculations have been carried out under the approximations PBE-GGA, PBE-Sol, LDA and TB-mBJ. Band structures of these materials show that Ba2CdS3, Ba2CdSe3 and Ba2CdTe3 crystals possess a band gap less than 1 eV, underestimated relative to the experimental/theoretical literature values. Optical spectra of these chalcogenides have been analyzed in terms of real and imaginary parts of dielectric function, reflectivity, refractive index, extinction coefficient, absorption coefficient, optical conductivity and electron energy loss. Optical results show large anisotropy along different directions. These results provide a physical basis of barium cadmium chalcogenides for potential application in optoelectronic devices.
This is a study of a medical injection factory-Babylon carried out in order to achieve proper mechanical and morphological properties, PP has been injection molded by using cold runner injection molding machine with temperature variation (198, 200, 203……220°C) for ten samples. The physical and mechanical properties of PP product were examined. It has been found that the Shore hardness decreases linearly with injection molding temperature increasing. The tensile strength has a similar behavior to the hardness. However, it has been found that the MIF (Melt Index Flow) rates increases with the increase of injection molding temperature. The density of PP has been found for both virgin PP and the samples, it has been found that the density decreases with increasing operation temperature. FTIR (Fourier Transmission Infrared) spectra were taken for both samples with high and low operation temperature. Besides the SEM (Scanning Electronic Microscopy) test shows the difference in the morphology of the product surface and the PP product at high and low operation temperature. Moreover, for all these properties, the PP product exhibits good mechanical properties (hardness, tensile strength, density) for the samples produced at temperature lower than 207°C. While the physical properties such as MIF improved with injection temperature increasing, additionally, the SEM images show that the sample produced in low temperature have surface damage.
Setia Budi, Sukro Muhab, Agung Purwanto, Budhy Kurniawan and Azwar Manaf
The effect of electrodeposition potential on the magnetic properties of the FeCoNi films has been reported in this paper. The FeCoNi electrodeposition was carried out from sulfate solution using potentiostatic technique. The obtained FeCoNi films were characterized by X-ray diffractometer (XRD), atomic absorption spectrometer (AAS) and vibrating sample magnetometer (VSM). It has been shown that the electrodeposition potential applied during the synthesis process determines the magnetic characteristics of FeCoNi films. The more negative potential is applied, the higher Ni content is in the FeCoNi alloy. At the same time, Co and Fe showed almost similar trend in which the content decreased with an increase in applied potential. The mean crystallite size of FeCoNi films was ranging from 11 nm to 15 nm. VSM evaluation indicated that the FeCoNi film is a ferromagnetic alloy with magnetic anisotropy. The high saturation magnetization of FeCoNi film was ranging from 86 A·m2/kg to 105 A·m2/kg. The film is a soft magnetic material which was revealed by a very low coercivity value in the range of 1.3 kA/m to 3.7 kA/m. Both the saturation magnetization and coercivity values decreased at a more negative electrodeposition potential.
Katarína Stachová, Zdenko Stacho, Zuzana Papulová and Marek Jemala
The need for adaptation to rapid changes in the business environment, both on the part of employees and employers, implies dealing with new challenges, acquisition of new knowledge and skills and assumption of new roles and responsibilities. The base for companies is the availability of qualified human resources, which is ensured by the human resource management department mainly through an effective process of employee selection. The paper is oriented at the analysis of the current state of focus of organizations operating in Slovakia on systematicity and complexity of the selection process, the existence of an effective internal labor market and the regularity of development of used selection tools in the context of technological progress and changes in labor market requirements in regions of Slovakia. Results of the survey on (n = 343) enterprises show a positive trend, an increase of more than 10 percent in the orientation towards improvement of the process of employee selection, but on the other side it also confirm an increase in disparities between individual regions of Slovakia, some regions (Eastern Slovakia) progress much slower than the developed ones (Bratislava).
Fucheng Yu, Hailong Hu, Bolong Wang, Haishan Li, Tianyun Song, Boyu Xu, Ling He, Shu Wang and Hongyan Duan
Al doped ZnO (AZO) thin films were prepared on silica substrates by sol-gel method. The films showed a hexagonal wurtzite structure with a preferred orientation along c-axis. Suitable Al doping dramatically improved the crystal quality compared to the undoped ZnO films. Dependent on the Al dopant concentration, the diffraction peak of (0 0 2) plane in XRD spectra showed at first right-shifting and then left-shifting, which was attributed to the change in defect concentration induced by the Al dopant. Photocatalytic properties of the AZO film were characterized by degradation of methyl orange (MO) under simulated solar light. The transmittance of the films was enhanced by the Al doping, and the maximum transmittance of 80 % in the visible region was observed in the sample with Al concentration of 1.5 at.% (mole fraction). The film with 1.5 at.% Al doping achieved also maximum photocatalytic activity of 68.6 % under solar light. The changes in the film parameters can be attributed to the variation in defect concentration induced by different Al doping content.