The arylidene indanone scaffold has contributed many lead molecules in chemotherapeutic anticancer agent research.
To determine the oxidant-scavenging activities and antiproliferative activity of (2E)-2-benzylidene-4,7-dimethyl-2,3-dihydro-1H-inden-1-one (MLT-401), an arylidene indanone derivative.
Jurkat cells, primary lymphocytes, and Vero cells were treated with MLT-401. Antioxidant properties of MLT-401 were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH)-based, 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS)-based, and ferric-reducing antioxidant potential (FRAP) assays. Inhibition of cell proliferation was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide-based assay. Nuclear status was determined using a DNA fragmentation assay, and cell cycle stage was analyzed by flow cytometry. Mitochondrial membrane enzyme activities were measured using colorimetric methods.
The antioxidant assays gave MLT-401 half maximal inhibitory concentration (IC50) values of 1611 nM (DPPH-based assay), 2115 nM (ABTS-based assay), and 1586 nM (FRAP assay). MLT-401 inhibited proliferation of Jurkat cells with a concentration for 50% of maximal inhibition of cell proliferation (GI50) of 341.5 nM, being 12- and 9-fold less than GI50 concentrations for normal lymphocytes and Vero cells, respectively. MLT-401 caused nuclear fragmentation and DNA laddering as seen by electrophoresis. Jurkat cells showed a time-dependent accumulation of sub G0/G1 cells after MLT-401 treatment. Mitochondrial membrane-bound Na+/K+ ATPase, Ca2+ ATPase, and Mg2+ ATPase activities were inhibited by MLT-401 in a dose-dependent manner.
MLT-401 possesses significant antiproliferative activity and scavenges free radicals released through mitochondrial membrane damage in a Jurkat cell line model of cancer cells. Further investigation of MLT-401 as a chemotherapeutic anticancer agent and development of other arylidene indanone analogues are warranted. A detailed elucidation of mechanistic pathways is required for further development.
Serum starvation is mostly considered as a standard preparatory method in many cellular and molecular experiments. However, recent studies give some evidence that serum starvation is a major event that triggers various cell responses and has therefore great potential to change and interfere with the experimental results. In this study, the behavior of breast cancer cells in serum-starved condition was examined.
To focus on the role of serum starvation on cell migration and also the possible changes in the expression and secretion of genes and cytokines mostly involved in migration and chemotaxis of breast cancer cells.
MDA-MB-231 cells were cultured under serum-starved condition. Transwell migration assay was performed to evaluate the effect of serum starvation on cell migration after 24, 48, and 72 h. The transcriptional expression of migration-related genes was evaluated using real-time polymerase chain reaction. The cytokine secretion was also analyzed using enzyme-linked immunosorbent assay.
Serum starvation suppressed cell migration in breast cancer cells. Additionally, the gene expression of markers involved in migration including β-catenin, twist, zinc finger E-box binding homeobox 1, vimentin, fibronectin, intercellular adhesion molecule 1, and vascular endothelial growth factor were downregulated. Moreover, cytokines of transforming growth factor, beta 1, matrix metallopeptidase 9, interleukin 8, and nitric oxide were differentially secreted.
Serum deprivation causes significant changes in cancer cell migration and also the expression of migration-related genes and cytokines, special care needs to be taken when this practice is used as preparatory method especially in migration and chemotaxis experiments on cancer cells.
Multidrug-resistant bacteria are becoming more hazardous day by day for human health all over the world, and the scientific community is trying hard to resolve this issue by various approaches. One of the very common approaches is to bind drugs to nanoparticles and study enhanced antibacterial properties.
To compare simultaneously different types of nanoparticles, their concentration, bacterial strains and their incubation time intervals for each of the selected drug combination.
We have selected the most commonly used gold and silver nanoparticles and few examples from fluoroquinolone antibiotics to make their conjugates and study their efficacy against multidrug-resistant E. coli and S. aureus strains simultaneously, at different incubation time intervals and different concentration of nanoparticles.
Gold nanoparticle hybrids do not show any significant effect. Silver nanoparticle hybrids show far better results, even at extremely low concentrations.
This unique and simple approach allows us to know the exact time intervals and concentration required for each nanoparticle combination to control the growth for any specific strain. This approach can be extended to any set of nanoparticles, drugs and bacterial strains for comparative purposes.
Simulation is widely used in airway management training.
To show that assigning anesthesia residents’ simulation educator roles improved cognitive learning outcomes.
Postgraduate second- and third-year (PGY-2 and PGY-3) anesthesia residents were randomly assigned to three groups: a teacher group (T), a hot-seat (active participant) group (H), and an observer group (O). After a train-the-trainer session, the T group prepared simulation scenarios for difficult airway management and then conducted the simulation sessions and post-session debriefing. The H group participated in the scenarios, and the O group observed the sessions. All participants attended the post-session debriefing. Evaluation was conducted at pretest, immediate posttest, and 3 months (retention test). Score differentiation and average normalized gain were calculated. Participants completed a post-simulation class survey.
Participants were 49 residents (PGY-2 = 24, PGY-3 = 25). The T group had the highest posttest score (17.06 ± 1.23); this score significantly differed from the O group (14.75 ± 2.57, P = 0.003) but not the H group (15.64 ± 1.54, P = 0.103). The average normalized gain was significantly higher in the T group than in the H and O groups (0.51 ± 0.22, 0.18 ± 0.32, and 0.17 ± 0.47, respectively; P = 0.012). Participants retained knowledge at 3 months after the session, with no significant differences among the groups. Most participants (45%) preferred to be active scenario participants, and 20% preferred to teach. Overall satisfaction was high in all groups.
This study showed that a teaching role can be effectively applied for residents in simulation-based education on difficult airway management to support better learning outcomes.
Acute liver failure (ALF) is a rare condition during neonatal period.
To report a case of recipient twin with fulminant ALF secondary to hydrops fetalis caused by twin-to-twin transfusion syndrome (TTTS).
The patient was admitted to the neonatal intensive care unit (NICU) for respiratory failure requiring mechanical ventilation and fulminant ALF with prolonged international normalized ratio (INR) and elevated liver enzymes with highest aspartate aminotransferase of 4,580 U/L.
Laboratory investigation for secondary causes of liver failure was not revealing. Her liver enzymes and coagulation levels were dramatically normalized as the clinical symptoms of hypervolemia improved within 1 week.
TTTS can be a possible cause of neonatal ALF. Early detection with proper management of TTTS is important to avoid adverse outcomes. However, pathogenesis of hepatic dysfunction in TTTS is rarely described, and further studies are needed to help understanding the correlation between liver diseases and TTTS.
A field experiment was carried out in sub-tropical climate of Bhimdattanagar, Kanchanpur to assess the effect of site specific nutrient management approach in productivity of spring rice. The experiment was carried in single factor randomized completely block design with four replication, in four different farmer’s field, each farmers’ field as single replication. The seedlings were grown in the mat nursery bed and transplanted in the experimental plots with different nutrient management practices. Nutrient management practices were Site Specific Nutrient Management with Nutrient Expert dose (SSNMNE) (109:28:38 kg NPK/ha), Government recommendation dose (100:30:30kg NPK/ha +6 ton FYM), Farmer’s field practice (FFP)(different among farmers) with three omission plots viz. omission of Nitrogen (0:28:38 kg NPK/ha), Omission of Phosphorus (109:0:38 kg NPK/ha) and Omission of Potassium (109:28:0 kg NPK/ha). SSNM-NE dose was obtained by household survey in the respective area and using nutrient expert model. The growth parameters, yield, yield attributing traits and total cost of production were recorded. Data were analyzed using Duncan’s Multiple Range Test (DMRT) in Genstat. Result indicates that the highest grain yield (5.81 t/ha) was obtained from SSNM-NE which was statistically at par with government recommendation (5.54 t/ha). Similarly, thousand grains weight, straw yield in the experiment 23.20g, 6.90t/ha were significantly higher in SSNM-NE respectively. Straw yield, grain yield and thousand grain weight of SSNM-NE were found to be statistically at par with government recommendation. The economic analysis revealed that higher B: C ratio (1.91), net return and gross return were higher in SSNM-NE.
This review tends to analyze the importance of halal animal feed from the Islamic standpoint and describes the drawbacks of non-halal. Mischievousness to animal handling during production, transport, and slaughter in states where Islam is the main doctrine has created doubts about the quality of products. Islam is a religious conviction that illustrates empathy to animals and well-being of humankind food as mentioned in the holy book Qur’an and sayings of the Prophet Mohammed (PBUH). In a variety of animal-based halal food products, a Muslim should be conscious of not only about the rearing organization of the animals but also the slaughtering procedure. Animal feed is the preliminary Critical Control Point to establish the halal authenticity of animal-based food products. Regardless of the recognized agenda on animal feed in Middle East Asia, impurity in animal feed ingredients is still occurring and repetitively reported by the media. Feed inflicts direct effect on the animal originated feedstuffs like meat, milk, and eggs and it has been acknowledged as the foremost cause of diseases in both humans as well as animals. Consequently, any action that is associated with the production of livestock must be intimately explored to spot any vulnerability with liable Islamic laws of food and hit upon those traditions to avoid them. The intent of this review is to appraise the halal nutritional system of animals and see whether it has any optimistic or depressing effects on the health of the customers. Our advancement will be comprehensive as we have focused on the socio-religious aspects of human health. The verdict of this review could be made as an orientation by the policymakers of emerging officially authorized business bodies for producing, importing and auction of halal animal feed according to Islamic set of guidelines.
Diffuse cutaneous mastocytosis was diagnosed in a 6-year-old, indoor, neutered female domestic European shorthair cat. Marked pruritus located mainly on the head and neck was noticed in the cat and in this area the animal had developed alopecia, crusts, and plaques. Histologically, monomorphic mast cells were found in the superficial dermis and around the hair follicles. Mast cells were well differentiated, with central nuclei and granular cytoplasm, with metachromatic granules which stained positively with Toluidine blue stain. The animal was successfully treated with oclacitinib at a dose of 1 mg/kg, twice a day per os.
Since there is not enough data about milkability of the Jersey cows, the aim of this paper is to show basic milkability traits of this cattle breed depending on the parity and milk flow curve types. Cows had average daily production (DMY) of 22.23 kg, milk yield per milking (MYM) of 9.72 kg, and average and maximum milk flow about 1.66 and 2.49 kg/min, respectively. Statistical analysis showed that DMY and MYM of the cows in 4th parity was significantly (p---lt---0.05) higher comparing to cows in 1st and 3th parity. The duration of entirely milking is similar regarding the paritiesand only the cows in 3th and 4th differ significantly (p---lt---0.05). Regarding the effect of milk flow curve, the significant (p---lt---0.05) difference between unspecified and rectangular milk flow curve has been found for the average milk flow (AMF) and descending phase of the milk flow curve (TD). Cows with unspecified milk flow curve, have significantly (p---lt---0.05) lower AMF and longer TD compared to cows with rectangular curve. Cows with bimodal milk flow curve have significantly (p---lt---0.05) longer duration of the ascending phase compering to: unspecified, descending and rectangular. The results of this study have shown that Jersey cows have lower production and milk flow compared to other dairy cattle breeds. Nonetheless, they have uniform milkability traits, and a large representation of desirable milk flow curves which are associated with a beneficial effect on the udder health.