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Abstract

Objective: To determine the symptoms, associated pathologies and features of Childhood Celiac Disease in the population of eastern Algeria.

Study: The retrospective study was conducted in Pediatric Specialized Hospital of Sidi Mabrouk - Constantine. This study was based on data of 100 patients.

Results: In this sample, we noted a diversity of signs and associated pathology, a variety of digestive symptoms represented mainly by abdominal pain (59%), chronic diarrhea (51%) and vomiting (48%). Extra-digestive symptoms represented mainly by palorous skin and mucosa, delayed status and weight and stagnation of the weight with the percentages: 69%, 53% and 51% respectively. In addition, we noted biological disorders and a series associated pathologies namely: anemia (29%) and mouth ulcers (6%).

Conclusion: Childhood Celiac Disease (CD) of eastern Algeria can be expressed by a variety of digestive symptoms and gastrointestinal disorders (GI) in addition to a series of associated conditions.

Abstract

C. (Morphocarabus) rothi comptus Dejean 1831, is a endemic species of the Romania, appearing localized to some mountainous massifs of Romanian Banat and their piedmont areas (Poiana Rusca Mountains, Țarcu Mountains). Its presence in the Apuseni Mountains is doubtful, due to the lack of recent captures and to the confusion with C. (Morphocarabus) hampei diffinis Csiki 1905, morphologically like. In Banat the species occurs in two forms: szorenyensis Csiki 1908 at higher altitude (1300-2000 m) and ulrichhoffmanni Lie 1982 in hilly areas, which is distinguished by a larger size. Whatever the altitude and the ecological conditions, the period of activity is brief, spread over a month with a maximum of over two weeks. Molecular biology studies: confirm the belonging of comptus to rothi as a subspecies of her (28SRNA5 marker); suggest (concatenation COI I / cyt b) a low infra-specific variability between provenances of the szorenyensis (Rusca, Muntele Mic) and between the origins of the ulrichhoffmanni.

Abstract

Jojoba cultivation and production face the challenge of establishing ways to identify the sex at early stage of plant growth. The present study was carried out to identify sex of jojoba at the seedling stage under Sudan condition. Two DNA markers, ISSR (UBC807) and RAPD (OPG-5), were used for sex identification of jojoba genotypes: two known male and females genotypes and four unknown genotypes. ISSR marker, UBC807 was successfully amplified a unique male-specific band at 1200 bp, while RAPD marker, OPG-5 could not amplify a unique band within jojoba sex. The result clearly indicates that ISSR-UBC807 marker can be used for sex identification of jojoba at seedlings stage, a finding that could make the commercial cultivation and production of jojoba possible in Sudan.

Abstract

Digenetic trematode infections including schistosomiasis and fascioliasis have highly neglected statuses but are a menace to people in the poorest countries of the tropics, causing high morbidity and mortality in humans as well as great global losses in livestock production. This has neccesitated the widespread search for better control options for the snail vectors of these diseases. Hence, a novel drug - curcumin and nisin poly lactic acid (PLA) entrapped nanoparticles (CurNisNp) was screened for molluscicidal activity against the adults (> 2 months old) of Biomphalaria pfeifferi, Bulinus globosus and Lymnaea natalensis vector snails. Mortality was determined after 96-h of exposure at varying concentrations. The snails of the species L. natalensis were found to be the most susceptible to the molluscicide (LC50 323.6 ppm). This finding further supports the desirability of curcumin-nisin polylactic acid (PLA) nanoparticles as a molluscicide and therefore shows that it could be a good alternative to conventional molluscicides with prospects in the selective control of fascioliasis. However, more optimization of the drug could ensure a greater molluscicidal potency.

Abstract

Bread, which is a major breakfast food, has been documented to be susceptible to contamination by toxic fungi metabolites (mycotoxins) in various parts of the world. Total aflatoxins (AfT) and fumonisins (FB) are two important mycotoxins known for their ability to cause health damage to animals and humans when ingested through food over a long time. This study set out to determine the presence and level of these mycotoxins in bread produced and/or consumed within Lokoja, Kogi State, Nigeria. After extraction, ELISA was used to quantify the toxins in 30 samples of bread. The outcome showed 50% (15/30) contamination of bread samples by AfT and 93.3% (28/30) contamination by FB within the ranges 0.1-5.5 μg/kg and 10-220 μg/kg respectively. Three (3) of the bread samples were contaminated by AfT beyond the safe limit, while all the samples contaminated with FB were contaminated within the safe limit. The mutual occurrence of both toxins was recorded at 46.7%. EDI for AfT and FB were estimated to be 0.0048 μg/kg bw/day and 0.3379 μg/kg bw/day respectively while risk characterisation gave an estimated TDI% of 16.896% for FB. The results suggest that chronic toxic effects rather than acute toxicity could occur from long-term exposure to AfT and FB from bread. It is therefore advised that the raw materials used in bread manufacturing should be monitored and regulated for mycotoxins.

Abstract

Fungi have been associated with contamination and spoilage of food. These organisms are known to thrive in different weather and environmental conditions. Maize kernel obtained from field, store and market from 25 Local Government Areas of Niger State within the month of August to September were analyzed for fungi occurrence using standard methods. Ten different fungus; Aspergillus niger, A. ochraceus, A. flavus, A. fumigatus, A. parasiticus, Mucor spp, Fusarium spp, Penicillium spp, Yeast and Rhizopus spp, were identified and isolated from the maize kernel. From the agro-ecological zones of the state, the most predominant fungi were Aspergillus species (241/345), Rhizopus spp (41/345) and Mucor spp (37/345) while Penicilium spp and yeast were the least identified. Within the zones, Aspergillus species have the highest occurrence; wet (65/95), driest (63/95), wettest (56/95) and dry zone (54/95) zones. The highest occurrence of fungi was found in the market sample (129) followed by store (112) and field (104). High percentage occurrence of 11.6% was recorded in Suleja, Wushishi, and Borgu Local Government Area, followed by 10.6, 10.1, 8.7, 9.7, 9.2, 8.7 and 8.2% in Bosso, Tafa, Madalla, Minna, Paiko, Bida and Sabon wuse respectively. Mold species known to be mycotoxin producers were reported in this work. This poses as a risk factor to food safety.

Abstract

Diet has a major influence on both, human health and their behavior. Raw veganism is a diet that brings together two concepts: the one of veganism and the one of raw foodism, a diet that excludes all ASF (Animal Source Foods), all food cooked at a temperature above 48 °C (118 °F), and any food that is changed from its ordinary condition, and/or processed. The aim of the study was to bring more information about raw vegan diet and the controversy raw vegan versus cooked food. Scientific data taken into account recommend the use of the raw vegan diet mostly for a short period.

Abstract

Date palm is one of the most important economical crops in the world. Sex determination of date palm in early stage is a prerequisite for breeding and cultivation. The aim of this study is to validate RAPD and ISSR markers for sex identification of date palm genotypes grown under Sudan conditions. DNA was extracted from ten seedlings and five male and female plants using CTAB method. Eight primers, six RAPD and two ISSR primers were examined for their validation in sex determination of date palm genotypes. PCR amplification was performed using these primers. Four RAPD primers OPA02, OPJ-09, RD A02 and RD A21 were amplified male specific band with size of 1000, 1100, 1000 and 1400 pb respectively, while ISSR markers could not. The specific bands were observed clearly among all male genotypes and absent in female samples unknown samples irrespective of genotypes. Our results could be useful for sex determination of date palm sex in seedling stage and would promote date palm cultivation and production.