Browse

1 - 10 of 2,354 items :

  • Veterinary Medicine x
Clear All
Early detection of active glomerular lesions in dogs and cats using podocin

Abstract

In veterinary medicine, sensitive and specific markers of the early stages of renal failure still remain to be established. Podocytes could be a promising diagnostic tool in veterinary nephrology, especially in the differentiation of active pathological disease and glomerulopathies. Podocin is one of the robust proteins exploitable in detection of podocyturia. This article presents podocyte detection in urine for diagnostic purposes in veterinary medicine using a variety of methods. We describe the advantages and disadvantages of the immunohistochemical technique currently used, and of scanning microscopy, chromatography, and immunostaining. The identification of podocin-positive cells is a promising diagnostic tool in the detection of the early stages of glomerular basement membrane damage. The detection of renal failure prior to the occurrence of azotaemia is of high clinical importance from the clinical and scientific points of view.

Open access
Elaboration of triplex PCR for detection of selected viral infections in waterfowl

Abstract

Introduction

Viral infections are the greatest threat to waterfowl and cause significant economic losses. Diagnosis and differentiation of three goose viruses is difficult in the field and often requires laboratory confirmation. Therefore, the aim of the study was to develop a triplex PCR and optimise its parameters for simultaneous detection of DNA of goose parvovirus (GPV), goose polyomavirus (GHPV), and goose circovirus (GoCV).

Material and Methods

The DNA of viruses isolated from field cases from the National Veterinary Research Institute’s own collection was used for the study. The primer attachment temperature, the number of reaction cycles, and the Taq DNA polymerase and Mg2+ concentrations were optimised. The sensitivity and specificity of this triplex PCR was also determined.

Results

Based on the obtained results, triplex PCR parameters were optimised for simultaneous detection of DNA of GPV, GHPV, and GoCV in one sample. The following PCR products of the expected size were obtained: GPV DNA of 806 bp, GoCV DNA of 571 bp, and GHPV DNA of 180 bp.

Conclusion

The developed triplex PCR method proved to be useful for simultaneous detection of infections with three waterfowl viruses and will be used in relevant laboratory diagnostics.

Open access
Gastrointestinal nematodes of goats: host–parasite relationship differences in breeds at summer mountain pasture in northern Italy

Abstract

Introduction

The Orobic goat is a hardy breed native to the Orobic Alps (Lombardy, northern Italy). The aim of the study was the assessment of gastrointestinal nematode (GIN) egg excretion in Alpine and Saanen (cosmopolite breeds) and Orobic grazing goats, after a strategic treatment with eprinomectin in late June.

Material and Methods

Individual faecal samples from a mixed flock of cosmopolite and Orobic goats were collected and analysed by the FLOTAC double technique every three weeks from June to September.

Results

Strongylida was the primary GIN infection observed in goats that grazed on Alpine pastures; a strategic treatment with eprinomectin led to a prolonged reduction of egg excretion during the whole study period. Egg excretion was also influenced by breed. Pluriparous Orobic does were able to control reinfection better than the pluriparous cosmopolite does. Regarding Nematodirus sp. eggs per gram of faeces (EPG), the autochthonous Orobic breed presented higher values than the cosmopolite breeds. However, cosmopolite goats presented higher EPG values of Strongyloides papillosus than their Orobic counterparts in August.

Conclusions

Further studies on genetic features of local autochthonous goats, such as the Orobic breed, are needed, since they could reveal peculiar characteristics of susceptibility, resistance or resilience to GIN infection, providing genetic resources for selection.

Open access
Molecular detection and genetic diversity of porcine circovirus type 3 in commercial pig farms in Xinjiang province, China

Abstract

Introduction

Porcine circovirus type 3 (PCV3) is a newly discovered porcine circovirus. The molecular characteristics and genetic evolution of PCV3 in Xinjiang province, China still being unclear, the aim of the study was their elucidation.

Material and Methods

A total of 393 clinical samples were collected from pigs on commercial farms in nine different regions of Xinjiang and phylogenetic analysis based on full-length Cap genes was performed.

Results

The prevalence at farm level was 100%, while in all the tested samples it was 22.39%. Nine PCV3 strains were detected in Xinjiang province and they shared 98.9–99.3% nucleotide and 97.5–100.0% Cap gene amino acid sequence identities with other epidemic strains from China and abroad. Compared with other epidemic strains of PCV3, there were 26 base mutation sites in the Cap gene in the nine Xinjiang strains, resulting in the mutation of amino acids at positions 20, 24, 75, 77, 108, 111 and 206. Phylogenetic analysis showed that these strains can be divided into two different genetic groups, to the first of which five strains affiliated and divided between subgroups 1.1 and 1.2, and to the second of which the other four strains affiliated and similarly divided between subgroups 2.1 and 2.2.

Conclusion

PCV3 circulates widely among commercial pig farms in Xinjiang province, China, and displays obvious genetic diversity. The results provide epidemiological information useful for the prevention and control of PCV3 infection in the pig industry.

Open access
Pathological changes in natural infection of pheasants with highly pathogenic avian influenza A (H5N8) in Bulgaria

Abstract

Introduction

The study of histopathological changes caused by influenza A (H5N8) viral infection in bird species is essential for the understanding of their role in the spread of this highly infectious virus. However, there are few such studies under natural conditions in minor gallinaceous species. This article describes the pathomorphological findings in Colchis pheasants infected naturally with H5N8 during an epizootic outbreak in Bulgaria.

Material and Methods

Samples of internal organs of 10 carcasses were collected for histopathological and immunohistochemical evaluation, virus isolation and identification, and nucleic acid detection.

Results

Consistent macroscopic findings were lesions affecting the intestine, heart, lung, and pancreas. Congestion and mononuclear infiltrate were common findings in the small intestine, as were necrosis and lymphoid clusters in the lamina propria of the caeca. Congestion with small focal necrosis and gliosis with multifocal nonpurulent encephalitis were observed in the brain. Myocardial interstitial oedema and degenerative necrobiotic processes were also detected. Immunohistological analysis confirmed systemic infection and revealed influenza virus nucleoprotein in all analysed organs.

Conclusion

Variable necrosis was observed in the brain, liver, trachea, heart, small intestine, and caeca. Viral antigen was commonly found in the brain, heart, lung and trachea. Contact with migrating waterfowls was suspected as a reason for the outbreak.

Open access
Quality control of immunological veterinary medicinal products in Europe

Abstract

Medicinal products in Europe are under the strict control of many organisations headed by the European Directorate for the Quality of Medicines and HealthCare (EDQM) in Strasbourg and its related General European Official Medicines Control Laboratories (OMCLs) Network (GEON). The EDQM works in cooperation with the European Medicines Agency (EMA) and the World Health Organisation (WHO). All of these institutions have one main goal – to protect public health in Europe and around the world. One of the more important effects of the harmonisation of pharmaceutical law in Europe was the introduction of the mutual recognition principle for the Official Control Authority Batch Release (OCABR)/Official Batch Protocol Review (OBPR) certificates in the European Union. The National Veterinary Research Institute (NVRI) in Poland is an example of an OMCL laboratory within the Veterinary Batch Release Network (VBRN) that issues the European certificates. The NVRI is actively involved in the batch release of immunological veterinary medicinal products (IVMPs), with approximately 1,800 certificates for IVMPs issued per year. It is also one of only four veterinary OMCLs that perform Post Marketing Surveillance (PMS) studies including approximately 47 IVMPs per year. All the results of the testing data are sent to the Chief Veterinary Officer, and also to the electronic Network platforms of the EDQM, which enables transparent information exchange.

Open access
Seroprevalence of Q fever in sheep and goats from the Marmara region, Turkey

Abstract

Introduction

The aim of this study was to investigate Q fever seroprevalence in sheep and goats in the Marmara region. Q fever is a zoonotic disease caused by Coxiella burnetii. In ruminants, the disease causes reproductive disorders, premature births and stillbirths.

Material and Methods

Blood samples of sheep and goats were collected from the Marmara region of Turkey and a commercial ELISA was used for detection of specific antibodies to C. burnetii. A total of 832 samples (627 from sheep and 205 from goats) obtained from 126 herds located in 110 villages in 63 municipalities across all 11 provinces were utilised.

Results

Total seroprevalence was found to be 13.22%, while the proportion of seropositive herds was determined to be over threefold higher at 42.85%. The seroprevalence for sheep was found to be 14.19%, and for goats 10.24%. The herd seropositivity rate for sheep of 46.31% and for goats of 32.25% were also over threefold higher than the species-level seroprevalences. The provincial seroprevalence varied between 1.38% and 21.79%.

Conclusion

This study confirms the presence of C. burnetii in sheep and goat herds in the Marmara region and provides original seroprevalence data in hitherto uninvestigated provinces. The data gathered are beneficial for evaluation and elaboration of the seroprevalence of Q fever in sheep and goats in the Marmara region. Surveillance studies should be maintained, particularly in provinces with high seropositivity rates.

Open access
Tritrichomonas foetus as a causative agent of tritrichomonosis in different animal hosts

Abstract

Tritrichomonas foetus is a protozoan parasite that has been traditionally identified as a cause of reproductive tract disease in cattle and gastrointestinal tract infection in cats. Moreover, T. foetus is also well known as a commensal of the nasal cavity, intestines, and stomach in swine. In this review we describe T. foetus as a pathogen dangerous to more than one animal host, diagnostic and taxonomic aspects of this infection, and the extent to which isolates from different hosts share genetic identity.

Open access
Biochemical and haematological blood parameters of sows and piglets fed a diet with a dried fermented rapeseed meal

Abstract

Animal health and welfare can be assessed using biochemical and haematological markers of the blood. The values of these parameters depend in part on the quantity and quality of feed ingredients, i.e. feed protein and feed additives. The aim of the study was to determine the effect of including fermented rapeseed meal (FRSM) in dry feeding system on haematological and biochemical blood parameters of sows and piglets. The experimental material comprised 30 primiparous gilts and 30 multiparous sows after their second lactation. They were randomly divided into two groups of equal size – control and experimental. The animals in control groups CG (15 gilts) and CS (15 sows) received a standard diet for pregnant or lactating sows, depending on the reproductive period. Experimental groups EG and ES were 15 gilts and 15 multiparous sows, respectively, receiving feed with a 4% share of FRSM in place of soybean meal up to 100 d of gestation. In addition, from 100 d of gestation to 7 d of lactation, the sows in these groups received feed with a 9% share of FRSM, and then again a diet with a 4% share of FRSM until the end of lactation. Blood samples were taken from 6 animals from each group in two periods: at 100 days of pregnancy (late pregnancy) and at 27 days of lactation (late lactation). Blood from piglets was taken at 27 days of age (before weaning), from two piglets from each sow (one gilt and one barrow), taking into account the average body weight in the litter. Haematological parameters: Ht, Hb and RBC were determined in whole blood. The plasma content of minerals, activity of selected enzymes and biochemical parameters of sows, gilts and piglets were determined. The diet containing fermented rapeseed meal, fed to pregnant and lactating sows, increased the level of Ht and Hb and RBC content and mineral content (phosphorus, calcium and iron) in the plasma. This effect was mainly observed in primiparous sows. The inclusion of FRSM in the diet of sows reduced the plasma content of total cholesterol and triacylglycerols in sows and piglets, as well as liver enzyme activity, particularly AST in piglets. The use of fermented rapeseed meal in sow diet resulted in better use of mineral compounds, improvement of production effects and health parameters of sow and piglet blood.

Open access
The chemical composition of domestic soybean seeds and the effects of partial substitution of soybean meal by raw soybean seeds in the diet on pigs’ growth performance and pork quality (m. longissimus lumborum)

Abstract

The objective of the study was to determine the effect of the introduction of 5% of raw soybean seeds instead of soybean meal on the growth, feed consumption and utilization of growing pigs, also the carcass and pork quality. The growth experiment was conducted on 120 pigs of approx. 18.5 kg allocated to two dietary treatments. The animals from the control treatment (CON) were offered a diet with soybean meal, and the experimental group (EXP) was given 5% raw soybean seeds (NON-GMO, Augusta var.) instead of SBM. The experiment lasted 88 days. After the experiment, eight pigs from each group were euthanized and meat samples were collected. No diet effect on the animals’ performance and carcass quality were observed (P>0.05). The experimental diet affected (P<0.05) meat color, and also meat composition (higher water content and lower intramuscular fat content). The composition of fatty acids in the meat did not differ significantly, except for higher C16:1 content in the EXP group.

The introduction of 5% raw soybean seeds in the diets did not impact animal performance, but it reduced some indices of the pork quality.

Open access