The aim of this study was to investigate the effect of Radix Glehniae on the migration and invasion abilities of lung cancer cells.
Normal bronchial cell line 16HBE and lung cancer cell line SK-MES-1 were treated with Radix Glehniae extract. Proliferation, migration, and invasion abilities were determined by Cell Counting Kit (CCK)-8, Transwell, and Matrigel assays, respectively. The expression and secretion levels of tissue inhibitor of metalloproteinases 2 were detected by quantitative PCR and enzyme-linked immunosorbent assay, respectively.
Radix Glehniae extract inhibited the migration and invasion abilities of SK-MES-1 cells and enhanced TIMP2 expression and secretion by SK-MES-1 cells, without causing toxicity to 16HBE cells.
Radix Glehniae is useful in lung cancer treatment.
A liver fluke is a digenetic trematode parasitizing in the hepatic ducts of human beings or animals. Patients with liver fluke infection suffer from a series of hepatobiliary diseases. The prevalence of cholangiocarcinoma is significantly high in areas with a high incidence of clonorchiasis. A liver fluke is an important biocarcinogenic factor in the occurrence of cholangiocarcinoma. The secretory products of the body of this parasite and long-term mechanical stimulation induce continuous inflammation of the bile duct. Gene expression of the bile duct cells is imbalanced, leading to carcinogenesis of the bile duct. This article provides a summary of recent studies on the epidemiology, clinicopathology, and molecular biology of cholangiocarcinoma induced by liver fluke infection.
Recently, a new type of CD8+ T-cell subset, namely, the chemokine (C-X-C motif) receptor 5 (CXCR5+) cluster of differentiation (CD8+) T-cell subset (also called the follicular cytotoxic T-cell (TFC) subgroup), has been discovered around B-cell follicles. The discovery has aroused widespread interest. However, the processes and mechanisms of TFCs taking part in the immune response of the germinal center and their specific roles must still be clearly identified. This article reviews domestic and foreign studies on factors regulating the phenotype, physiological functions, maturity, and differentiation of TFCs and roles and clinical significance of these cells in HIV infection. This review has shown good application prospects for TFCs. The author believes that further studies on TFCs can provide another tool for cytotherapy to control or cure chronic viral infections or tumors.
The health status of the vaginal microenvironment, a complicated system, is an important indicator of female reproductive health. The vaginal flora is in a state of balance, and the microorganisms coexist and are interdependent to maintain the vaginal microecological balance, which is a kind of dynamic balance influenced by endogenous and exogenous factors. Vaginal infections are traditionally treated by killing microbes in the vagina. Given the extensive study of the internal vaginal environment, people have become gradually aware of the significance of maintaining the vaginal microecological balance rather than blindly using antimicrobial agents. The balance in the vaginal internal environment is disrupted during the gestation period as the secretion of progesterone increases. The imbalanced vaginal microecological environment may lead to vaginal infectious diseases. This article provides a review of the relationship between the vaginal microecology and infectious diseases during the gestation period.
Recipients with a low immunity are under a high risk of infection due to the extensive use of immunosuppressive drugs after renal transplantation. Pulmonary infection after renal transplantation is a prevalent postoperative complication characterized by a wide range of pathogens and high mortality. If the disease cannot be diagnosed in time, then the therapeutic effect will not be effective. This article reviews susceptible factors, high onset time, common pathogens, clinical manifestations, and therapy of pulmonary infection after renal transplantation to provide reference for disease prevention and treatment.
Scavenger receptor class B type I (SR-BI) is a high-affinity receptor for high-density lipoprotein (HDL). The primary role of this receptor is the selective uptake of HDLs in the liver through reverse cholesterol transport. SR-BI interacts with HDL to regulate lipid metabolism and affects various vascular cell functions involved in atherosclerosis (As). In addition, SR-BI is involved in the development of malignant tumors and infectious diseases. This article reviews the function and potential therapeutic targets of SR-BI in As, malignancies, and infectious diseases.
Zhi-Jian Li, Xing-Ling Sui, Xue-Bo Yang and Wen Sun
To reveal the biology of AML, we compared gene-expression profiles between normal hematopoietic cells from 38 healthy donors and leukemic blasts (LBs) from 26 AML patients. We defined the comparison of LB and unselected BM as experiment 1, LB and CD34+ isolated from BM as experiment 2, LB and unselected PB as experiment 3, and LB and CD34+ isolated from PB as experiment 4. Then, protein–protein interaction network of DEGs was constructed to identify critical genes. Regulatory impact factors were used to identify critical transcription factors from the differential co-expression network constructed via reanalyzing the microarray profile from the perspective of differential co-expression. Gene ontology enrichment was performed to extract biological meaning. The comparison among the number of DEGs obtained in four experiments showed that cells did not tend to differentiation and CD34+ was more similar to cancer stem cells. Based on the results of protein–protein interaction network, CREBBP, F2RL1, MCM2, and TP53 were respectively the key genes in experiments 1, 2, 3, and 4. From gene ontology analysis, we found that immune response was the most common one in four stages. Our results might provide a platform for determining the pathology and therapy of AML.
Oral lesions are highly correlated with the occurrence and development of many diseases. In addition, the treatment of systemic diseases may aggravate oral focal infections, affect the life quality of patients, interfere with the treatment of systemic diseases, and even cause systemic infection in serious cases. Treatment strategies for systemic diseases may induce or aggravate oral local lesion infections. In specific, administration of oral antiepileptic drugs and immunosuppressive drugs may induce gingivitis, radiotherapy or chemotherapy for malignant tumors may cause oral mucositis, long-term use of bisphosphonates for inhibition of tumor bone metastasis or prevention of osteoporosis may cause osteonecrosis of the jaw, and allogeneic hematopoietic stem cell transplantation may cause oral rejection reactions.
Wang Zhen-fei, Mu Yong-ping, Liang Jun-qing, Liu Yong-yan and Li Jing-quan
This study aimed to investigate the influence of Xanthii fructus on the expression of small noncoding RNA (sncRNA) and the malignant behaviors of lung cancer cells.
A549 cells were treated with Xanthii fructus extract. SncRNA expression was detected by real-time PCR. Proliferation, anchorage-independent growth, and invasion capacities were determined using Cell Counting Kit (CCK)-8, soft agar colony formation, and Matrigel assays, respectively.
Xanthii fructus extract downregulated microRNA (miR)-21 expression and upregulated PIWI-interacting RNA (piRNA)55490 expression. The proliferation, anchorage-independent growth, and invasion capacities of A549 cells were strongly inhibited by the extract.
Xanthii fructus can inhibit the malignant behaviors of lung cancer cells.
As one of the main factors affecting safe blood transfusion, hepatitis B virus (HBV) infection through blood transfer seriously endangers human health. Therefore, studies should focus on both reducing infection rate of HBV and accurately evaluating the risk of infection. This study discusses the main factors affecting HBV infection that results from blood transfusions, with the aim of gaining insights into reducing HBV infection.