Nikolay Uzunov, Michele Bello, Laura Melendez-Alafort and Laura De Nardo
In recent years, there is a constantly increasing interest in the application of nanoparticles for cancer diagnosis and cancer therapy. In this respect, the most promising nano-objects at present are the gold nanoparticles. A very convenient and powerful property of these objects is their ability to increase their temperature under electro-magnetic irradiation with certain wavelength. In our research we have directed our efforts toward particular nano-objects specifically sensitive to electromagnetic radiation in the near-infrared region (NIR). In order to study the photothermic properties of the solutions of gold nanoparticles in the NIR we constructed a specific electronic setup consisting of a laser system with interchangeable laser diodes with different wavelength NIR light, a thermally-insulated cuvette-holder compartment with temperature measuring probes and a NIR spectrometer to control the stimulated fluorescence emission of the nanoparticles’ solutions. The temperature measurement compartment with the thermal-insulated cuvette holder was designed to maintain the solutions’ temperature at a fixed value right before the moment of laser irradiation. To maintain the measurement setup at a fixed temperature before the irradiation we used a thermal stabilized system based on two Peltier cells with electronic temperature control. The temperatures of the ambient air and the temperature of the cuvette walls were continuously measured in order to make corrections about the temperature dissipation during the irradiation.
Djamila Maanani, Narimane Segueni, Salah Rhouati, Yavuz Selim Çakmak, Maltem Asan-Ozusaglam, Abdghani May, Amar Zellagui and Salah Akkal
This study was carried out to determine the phenolic contents as well as to evaluate the antioxidant and antimicrobial activities of Genista microcephala. Total phenolic and flavonoid compounds were quantified. Antioxidant activity was evaluated by various in vitro tests, including Ferric Ion Reducing Activity (FRP), Cuprac Ion Reducing Antioxidant Capacity (CUPRAC), and total antioxidant capacities (TAC). The antimicrobial activity of ethyl acetate and butanolic extracts of G. microcephala in addition of fatty acids content of different parts of the studied plant was evaluated against human pathogenic bacteria, fish bacteria and two yeast. The used methods were agar diffusion method and the determination of minimal bactericidal and fungicidal concentrations (MBC and MFC). No significant difference was found between the two used solvents on total phenolic content (TPC). While, difference in total flavonoid content (TFC) were found to be significant. Ethylene acetate extract was the most actif regarding antioxidant assay. The tested activity seems to be due to the high content of this extract on isoflavonoids. The tested extracts demonstrated the lowest inhibition concentration for B.cereus RSKK 863.
The objective of the present work is to prepare and characterize a porous material using quite particular and localized dunar sand (erg) of the desert near the El-Oued (south-east of Algeria). The porous material is prepared according to the mechanism of co-operative self-assembly. The method consists of polymerizing a silicic precursor (sodium silicate obtained by alkali fusion of sand with the sodium carbonate) around micelles of surfactant in an acid aqueous solution according to the sol-gel process. The elimination of the surfactant by calcining at high temperature leads to the final material which was characterized by XRF, XRD, MEB-EDX, FTIR and BET techniques.
Iroanya Onyekachi Ogbonnaya, Egwuatu Tochukwu Frank, Sonaike Adeola Olasubomi and Uwaezuoke Nduka David
Identification of dismembered bodies recovered in disasters or crime scene is very important in forensic. This study was carried out to ascertain if foot size, body weight, height, stature and BMI can be used to predict sex using tape rule and Stadiometer scale. 150 subjects between 18 - 25yrs were recruited. Independent T-test, Pearson Correlation, Linear and Multiple Regression Analysis were determined using SPSS version 23. All measurements were higher in male than female and there was significant (p≤0.05) difference in the sex. The average foot length of 257.39mm was used to determine sex of the subjects. All foot lengths ≤ 257.39mm were presumed to belong to females while all values > 257.39mm was presumed males. Foot size and weight had the highest correlation values. Foot length gave better estimation of sex than foot breadth, BMI and weight. Results of Multiple regression equation was better than linear regression equation. In forensic anthropology, foot size has been used to predict sex. Limitation of this study is the formulae generated can only be applied to Nigerians aged 18-25 yrs. Studies should be carried out on diverse population for future inferences
P. Petkova, P. Vasilev, Gulsah Celik Gul, M. Mustafa and I. Parushev
The absorption coefficient of Bi12TiO20:AlI, Bi12TiO20:AlII, Bi12SiO20:P and Bi12SiO20:Al+P single crystals is measured in the spectral region of Urbach’s rule (1.52 – 2.92 eV) at room temperature. The parameters of electron-phonon interaction, Urbach’s energy and the constants of Urbach’s rule are calculated. The behavior of the acceptors Al3+ and P5+ in Urbach’s rule region has been considered.
The reactivity of the titled compounds towards electrophilic reagents has been investigated and new experimental evidences for the mechanism of the electrophilic addition reactions to 1,2-alkadienephosphonates have been reported
This study continues the attempt to use the statistical process for a large-scale analytical data. A group of 3898 white wines, each with 11 analytical laboratory benchmarks was analyzed by a fingerprint similarity search in order to be grouped into separate clusters. A characterization of the wine’s quality in each individual cluster was carried out according to individual laboratory parameters.
Simeon Lukanov, Georgi Popgeorgiev and Nikolay Tzankov
Water frog mating calls from two localities were studied and analyzed. Recordings were made in the summer of 2010 at the Arkutino swamp near the town of Primorsko and at the Vurbitza River near the town of Momchilgrad. A total of 154 calls were analyzed and the results suggested the presence of both the Marsh frog (Pelophylax ridibundus) and the Levant frog (Pelophylax bedriagae) in both sites, with the former being more frequent in Vurbitza River, and the latter – in Arkutino. At Vurbitza, we also captured and measured 2 specimens, which morphological characteristics differed from P. ridibundus and matched those of P. bedriagae. These are the first localities for P. bedriagae in Bulgaria.
Vanya Koleva, Asya Dragoeva, Zheni Stoyanova, Zhenia Yordanova, Selime Ali, Nikolay M. Uzunov, Laura Melendez-Alafort, Antonio Rosato and Dobromir D. Enchev
Medicinal plants produce various secondary metabolites as a part of their chemical defence and survival in nature. These compounds have a wide range of biological activities. Nowadays, medicinal plants are used as source of allelochemicals and new effective anticancer agents. Our previous studies revealed allelopathic potential of water extracts of Adonis vernalis L. (Ranunculaceae), Origanum vulgare ssp. vulgare L. and Nepeta nuda subsp. nuda (Lamiaceae). Present study aimed to evaluate the effect of the same extracts in vitro on human hepatoma cell line SK-HEP-1. Cell proliferation/viability was assessed using Premixed WST-1 Cell Proliferation Reagent.
Adonis water extract (1.83mg/ml) had notable negative influence on cancer cell line tested. Oregano (3.5 mg/ml) also exerted negative effect, but to a lesser degree. On the contrary, nepeta water extract (6.59 mg/ml) had an opposite effect, stimulating cell proliferation. One possible explanation could be the type of extraction: after treatment with nepeta methanol extract (6.59 mg/ml) cell viability was significantly reduced.
In conclusion, Adonis vernalis and Nepeta nuda subsp. nuda possess metabolites with growth inhibitory effect on human hepatoma cell line SK-HEP-1. Further research is needed to clarify biological activity of lower concentrations which are appropriate to enable the design of new anticancer drugs.