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Valdis Pirsko, Inese Čakstiņa, Dina Nitiša, Marija Samoviča, Zanda Daneberga and Edvīns Miklaševičs

Abstract

Development of chemoresistance remains a significant limitation for the treatment of cancer and contributes to recurrence of the disease. Both intrinsic and acquired mechanisms of chemoresistance are characteristics of cancer stem cells (CSCs) or stem-like cells (SLCs). The aim of the study was to assess the stem-like properties in the breast cancer cell line MDA-MB-231 during and after pulsed treatment with doxorubicin (DOX) in comparison to the untreated controls.The experimental cultures were exposed to therapeutic concentration of DOX for 48 hours (treatment cultures), and subcultured to post-treatment cultures 24 hours after the removal of DOX. Stem-like properties of the cellular populations in the treatment and post--treatment cultures were assessed by the expression of the stem-cell marker genes (CD24, CD44, ITGA6, ITGB1, POU5F1, NANOG, ALDH1A1), colony-formation efficiency, growth rates, and sensitivity to DOX, 5-fluorouracil (5FU), cisplatin (CIS), and vinblastine (VBL). Exposure to DOX induced formation of giant polyploid cells that persisted in the post-treatment culture. The recovery period was characterised by a decrease in the proliferation rate, viability, and cellular adherence. The post-treatment cultures displayed decreased sensitivity to DOX and increased sensitivities to 5FU, CIS, and VBL. Cells treated with DOX displayed increased expression levels of CD24, CD44, and ALDH1A, while their expression levels at least partially normalised in the post-treatment culture. The post-treatment cultures demonstrated significantly increased colony-formation ability. During treatment with sub-lethal levels of doxorubicin and during the acute recovery period, the survival mechanisms in the breast cancer cell line MDA-MB-231 may be mediated by formation of the cellular population with stem-like properties.

Open access

Brahim Benmedjdoub, Isma Bouchemakh and Éric Sopena

Abstract

A 2-distance k-coloring of a graph G is a mapping from V (G) to the set of colors {1,. . ., k} such that every two vertices at distance at most 2 receive distinct colors. The 2-distance chromatic number χ 2(G) of G is then the smallest k for which G admits a 2-distance k-coloring. For any finite set of positive integers D = {d 1, . . ., d}, the integer distance graph G = G(D) is the infinite graph defined by V (G) = ℤ and uvE(G) if and only if |vu| ∈ D. We study the 2-distance chromatic number of integer distance graphs for several types of sets D. In each case, we provide exact values or upper bounds on this parameter and characterize those graphs G(D) with χ2(G(D)) = ∆(G(D)) + 1.

Open access

Robert A. Beeler, Teresa W. Haynes and Kyle Murphy

Abstract

Let G be a graph with vertex set V and a distribution of pebbles on the vertices of V. A pebbling move consists of removing two pebbles from a vertex and placing one pebble on a neighboring vertex, and a rubbling move consists of removing a pebble from each of two neighbors of a vertex v and placing a pebble on v. We seek an initial placement of a minimum total number of pebbles on the vertices in V, so that no vertex receives more than one pebble and for any given vertex vV, it is possible, by a sequence of pebbling and rubbling moves, to move at least one pebble to v. This minimum number of pebbles is the 1-restricted optimal rubbling number. We determine the 1-restricted optimal rubbling numbers for Cartesian products. We also present bounds on the 1-restricted optimal rubbling number.

Open access

Yuval

Studies in Jewish Music