An Experimental Study on the Mechanisms of Ge Hua Jie Cheng Decoction for Rats with Alcoholic Liver Injuries

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Objective: To explore the effects and underlying mechanisms of Ge Hua Jie Cheng Decoction on rats with alcoholic liver injuries.

Methods: 60 Wistar rats were randomly assigned to six groups: normal group, model group, Yi Gan Ling group, and Ge Hua Jie Cheng Decoction groups in low, middle and high concentrations, 10 rats in each group. Except for the normal group, rats in other groups were administered white wine for eight weeks to establish the liver injury model. During the modeling, the Yi Gan Ling/Ge Hua Jie Cheng Decoction were administered intragastrically to the rats. So the histopathological changes were observed after eight weeks, meanwhile the serum γ- glutamyl endopeptidase (GGT), Glutathione (GSH) and aspartate aminotransferase mitochondrial isoenzyme (m-AST) were assayed by automatic biochemical analyzer.

Results: under the light microscope, the groups of high and middle dosages of Ge Hua Jie Cheng Decoction , especially the high one, had apparent improvement of inflammatory infiltration in liver tissues. Compared with the normal group, the serum GGT and m-AST levels had elevated (P<0.01), whereas the serum GSH level decreased (P<0.01); compared with model group, the high and middle dosages of Ge Hua Jie Cheng Decoction groups had decreased serum GGT and m-AST (P<0.01 or P<0.05), as well as increased serum GSH level (P<0.01).

Conclusion: Ge Hua Jie Cheng Decoction has a protective effect for liver injuries induced by alcohol, and this effect is dose-dependent. The high dosage showed stronger protection effect, which might be related to the increased serum GSH and decreased serum GGT and m-AST.

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