An Experimental Study on the Mechanisms of Ge Hua Jie Cheng Decoction for Rats with Alcoholic Liver Injuries

Open access

Abstract

Objective: To explore the effects and underlying mechanisms of Ge Hua Jie Cheng Decoction on rats with alcoholic liver injuries.

Methods: 60 Wistar rats were randomly assigned to six groups: normal group, model group, Yi Gan Ling group, and Ge Hua Jie Cheng Decoction groups in low, middle and high concentrations, 10 rats in each group. Except for the normal group, rats in other groups were administered white wine for eight weeks to establish the liver injury model. During the modeling, the Yi Gan Ling/Ge Hua Jie Cheng Decoction were administered intragastrically to the rats. So the histopathological changes were observed after eight weeks, meanwhile the serum γ- glutamyl endopeptidase (GGT), Glutathione (GSH) and aspartate aminotransferase mitochondrial isoenzyme (m-AST) were assayed by automatic biochemical analyzer.

Results: under the light microscope, the groups of high and middle dosages of Ge Hua Jie Cheng Decoction , especially the high one, had apparent improvement of inflammatory infiltration in liver tissues. Compared with the normal group, the serum GGT and m-AST levels had elevated (P<0.01), whereas the serum GSH level decreased (P<0.01); compared with model group, the high and middle dosages of Ge Hua Jie Cheng Decoction groups had decreased serum GGT and m-AST (P<0.01 or P<0.05), as well as increased serum GSH level (P<0.01).

Conclusion: Ge Hua Jie Cheng Decoction has a protective effect for liver injuries induced by alcohol, and this effect is dose-dependent. The high dosage showed stronger protection effect, which might be related to the increased serum GSH and decreased serum GGT and m-AST.

1. You-Ming LI. Epidemiology and history of ALD. Chinese Journal of Hepatology 2010,18(3):171-172.

2. Wan-Zhi YANG. Research advancement of pathogenesis of liver diseases. Acta Universitatis Medicinalis Anhui 2012,47(1): 124-126.

3. Wei ZHANG, Ru-Tao HONG, Tu-Lei TIAN. Establishment of rat ALD models. Anhui Medical and Pharmaceutical Journal 2012, 16 (7):885-887.

4. Hua Jing. TCM etiology and pathogenesis of ALD. Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases 2012,22(4):249-250.

5. Chun-Hua WANG, Zhi-Lan YANG, Shu-Yun DUAN, et al. Clinical Investigation on Intervened Course of Acute Sever Alcoholism with Infusion of Jiaweighuajiechentang into Stomach.

6 Guo-Hui AN. Modified Ge Hua Jie Cheng Decoction for 46 cases of alcoholic liver disease. Chinese Medicine Modern Distance Education of China 2010,8(9):23-24.

7 Zhong-Cheng CHEN, Wen-Si CHEN. Clinical significance of GGT, AFP, CA199 in patients with liver diseases. Clinical Medical Engineering 2012,19(11):1903-1906.

8 Zong-Xiao DU, Fu-Rong LI, Wen-Hua PIAO, et al. Cllinical significance of serum AST in alcoholic liver disease. Laboratory Medicine,2012,27(9):732-735.

9 Ping QIU, Xiang LI, De-Song KONG, et al. New advaancement of research on alcoholic liver disease. Chinese Pharmacological Bulletin 2014,30(2):160-163.

10 Hong-Qiu YE, Du-Xin QING. Research progress on immune mechanisms of alcoholic liver disease. International Journal of Digestive Diseases 2011,31(5):283-285.

11 Ping-Ge YUAN, Da-Zhi ZHANG. Effect mechanism and clinical utilization of Glutathione. Drug Evaluation 2006,3(5): 385-388.

12 Xing-Xing SONG, Jin-Ling SU, Ye-Dong BI, et al. Experimental study on the effects of Wu Tian Bao Gan Ye on tissue SOD, MDA and GSH-PX in rats with alcoholic liver disease. Jilin Journal of Traditional Chinese Medicine 2011,31,(5):477-479.

Journal Information

Metrics

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 384 291 5
PDF Downloads 119 109 6