Short Note: Development and characterization of 16 new polymorphic microsatellite loci for Schima superba (Theaceae)

Open access


Schima superba is a common dominant tree species in evergreen broad-leaved forest in subtropical China. Despite its multiple usages in wood industry, reforestation and traditional Chinese medicine, its genetic diversity is poorly studied. To help studying its genetic diversity and structure in the future, after microsatellite enrichment and screening, we identified 16 microsatellites in S. superba. These markers showed polymorphism in three populations. The number of alleles per locus ranged from 3 to 32 with a mean of 14. Within populations, the observed and unbiased expected heterozygosities ranged from 0.048 to 0.926 and from 0.048 to 0.949, respectively. The newly developed 16 microsatellites will be useful for investigating the genetic diversity and structure from large scale patterns to fine-scale structures in this species.

EPPERSON, B. K. (1992): Spatial structure of genetic-variation within populations of forest trees. New Forests 42: 257-278.

HOLM, S. (1979): A simple sequentially rejective multiple test procedure. Scandinavian Journal of Statistics 6: 65-70.

LI, P., Z. L. HUANG, Y. C. XIANG and H. REN (2011): Survival, growth and biomass of Acacia auriculiformis and Schima superba seedlings in different forest restoration phases in Nan’ao island, south China. Journal of Tropical Forest Science 23: 177-186.

LUNT, D. H., W. F. HUTCHINSON and G. R. CARVALHO (1999): An efficient method for PCR-based isolation of microsatellite arrays (PIMA). Molecular Ecology 8: 891-894.

PEAKALL, R. and P. E. SMOUSE (2006): GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes 6: 288-295.

R DEVELOPMENT CORE TEAM (2011): R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051- 07-0, URL

RAYMOND, M. and F. ROUSSET (1995): Genepop (version 1.2): population genetics software for exact tests and ecumenicism. Journal of Heredity 86: 248-249.

WANG, X. H., X. H. MA, G. Q. JIN, L. Y. CHEN and Z. C. ZHOU (2011): Variation pattern of individual types and wood characters in natural stands of Schima superba. Scientia Silvae Sinicae 47: 133-139.

XU, W., H. WANG, G. X. ZHOU and X. S. YAO (2010): Two new 8-O-4’-type lignans from the stem of Schima superba and their cell growth inhibitory activities against human cancer cell lines. Journal of Asian Natural Products Research 12: 874-878.

YANG, S. X., H. DENG and M. S. LI (2008): Manganese uptake and accumulation in a woody hyperaccumulator, Schima superba. Plant Soil and Environment 54: 441-446.

ZHANG, P., G. Q. JIN, Z. C. ZHOU, L. YU and H. H. FAN (2004): Provenance difference and geographic variation pattern for seedling trait of Schima superba. Forest Research 17: 192-198.

Journal Information

IMPACT FACTOR 2017: 0.277
5-year IMPACT FACTOR: 0.455

SCImago Journal Rank (SJR) 2017: 0.225
Source Normalized Impact per Paper (SNIP) 2017: 0.382

Cited By


All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 128 125 8
PDF Downloads 46 46 4