The efficiency of sodC gene / N. meningitidis detection in comparison with the classical methods for the diagnosis of meningococcal infection / Evaluarea eficienţei Real Time PCR TaqMan utilizând gena sodC / N. meningitidis în comparaţie cu metodele clasice utilizate în diagnosticul infecţiei meningococice

Open access


Meningococcal infection requires a fast and accurate diagnostic method in order to correctly initiate the antibiotic therapy. The aim of our study was to assess the efficiency of Real Time PCR -Taq Man using sod C gene / N. meningitidis in comparison with the classical methods for the diagnosis of meningococcal infection - direct microscopy, cultivation, latex agglutination and blood culture. We have detected 24/44 (54.54%) patients with meningococcal infection. In both cases of patients with / without previous antibiotic therapy before admission, the AUC (area under curve) had the highest values for RT PCR in CSF and blood analysis. This sod C RT-PCR assay is a highly sensitive and specific method for detection of Neisseria meningitis and it would be useful to include this method like a multiplex in routine testing of patients with clinical meningococcal infection for other etiological agents also.

1. Strelow VL, Vidal JE. Invasive meningococcal disease. Arq Neuropsiquiatr. 2013 Sep;71(9B):653-8. DOI: 10.1590/0004-282X20130144

2. Qurbanalizadegan M, Ranjbar R, Ataee R, Hajia M, Goodarzi Z, Farshads et al. Specific PCR Assay for Rapid and Direct Detection of Neisseria meningitidis in Cerebrospinal Fluid Specimens. Iran J Public Health. 2010;39(4):45-50.

3. Higa FT, Fukasawa LO, Gonçalves MG, Salgado MM, Lemos AP, Harrison LH, et al. Use of sod C versus ctr A for real-time polymerase chain reaction-based detection of Neisseria meningitidis in sterile body fluids. MemInst Oswaldo Cruz. 2013 Apr;108(2):246-7. DOI: 10.1590/0074-0276108022013020

4. Dolan Thomas J, Hatcher CP, Satterfield DA, Theodore MJ, Bach MC, Linscott KB, et al. Sod C-based real-time PCR for detection of Neisseria meningitidis. PLoS One. 2011 May 5;6(5):e19361. DOI: 10.1371/ journal.pone.0019361

5. Abdeldaim GM, Strålin K, Korsgaard J, Blomberg J, Welinder-Olsson C, Herrmann B. Multiplex quantitative PCR for detection of lower respiratory tract infection and meningitis caused by Streptococcus pneumoniae, Haemophilus influenzae and Neisseria meningitidis. BMC Microbiol. 2010 Dec 3;10:310. DOI: 10.1186/1471-2180-10-310

6. Azzari C, Canessa C, Lippi F, Moriondo M, Indolfi G, Nieddu F, et al. Distribution of invasive meningococcal B disease in Italian pediatric population: implications for vaccination timing. Vaccine. 2014 Feb 26;32(10):1187-91. DOI: 10.1016/j.vaccine.2013.09.055

7. Stuart JM, Monk PN, Lewis DA, Constantine C, Kaczmarski EB, Cartwright KA. Management of clusters of meningococcal disease. PHIS Meningococcus Working Group and Public Health Medicine Environmental Group. Commun Dis Rep CDR Rev. 1997 Jan 10;7(1):R3-5.

8. CMIT. Infections a meningocoque. In E. Pilly: Vivactis Plus Ed; 2010:258-62.

9. Brouwer MC, Tunkel AR, van de Beek D. Epidemiology, diagnosis, and antimicrobial treatment of acute bacterial meningitis. Clin Microbiol Rev. 2010 Jul;23(3):467-92. DOI: 10.1128/CMR.00070-09

10. Buiuc D., Examenul lichidului cefalorahidian în diagnosticul infecțiilor sistemului nervos central, Tratat de Microbiologie Clinică, Ediția a II-a, Editura Medicală. 2008:182-95.

11. Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, KubistaM et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem.2009Apr;55(4):611-22. DOI: 10.1373/clinchem.2008.112797

12. Boiculese L, Dimitriu G, Moscalu M, PIM editor. Calitatea examenului diagnostic, Elemente de biostatistică, Analiza statistică a datelor biologice, Ed. PIM. 2007:183-9.

13. Branco RG, Amoretti CF, Tasker RC. Meningococcal disease and meningitis. J Pediatr (Rio J). 2007 May;83(2 Suppl):S46-53. DOI: 10.2223/JPED.1612

14. Stephens DS. Conquering the meningococcus. FEMS Microbiol Rev. 2007 Jan;31(1):3-14. DOI: 10.1111/j.1574-6976.2006.00051.x

15. Jolly K,Stewart G. Epidemiology and diagnosis of meningitis: results of a five-year prospective, population- based study. Commun Dis Public Health. 2001 Jun;4(2):124-9.

16. Carrol ED, Thomson AP, Riordan FA, Fellick JM, Shears P, Sills JA, et al. Increasing microbiological confirmation and changing epidemiology of meningococcal disease on Merseyside, England. Clin Microbiol Infect. 2000 May;6(5):259-62. DOI: 10.1046/j.1469-0691.2000.00078.x

17. Cartwright K, Reilly S, White D, Stuart J. Early treatment with parenteral penicillin in meningococcal disease. BMJ. 1992 Jul 18;305(6846):143-7. DOI: 10.1136/bmj.305.6846.143

18. Nemescu RE, Iancu LS, Dorneanu OS, Ursu RG, Dorobăţ CM. Influence of antibiotic therapy prior to admission on the efficacy of classical methods for the diagnosis of meningococcal disease. Rev Med ChirSoc Med Nat Iasi. 2014 Apr-Jun;118(2):497-502.

19. Gray SJ, Sobanski MA, Kaczmarski EB, Guiver M, Marsh WJ, Borrow R, et al. Ultrasound-enhanced latex immunoagglutination and PCR as complementary methods for non-culture-based confirmation of meningococcal disease. J ClinMicrobiol. 1999 Jun; 37(6):1797-801.

20. Ghotaslou R, Farajnia S, Yeganeh F, Abdoli-Oskouei S, Ahangarzadeh Rezaee M, Barzegar M. Detection of acute childhood meningitis by PCR, culture and agglutination tests in Tabriz, Iran. Acta Med Iran. 2012;50(3):192-6.

21. Saunders NB, Shoemaker DR, Brandt BL, Zollinger WD. Confirmation of suspicious cases of meningococcal meningitis by PCR and enzyme-linked immunosorbent assay. J ClinMicrobiol. 1997 Dec;35(12):3215-9.

22. Sacchi CT, Fukasawa LO, Gonçalves MG, Salgado MM, Shutt KA, Carvalhanas TR, et al. Incorporation of real-time PCR into routine public health surveillance of culture negative bacterial meningitis in São Paulo, Brazil. PLoS One. 2011;6(6):e20675. DOI: 10.1371/ journal.pone.0020675

23. Hackett SJ, Carrol ED, Guiver M, Marsh J, Sills JA, Thomson AP et al. Improved case confirmation inmeningococcal disease with whole blood Taqman PCR, Arch Dis Child. 2002 Jun;86(6):449-52. DOI: 10.1136/ adc.86.6.449

24. Bryant PA, Li HY, ZaiaA, GriffithJ, HoggG, Curtis N, et al. Prospective study of a real-time PCR that is highly sensitive, specific, and clinically useful for diagnosis of meningococcal disease in children. J Clin- Microbiol. 2004 Jul;42(7):2919-25. DOI: 10.1128/ JCM.42.7.2919-2925.2004

25. Guiver M, Borrow R, Marsh J, Gray SJ, Kaczmarski EB, Howells D, et al. Evaluation of the Applied Biosystems automated Taqman polymerase chain reaction system for the detection of meningococcal DNA. FEMS Immunol Med Microbiol. 2000 Jun; 28(2):173-9. DOI: 10.1111/j.1574-695X.2000.tb01473.x

Revista Romana de Medicina de Laborator

Romanian Journal of Laboratory Medicine

Journal Information

IMPACT FACTOR 2017: 0.400
5-year IMPACT FACTOR: 0.320

CiteScore 2017: 0.31

SCImago Journal Rank (SJR) 2017: 0.144
Source Normalized Impact per Paper (SNIP) 2017: 0.195


All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 179 179 17
PDF Downloads 68 68 5