Listeria monocytogenes has a ubiquitous distribution in nature and could contaminate food of animal origin, causing severe infections in humans. Till present, little is known about the antibiotic resistance profiles of these strains in Romania. The aim of this study was to determine the antibiotic susceptibility patterns of 37 L. monocytogenes strains isolated from animal derived foods and from clinical samples. Food samples were collected from meat and dairy products, between 2009 and 2013. Clinical samples were collected from patients with septicemia, meningitis/meningo-encephalitis, abortion cases and newborns, hospitalized during April 2010 - April 2013 in three medical institutions from Bucharest: Babes Hospital, Elias Hospital, National Institute of Infectious Diseases (INBI) Matei Bals. All tested isolates exhibited resistance to cephalosporins and nalidixic acid; one strain isolated from boiled shell snails was resistant to trimethoprim/sulfamethoxazole. The resistance to the first choice antibiotic ampicillin in L. monocytogenes strains isolated from severe infections is underlining the need of in vitro antibiotic susceptibility testing of each clinical isolate to establish the efficacy of different antibiotics, as well as of extended epidemiological studies to highlight the resistance profiles of L. monocytogenes strains circulating in our country.
1. Liu D. Identification, subtyping and virulence determination of Listeria monocytogenes, an important foodborne pathogen. J Med Microbiol. 2006 June;55(6):645-9. DOI: 10.1099/jmm.0.46495-0
2. Low JC, Donachie W A review of Listeria monocytogenes and listeriosis. Vet J. 1997 Jan; 153(1):9-29. DOI: 10.1016/S1090-0233(97)80005-6
3. Liu D, Lawrence M, Austin FW, Ainsworth AJ. Comparative assessment of acid, alkali and salt tolerance in Listeria monocytogenes virulent and avirulent strains. FEMS Microbiol Lett. 2005 Feb;243(2):373-8. DOI: 10.1016/j.femsle.2004.12.025
4. Sleator RD, Gahan CGM, Hill CA. postgenomic appraisal of osmotolerance in Listeria monocytogenes. Appl Environ Microbiol. 2003 Jan;69(1):1-9. DOI: 10.1128/AEM.69.1.1-9.2003
5. Vazquez-Boland JA, Kuhn M, Berche, P, Chakraborty, T, Dominguez-Bernal G, Goebel W, et al. Listeria pathogenesis and molecular virulence determinants. Clin Microbiol Rev. 2001 July; 14(3):584-640. DOI: 10.1128/CMR.14.3.584-640.2001
6. Charpentier E, Courvalin P. Antibiotic resistance in Listeria spp. Antimicrob Agents Chemother. 1999 Sept;43(9):2103-8.
7. Altuntas EG, Kocan D, Cosansu E, Ayhan K, Juneja VK, Materon L. Antibiotic and bacteriocin sensitivity of Listeria monocytogenes strains isolated from different foods. JFNS. 2012 Mar;3(3):363-8. DOI: 10.4236/ fns.2012.33052
8. Lotfollahi L, Nowrouzi J, Irajian G, Masjedian F, Kazemi B, Eslamian L, et al. Prevalence and antimicrobial resistance profiles of Listeria monocytogenes in spontaneous abortions in humans. Afr J Microbiol Res. 2011 July;5(14):1990-3. DOI: 10.5897/AJMR11.498
9. Yücel N, Çitak S, Önder M. Prevalence and antibiotic resistance of Listeria species in meat products in Ankara, Turkey. Food Microbiol. 2005 Apr;22(2):241-5. DOI: 10.1016/j.fm.2004.03.007
10. SR EN ISO 11290-1, Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of Listeria monocytogenes - Part 1: Detection method. 2000.
11. SR EN ISO 11290-2, Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of Listeria monocytogenes - Part 2: Enumeration method. 2000.
12. SR EN ISO 11290-2/A1, Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of Listeria monocytogenes - Part 2: Enumeration method, Amendament 1: Modification of the enumeration medium. 2005.
13. Clinical and Laboratory Standards Institute (CLSI), Performance standards for antimicrobial susceptibility testing; sixteenth informational supplement, NCCLS document M100-S16, CLSI. 2006;26(3):44-55.
14. Carp-Cărare C, Vlad-Sabie A, Floriştean CV. Detection and serotyping of Listeria monocytogenes in some food products from North-East of Romania. Rev Romana Med Lab. 2013 Sep;21(3):285-92. DOI: 10.2478/rrlm-2013-0025
15. Gelbičova T, Karpiškova R. Outdoor environment as a source of Listeria monocytogenes in food chain. Czech J Food Sci. 2012;30(1):83-8.
16. Rahimi E, Momtaz H, Sharifzadeh A, Bezhadnia A, Ashtari MS, Esfahani SZ, et al. Prevalence and antimicrobial resistance of Listeria monocytogenes species isolated from traditional dairy products in Chahar Mahal and Bakhtiyari, Iran. Bulg J Vet Med. 2012 June; 15(2):115-22.
17. De Nes F, Riboldi GP, Frazzon APG, D’azevedo PA, Frazzon J. Antimicrobial resistance and investigation of the molecular epidemiology of Listeria monocytogenes in dairy products. Rev Soc Bras Med Trop. 2010 July/Aug;43(4):382-5. DOI: 10.1590/S0037-86822010000400009
18. Wang F, Chrn M, Li C, Yan M, Hsieh Y. Prevalence and antibiotic resistance of Listeria species in food products in Taipei, Taiwan. Afr J Microbiol Res. 2012 June;6(22):4702-6.
19. Ennaji H, Timinouni M, Ennaji M, Hassar M, Cohen N. Characterization and antibiotic susceptibility of Listeria monocytogenes isolated from poultry and red meat in Marocco. Infect Drug Resist. 2008 Sep;1:45-50.
20. Krawczyk-Balska A, Marchlewicz J, Dudek D, Wasiak K, Samluk A. Identification of a ferritin-like protein of Listeria monocytogenes as a mediator of β-lactam tolerance and innate resistance to cephalosporins. BMC Microbiol. 2012 Nov;12:278. DOI: 10.1186/1471-2180-12-278
21. Kovacevic J, Sagert J, Wozniak A, Gilmour MW, Allen KJ. Antimicrobial resistance and co-selection phenomenon in Listeria spp. recovered from food and food production environments. Food Microbiol. 2013 Jun;34(2):319-27. DOI: 10.1016/j.fm.2013.01.002
22. Bertsch D, Muelli M, Weller M, Uruty A, Lacroix C, Meile L. Antimicrobial susceptibility and antibiotic resistance gene transfer analysis of foodborne, clinical, and environmental Listeria spp. isolates including Listeria monocytogenes. Microbiologyopen. 2014 Jan;[Epub ahead of print].
23. Korsak D, Borek A, Daniluk S, Grabowska A, Pappelbaum K. Antimicrobial susceptibilities of Listeria monocytogenes strains isolated from food and food processing environment in Poland. Int J Food Microbiol. 2012 Sep;158(3):203-8. DOI: 10.1016/j.ijfoodmicro. 2012.07.016