Evaluation of the gametotoxic effect of nandrolone decanoate on the gametogenic function of male rabbit

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Abstract

The research was conducted on a number of 24 sexually mature male rabbits of common breed, divided into two numerically equal groups. The subjects from the experimental group were given 20 mg/kg nandrolone decanoate, intramuscularly, twice a week for four consecutive weeks. The control group did not receive any treatment. At the end of the experiment the testes were harvested through bilateral orchiectomy and processed for carrying out histopathological investigations. Testicular fragments were fixated in Stieve’s mixture for 24 hours, dehydrated with alcohol, clarified with butyl alcohol and included in paraffin. Serial sections of 5 μ thickness were stained by Masson's trichrome method modified by Goldner. The testes taken from the control animals showed no detectable changes in optical microscopy. In the case of the animals belonging to experimental lots, there were alterations revealed evidenced by vacuolar dystrophy in the spermatocytes and spermatids, apoptosis, the edema of sperm head, spermatids syncytialization and cell necrosis followed by the disintegration of the seminal cell line with the formation of cellular debris in the seminiferous tubules lumen. These changes have affected part of the seminal cell line from the adluminal compartment (primary and secondary spermatocytes, spermatids and spermatozoids), but not those from the basal compartment (type A1 and B spermatogonia), which proved to be resistant in the conditions of 30 days exposure, at a dose of 20 mg/kg. In this context spermatogenesis is affected only for the period of drug action, but the risk of nandrolone decanoate transmission to humans through animal origin food consumption remains, with negative consequences on the spermatogenesis.

1. Rajesh KN. Endocrine Disruptors: Effects on Male and Female Reproductive Systems. Hardcover Jan 21. 2009; 149-153;

2. Karbalay-Doust S, Noorafshan A, Ardekani FM, Mirkhani H, Baker G. The reversibility of sperm quality after discontinuing nandrolone decanoate in adult male rats. Asian Journal of Andrology. 2007;9(2):235-9.

3. Pinelda MH, Dooley MP. McDonald’s Fifth Edition, Veterinary endocrinology and reproduction. Blackwel Publishing. 2003, 432-436

4. Creasy D, Cartwright J, Moreland S, Willoughby C, Collier M, Odum J. Endocrine disruption: Aguidance document for histologic evaluation of endocrine and reproductive tests. Syngenta, 2008.2-5

5. Bjorndahl L, Mortimer D, Barratt CLR, Castilla JA, Menkveld R, Kvist U, Alvarez JG, Haugen TB. A practical guide to basic laboratory andrology. Cambridge University Press. 2010;114-116

6. Anderson D. Brinkwoth MH. Male-mediated Developmental Toxicity, 2007, RSC Publishing; 173-178

7. Lubna HT, Noor HM, Iman NH, Iman MA, Salem RY, Maher IA. Nandrolone decanoat administration to male rats induces oxidative stress, seminiferous tubules abnormalities and sperm DNA fragmentation. 2010; 165-174

8. Aydilek N, Aksaka M, Karaklicik AZ. Effects of testosterone and vitamin E on the antioxidant system in rabbit testis. Andrologia. 2004 Sep, 277-281

9. Anderson D. Male-mediated Developmental Toxicity. Toxicol Appl Pharmacol. 2005 Sep 1;207 (2 Suppl):506-13

10. Creasy, D. M. Evaluation of testicular toxicology: a synopsis and discussion of the recommendations proposed by the Society of Toxicologic Pathology. Birth Defects Res B Dev Reprod Toxicol 68, 2003. 408-415.

11. Blanco-Rodriguez J, Martinez-Garcia C, Porras A. Correlation between DNA synthesis in the second, third and fourth generations of spermatogonia and the occurrence of apoptosis in both spermatogonia and spermatocyte. Reproduction. 2003; 126 (5):661-8

12. Feldman EC, Nelson RW. Canine and feline endocrinology and reproduction. Saunders. 2004, 987-1005

13. Pop AR, Groza I, Miclaus V, Simona Ciupe, Gabriela Mutiu, Borzan M. The influence of hexoestrol diacetate on gametogene function in male rabbit. Romanian Journal of Morphology and Embryology 2011, 52(1 Suppl): 413-417

Revista Romana de Medicina de Laborator

Romanian Journal of Laboratory Medicine

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