Research on antioxidant potential from vegetables is increasingly focused on their effects on human health. However, relatively little work has been done to investigate the antioxidant effect of crude extract and/or different fractions from Brassica rapa L. In the present study, the antioxidant potential of crude extract and its fractions from Brassica rapa L. fruit part was tested for glutathione peroxidase (GPx), superoxide dismutase (SOD) enzymes and total antioxidant status (TAS) in blood samples. Our results reveal the fact that crude extract and each analyzed fraction (i.e. aqueous, ethyl acetate and chloroform) showed a concentration dependent effects on GPx, SOD and TAS in respect with saline solution (0.9% NaCl) used as negative control and vitamin C, as positive control. Therefore, GPx levels showed a highest value in crude extract and chloroform fraction (6981 U/L both at 10 mg/ml), SOD levels showed the same results in aqueous and ethyl acetate fractions (220 U/ml both at 10 mg/ml) and TAS in crude extract and all three fractions (i.e. aqueous, ethyl acetate and chloroform, 1.68 mmol/L at 10 mg/ml for all three fractions) in respect with saline solution (p<0.05). Furthermore, vitamin C showed the highest values on all three analyzed enzymes (8769 U/L for GPx, 223 U/ml for SOD and 1.8 mmol/L for TAS at 100 μg/ml). Our investigations have been proved to be promising in terms of future potential applications of crude extract and its fractions as components in a range of phytochemicals composition and/or different pharmaceutical usage, owing to their antioxidant potential.
1. Iqbal S, Bhanger MI, Anwar F. Antioxidant properties and components of bran extracts from selected wheat varieties commercially available in Pakistan. LWT Journal of Food Science and Technology 2007; 40, 361-7.
2. Siddhuraju P, Becker K. Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves. J. Agric. Food Chem. 2003; 51: 2144-55.
3. Ryu JP, Kim DC, In MJ, Chae HJ, Lee SD. Antioxidant potential of ethanol extract of Brassica rapa L. J. Med. Plants Res. 2012; 6(9):1581-4., DOI: 10.5897/JMPR11.1068
4. Podsedek A. Natural antioxidants and antioxidant capacity of Brassica vegetables: A review. LWT Food Science and Technology, 2004; 40(1): 1-11.
5. Silva BM, Andrade PB, Valentao P, Ferreres F, Seabra R M, Ferreira MA. Quince (Cydonia oblonga Miller) fruit (pulp, peel and seed) and jam: Antioxidant activity. J. Agric. Food Chem. 2004; 52, 4705-12.
6. Vaughan JG, Geissler CA. The New Oxford Book of Food Plants. New York: Oxford University Press. 1997; 196.
8. Fernandes F, Valentao P, Sousa C, Pereira JA, Seabra RM, Andrade PB. Chemical and antioxidative assessment of dietary turnip (Brassica rapa var. rapa L.). Food Chemistry 2007; 105: 1003-10.
9. Vallejo F, Tomas-Barberan FA, Ferreres F. Characterization of flavonols in broccoli (Brassica oleracea L. var. italica) by liquid chromatography-UV diodearray detection- lectrospray ionisation mass spectrometry. Journal of Chromatography A 2004; 1054: 181-93.
10. Romani A, Vignolini P, Isolani L, Ieri F, Heimler D. (2006). HPLC-DAD/MS characterization of flavonoids and hydroxycinnamic derivatives in turnip tops (Brassicarapa L. Subsp. sylvestris L.). J. Agric. Food Chem. 2006; 54: 1342-6.
11. Sousa C, Valentao P, Rangel J, Lopes G, Pereira JA, Ferreres F, et al. Influence of two fertilization regimens on the amounts of organic acids and phenolic compounds of tronchuda cabbage (Brassica oleraceae L. var. costata DC). J. Agric. Food Chem. 2005; 53: 9128-32.
12. Ferreres F, Sousa C, Vrchovska V, Valentao P, Pereira JA, Seabra RM, et al. Chemical composition and antioxidant activity of tronchuda cabbage internal leaves. European Food Research & Technology 2006; 222: 88-98.
13. Heimler D, Vignolini P, Dini MG, Vincieri FF, Ro- mani A. Antiradical activity and polyphenol composition of local Brassicaceae edible varieties. Food Chemistry 2006; 99: 464-469.
14. Liang YS, Kim HK, Lefeber AWM, Erkelens C, Choi YH, Verpoorte R. Identification of phenylpropanoids in methyl jasmonate treated Brassica rapa leaves using two-dimensional nuclear magnetic resonance spectroscopy. Journal of Chromatography A 2006; 1112: 148-55.
15. Vrchovska V, Sousa C, Valentao P, Ferreres F, Pereira JA, Seabra RM et al. (2006). Antioxidative properties of tronchuda cabbage (Brassica oleraceae L. var. costata DC) external leaves against DPPH, superoxide radical, hydroxyl radical and hypochlorous acid. Food Chemistry 2006; 98: 416-25.
16. Johnston CS, Taylor CA, Hampl JS. More Americans are eating ‚5 A Day’ but intakes of dark green and cruciferous vegetables remain low. J. Nutr. 2000; 130:3063-7.
17. Cao G, Booth SL, Sadowski JA, Prior RL. Increases in human plasma antioxidant capacity after consumption of controlled diets high in fruit and vegetables. Am. J. Clin. Nutr. 1998; 68:1081-7.
18. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J. Lab. Clin. Med. 1967; 70: 158.
19. Oyanagui Y. Reevaluation of assay methods and establishment of kit for superoxide dismutase activity. Anal. Biochem. 1984; 142(2): 290-6.
20. Mitrevky A et al. Total antioxidant status and superoxide dismutase after cerebrovascular accident. XVI international Congress of clinical chemistry, London. Poster A106, 1996.
21. Cohen J, Kristal R, Stanford J. Fruit and vegetable intakes and prostate cancer risk. J. Natl. Cancer Inst. 2000; 92: 61-8.
22. Onyilagha J, Bala A, Hallett R, Gruber M, Soroka J, Westcott N. Leaf flavonoids of the cruciferous species, Camelina satiVa, Grambe spp., Thlaspi arVense and several other genera of the family Brassicaceae. Biochem. Syst. Ecol. 2003; 31: 1309-22.
23. Pedroche J, Yust MM, Lqari H, Giron-Calle J, Alaiz M, Vioque J, Millán F. Brassica carinata protein isolates: chemical composition, protein characterization and improvement of functional properties by protein analysis. Food Chemistry 2004; 88: 337-46.
24. Jang IC, Park JH, Park E, Park HR, Lee FJ. Antioxidative and antigenotoxic activity of extracts from Cosmos (Cosmos bipinnatus) flowers, Plant Foods Hum. Nutr. 2008; 63: 205-10.
25. Wada L, Ou B. Antioxidant activity and phenolic content of Oregon cranberries, J. Agric. Food Chem. 2002; 50: 3495-500.
26. Kähkönen MP, Hopia AI, Vuorela HJ, Rauha JP, Pihlaja K, Kujala TS, Heinonen M. Antioxidant activity of plant extracts containing phenolic compounds. J. Agric. Food Chem. 1999; 47: 3954-62.
27. Chu YH, Chang CL, Hsu H.F. Flavonoid content of several vegetables and their antioxidant activity. J. Agric. Food Chem. 2000; 80, 561-6.
28. Tang S, Kerry JP, Sheehan D, Buckley DE, Morrisey PA. Anti-oxidant activity of added tea catechins on lipid oxidation of raw mince red meat, poultry and ®sh muscle. International Journal of Food Science & Technology 2001; 36: 1-8.
29. Ou B, Huang D, Hampsch-Woodill M, Flanagan JA, Deemer EK. Analysis of antioxidant activities of common vegetables employing oxigen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) assays: A comparative study. J. Agric. Food Chem. 2002; 50: 3122-8.
30. Nain P, Saini V, Sharma S, Nain J. Antidiabetic and antioxidant potential of Emblica officinalis Gaertn. leaves extract in streptozotocin-induced type-2 diabetes mellitus (T2DM) rats. J. Ethnopharmacol. 2012; 142(1): 65-71.
31. Shah K, Verma RJ. Protection against butyl p-hydroxybenzoic acid induced oxidative stress by Ocimum sanctum extract in mice liver. Acta Pol. Pharm. 2012;69(5): 865-70.
32. Abrahim NN, Kanthimathi MS, Abdul-Aziz A. Piper betle shows antioxidant activities, inhibits MCF-7 cell proliferation and increases activities of catalase and superoxide dismutase. BMC Complementary and Alternative Medicine 2012;12: 220.
33. Mahdy K, Shaker O, Wafay H, Nassar Y, Hassan H, Hussein A. Effect of some medicinal plant extracts on the oxidative stress status in Alzheimer's disease induced in rats. Eur. Rev. Med. Pharmacol. Sci. 2012;16(3): 31-42.
34. Saeed N, Khan MR, Shabbir M. Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Complementary and Alternative Medicine 2012;12: 221.