Use of DNA markers for cereal line uniformity assessment
Prior to the registration of a new variety, it is required to undergo Distinctness, Uniformity and Stability (DUS) testing. Preparing a newly developed variety to meet the requirements of DUS testing is a lengthy process, particularly regarding aspects of uniformity and stability. Field testing of a large number of lines is time and resource intensive. In addition, the expression of certain traits may be influenced by environmental conditions. The use of DNA markers may allow rapid assessment of the level of genetic diversity within a particular line or variety, and to remove individuals that are genetically differentiated, thus accelerating the homogenisation of a newly developed variety. In this study, we utilised AFLP and the iPBS marker techniques to assess genetic variation within advanced breeding lines of several cereal species (triticale, wheat, barley). The combined use of molecular and morphological selection over three years of analysis and selection resulted in the reduction of genetic diversity within breeding lines.
Burton, W.A., Ripley, V.L., Potts, D.A., Salisbury P.A. (2004). Assessment of genetic diversity in selected breeding lines and cultivars of canola quality Brassica juncea and their implications for canola breeding. Euphytica, 136, 181-192.
DellaVecchia, P.T., Da Silva, C.A.R. & Terenciano-Sobrinho, P. (1998). Use of molecular marker techniques in seed testing by Brazilian seed companies. Sci. Agric. (Piracicaba, Braz.), 55, 79-82.
Dillmann, C., Bar-Hen, A., Guérin, D., Charcosset, A., Murigneux (1997). Comparison of RFLP and morphological distances between maize Zea mays L. inbred lines. Consequences for germplasm protection purposes. Theor. Appl. Genet., 95, 92-102.
Doyle, J.J., Doyle, J.L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull., 19, 11-15.
Kalendar, R., Antonius, K., Smżkal, P., Schulman, A.H. 2010. iPBS: A universal method for DNA fingerprinting and retrotransposon isolation. Theor. Appl. Genet., 121, 1419-1430.
Lombard, V., Tireau, B., Blouet, F., Zhang, D.,Baril, C.P. (2002). Usefulness of AFLP markers to estimate varietal homogeneity of rapeseed inbred line varieties in the context of plant registration and protection. Euphytica, 125, 121-127.
Powell, W., Morgante, M., Andre, C., Hanafey, M., Vogel, J., Tingey, S. (1996). The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol. Breed., 2, 225-238.
Roldán-Ruiz, I., Dendauw, J., Van Bockstaele, E., Depicker, A., De Loose, M. (2000). AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.). Mol. Breed., 6, 125-134.
Sabot, F., Schulman, A.H. (2006). Parasitism and the retrotransposon life cycle in plants: A hitchhiker's guide to the genome. Heredity, 97, 381-388.
Singh, R.K., Sharma, R.K., Singh, A.K., Singh, V.P., Singh, N.K., Tiwari, S.P., Mohapatra, T. (2004). Suitability of mapped sequence tagged microsatellite site markers for establishing distinctness, uniformity and stability in aromatic rice. Euphytica, 135, 135-143.
Vos, P., Hogers, R., Bleeker, M., Reijans, M., van de Lee, T., Hornes, M., Friters, A., Pot, J., Paleman, J., Kuiper, M., Zabeau, M. (1995). AFLP: A new technique for DNAfingerprinting. Nucleic Acids Res., 23, 4407-4414. doi:10.1093/nar/23.21.4407.