A comparison of the efficacy and pharmacokinetics of ivermectin after spring and autumn treatments against Cyathostominae in horses

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The aim of the present study was to determine the efficacy of ivermectin against Cyathostominae infections and to describe the drug’s pharmacokinetic parameters during two seasonal deworming treatments in horses. The study was performed on warm-blooded mares aged 3-12 years weighing 450-550 kg. A single bolus of an oral paste formulation of ivermectin was administered at a dose of 0.2 mg/kg BW in spring and autumn. Fecal samples were tested before treatment and 1, 2, 3, 4, 6, 10, 20, 30, 40, 50, 60, 75 days after treatment. Ivermectin concentrations in blood samples collected before treatment, 0.5, 1, 2, 3, 4, 6, 12, 24, 36 and 48 hours after treatment, and 3, 4, 6, 8, 10, 15, 20, 25, 30, 40, 50, 60 and 75 days after drug administration were determined by high pressure liquid chromatography. Drug absorption was significantly (p<0.05) slower (tmax: 21.89±11.43 h) in autumn than in spring (tmax: 9.78±8.97 h). Maximum concentrations (Cmax) of ivermectin in the blood plasma of individual horses (8.40-43.08 ng/ml) were observed 2-24 h after drug administration during the spring treatment and 2-36 h (6.43-24.86 ng/ml) after administration during the autumn treatment. Significantly higher (p<0.05) ivermectin concentrations were found during the first 4 hours after administration in spring in comparison with those determined after the autumn treatment.

The administration of the recommended dose of ivermectin resulted in 100% elimination of parasitic eggs from feces in spring and autumn treatment.

Abbott JB, Mellor DJ, Love S (2007) Assessment of serum protein electrophoresis for monitoring therapy of naturally acquired equine cyathostomin infections. Vet Parasitol 147: 110-117.

Bello TR (1996) Alternative antiparasitic treatment of horses with pyrantel pamoate suspension and ivermectin oral solution compared with horses treated only with ivermectin. J Equine Vet Sci 16: 18-21.

Chapman MR, Kearney MT, Klei TR (2003) Equine cyathostome populations: accuracy of species composition estimations. Vet Parasitol 116: 15-21.

Demeulenaere D, Vercruysse J, Dorny P, Claerebout E (1997) Comparative studies of ivermectin and moxidectin in the control of naturally acquired cyathostome infections in horses. Vet Rec 141: 383-386.

Drudge JH, Lyons ET (1989) Internal parasites of equids with emphasis on treatment and control. (Revision) Monopgraph, Hoechest-Roussel Agri-Vet, Somerville, NJ, p 26.

Dupuy J, Yin H, Luo JX, Yang DY, Guan GQ, Ma ML, Sutra JF, Lespine A, Boulard C, Alvinerie M (2003) Pharmacokinetics of ivermectin in the yak (Bos grunniens). Vet Parasitol 117: 153-157.

Eraslan G, Kanbur M, Liman BC, Cam Y, Karabacak M, Altinordulu S (2010) Comparative pharmacokinetics of some injectable preparations containing ivermectin in dogs. Food Chem Toxicol 48: 2181-2185.

Gawor J (2002) Prevalence of intestinal parasites in riding horses. Med Weter 58: 148-150.

Gawor J, Kita J (2006) Practical observations on antiparasitic treatment in horses. Życie Wet 81: 753-756.

Gokbulut C, Cirak VY, Senlik B, Aksit D, Durmaz M, McKellar QA (2010) Comparative plasma disposition, bioavailability and efficacy of ivermectin following oral and pour-on administrations in horses. Vet Parasitol 170: 120-126.

Gokbulut C, Karademir U, Boyacioglu M, McKellar QA (2006) Comparative plasma dispositions of ivermectin and doramectin following subcutaneous and oral administration in dogs. Vet Parasitol 135: 347-354.

Gokbulut C, Nolan AM, McKellar QA (2001) Plasma pharmacokinetics and faecal excretion of ivermectin, doramectin and moxidectin following oral administration in horses. Equine Vet J 33: 494-498.

Kornaś S, Skalska M, Nowosad B, Gawor J (2007) The communities of cyathostomes (Cyathostominae) in one-year-old and two-year-old Pure Blood Arabian mares. Wiad Parazytol 53: 325-329.

Kowalski P, Bieniecki M, Olędzka I, Lamparczyk H (2004) Validated capillary electrophoretic method for the analysis of ivermectin in plasma after intragastric administration in pigs and horses. Biomed Chromatogr 18: 302-310.

Lanusse CE, Lifschitz AL, Imperiale FA (2009) Macrocyclic lactones: endectocide compounds. In: Riviere JE, Papich MG (eds.) Veterinary pharmacology and therapeutics 9th ed. Blackwell Publishing, Ames, Iowa, USA, pp. 1119-1145.

Lichtenfels JR, Kharchenko VA, Dvojnos G M (2008) Illustrated identification keys to strongylid parasites (Strongylidae Nematoda) of horses, zebras and asses (Equidae). Vet Parasitol 156: 4-161.

Love S, Murphy D, Mellor D (1999) Pathogenicity of cyathostome infection. Vet Parasitol 85: 113-122.

Lyons ET, Tolliver SC, Drudge JH (1999) Historical perspective of cyathostomes: prevalence, treatment and control programs. Vet Parasitol 85: 97-112.

Matthews JB, Hodgkinson JE, Dowdall SM, Proudman CJ (2004) Recent developments in research into the Cyathostominae and Anoplocephala perfoliata. Vet Res 35: 371-381.

Osterman Lind E, Eysker M, Nilsson O, Uggla A, Hoglund J (2003) Expulsion of small strongyle nematodes (cyathostomin spp) following deworming of horses on a stud farm in Sweden. Vet Parasitol 115: 289-299.

Perez R, Cabezas I, Garcta M, Rubilar L, Sutra JF, Galtier P, Alvinerie M (1999) Comparison of the pharmacokinetics of moxidectin (Equest) and ivermectin (Eqvalan) in horses. J Vet Pharmacol Ther 22: 174-180.

Perez R, Cabezas I, Godoy C, Rubilar L, Muñoz L, Arboix M, Castells G, Alvinerie M (2002) Pharmacokinetics of doramectin and ivermectin after oral administration in horses. Vet J 163: 161-167.

Perez R, Godoy C, Palma C, Cabezas I, Muńoz L, Rubilar L, Arboix M, Alvinerie M (2003) Plasma profiles of ivermectin in horses following oral or intramuscular administration. J Vet Med A Physiol Pathol Clin Med 50: 297-302.

Pitterna T, Cassayre J, Huter OF, Jung PM, Maienfisch P, Kessabi FM, Quaranta L, Tobler H (2009) New ventures in the chemistry of avermectins. Bioorg Med Chem 17: 4085-4095.

Reinemeyer CR, Clymer BC (2002) Comparative efficiency of moxidectin gel or ivermectin paste for Cyathostome control in young horses. J Equine Vet Sci 22: 33-36.

Reinemeyer CR, Henton JE (1987) Observations on equine strongyle control in southern temperate USA. Equine Vet J 19: 505-508.

Steinbach T, Bauer C, Sasse H, Baumgartner W, Rey-Moreno C, Hermosilla C, Damriyasa IM, Zahner H (2006) Small strongyle infection: consequences of larvicidal treatment of horses with fenbendazole and moxidectin. Vet Parasitol 139: 115-131.

Traversa D (2010) Anthelmintic resistance in horse cyathostomins in Europe: current status and future perspectives. Sci Pratasitol 11: 1-6.

Von Samson-Himmelstjerna G, Fritzen B, Demeler J, Schürmann S, Rohn K, Schnieder T, Epe C (2007) Cases of reduced cyathostomin egg-reappearance period and failure of Parascaris equorum egg count reduction following ivermectin treatment as well as survey on pyrantel efficacy on German horse farms. Vet Parasitol 144: 74-80.

Whitlock H (1948) Some modifications of the McMaster helminth egg-counting technique and apparatus. J Counc Sci Res 21: 177-180.

Ziomko I, Cencek T (1999) Inwazje pasożytnicze zwierząt gospodarskich – wybrane metody diagnostyczne. 1st ed., Drukarnia Piotra Włodarskiego, Warszawa.

Polish Journal of Veterinary Sciences

The Journal of Committee of Veterinary Sciences of Polish Academy of Sciences and University of Warmia and Mazury in Olsztyn

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