The efficiency of somatic cell cloning (somatic cell nuclear transfer; SCNT) as well as in vitro fertilization/in vitro embryo production (IVF/IVP) in mammals stay at relatively same level for over a decade. Despite plenty of different approaches none satisfactory break-through took place. In this article, we briefly summarize the implementation of mesenchymal stem cells (MSCs) for experimental embryology. The advantages of using MSCs as nuclear donors in somatic cell cloning and in vitro embryo culture are described. The description of results obtained with these cells in mammalian embryo genomic engineering is presented.
Baer PC, Bereiter-Hahn J, Missler C, Brzoska M, Schubert R, Gauer S, Geiger H (2009) Conditioned medium from renal tubular epithelial cells initiates differentiation of human mesenchymal stem cells. Cell Prolif 42: 29-37.
Baer PC, Geiger H (2010) Mesenchymal stem cell interactions with growth factors on kidney repair. Curr Opin Nephrol Hypertens 19: 1-6.
Banfi A, Muraglia A, Dozin B, Mastrogiacomo M, Cancedda R, Quarto R (2000) Proliferation kinetics and differentiation potential of ex vivo expanded human bone marrow stromal cells: Implications for their use in cell therapy. Exp Hematol 28: 707-715.
Bruder SP, Kurth AA, Shea M, Hayes WC, Jaiswal N, Kadiyala S (1998) Bone regeneration by implantation of purified, culture-expanded human mesenchymal stem cells. J Orthop Res 16: 155-162.
Caplan AI (2007) Adult mesenchymal stem cells for tissue engineering versus regenerative medicine. J Cell Physiol 213: 341-347.
Clarke E, McCann SR (1989) Age dependent in vitro stromal growth. Bone Marrow Transplant 4: 596-597.
Colleoni S, Donofrio G, Lagutina I, Duchi R, Galli C, Lazzari G (2005) Establishment, differentiation, electroporation, viral transduction, and nuclear transfer of bovine and porcine mesenchymal stem cells. Cloning Stem Cells 7: 154-166.
Costa-Borges N, Santaló J, Ibhnez E (2010) Comparison between the effects of valproic acid and trichostatin A on the in vitro development, blastocyst quality, and full-term development of mouse somatic cell nuclear transfer embryos. Cell Reprogram 12: 437-446.
Crisan M, Yap S, Casteilla L, Chen CW, Corselli M, Park TS, Andriolo G, Sun B, Zheng B, Zhang L, Norotte C, Teng PN, Traas J, Schugar R, Deasy BM, Badylak S, Buhring HJ, Giacobino JP, Lazzari L, Huard J, Peault B (2008) A perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell 3: 301-313.
Dai X, Hao J, Hou XJ, Hai T, Fan Y, Yu Y, Jouneau A, Wang L, Zhou Q (2010) Somatic nucleus reprogramming is significantly improved by m-carboxycinnamic acid bishydroxamide, a histone deacetylase inhibitor. J Biol Chem 285: 31002-31010.
Das ZC, Gupta MK, Uhm SJ, Lee HT (2010) Increasing histone acetylation of cloned embryos, but not donor cells, by sodium butyrate improves their in vitro development in pigs. Cell Reprogram 12: 95-104.
Deng J, Petersen BE, Steindler DA, Jorgensen ML, Laywell ED (2006) Mesenchymal stem cells spontaneously express neural proteins in culture and are neurogenic after transplantation. Stem Cells 24: 1054-1064.
D’Ippolito G, Diabira S, Howard GA, Menei P, Roos BA, Schiller PC (2004) Marrow-isolated adult multilineage inducible (MIAMI) cells, a unique population of postnatal young and old human cells with extensive expansion and differentiation potential. J Cell Sci 117: 2971-2981.
Faast R, Harrison SJ, Beebe LF, McIlfatrick SM, Ashman RJ, Nottle MB (2006) Use of adult mesenchymal stem cells isolated from bone marrow and blood for somatic cell nuclear transfer in pigs. Cloning Stem Cells 8: 166-173.
Ho AD, Wagner W, Franke W (2008) Heterogeneity of mesenchymal stromal cell preparations. Cytotherapy 10: 320-330.
Hofstetter CP, Schwarz EJ, Hess D, Widenfalk J, El Manira A, Prockop DJ, Olson L (2002) Marrow stromal cells form guiding strands in the injured spinal cord and promote recovery. Proc Natl Acad Sci USA 99: 2199-2204.
Horn P, Bork S, Diehlmann A, Walenda T, Eckstein V, Ho AD, Wagner W (2008) Isolation of human mesenchymal stromal cells is more efficient by red blood cell lysis. Cytotherapy 10: 676-685.
Horn P, Bokermann G, Cholewa D, Bork S, Walenda T, Koch C, Drescher W, Hutschenreuther G, Zenke M, Ho AD, Wagner W (2010) Impact of individual platelet lysates on isolation and growth of human mesenchymal stromal cells. Cytotherapy 12: 888-898.
Horwitz EM, Le Blanc K, Dominici M, Mueller I, Slaper-Cortenbach I, Marini FC, Deans RJ, Krause DS, Keating A (2005) Clarification of the nomenclature for MSC: The International Society for Cellular Therapy position statement. Cytotherapy 7: 393-395.
Iager AE, Ragina NP, Ross PJ, Beyhan Z, Cunniff K, Rodriguez RM, Cibelli JB (2008) Trichostatin A improves histone acetylation in bovine somatic cell nuclear transfer early embryos. Cloning Stem Cells 10: 371-379.
Inoue K, Noda S, Ogonuki N, Miki H, Inoue S, Katayama K, Mekada K, Miyoshi H, Ogura A (2007) Differential developmental ability of embryos cloned from tissue-specific stem cells. Stem Cells 25: 1279-1285.
Jeon BG, Coppola G, Perrault SD, Rho GJ, Betts DH, King WA (2008) S-adenosylhomocysteine treatment of adult female fibroblasts alters X-chromosome inactivation and improves in vitro embryo development after somatic cell nuclear transfer. Reproduction 135: 815-828.
Jiang Y, Jahagirdar BN, Reinhardt RL, Schwartz RE, Keene CD, Ortiz- Gonzalez XR, Reyes M, Lenvik T, Lund T, Blackstad M, Du J, Aldrich S, Lisberg A, Low WC, Largaespada DA, Verfaillie CM (2002) Pluripotency of mesenchymal stem cells derived from adult marrow. Nature 418: 41-49.
Jin HF, Kumar BM, Kim JG, Song HJ, Jeong YJ, Cho SK, Balasubramanian S, Choe SY, Rho GJ (2007) Enhanced development of porcine embryos cloned from bone marrow mesenchymal stem cells. Int J Dev Biol 51: 85-90.
Jones E, McGonagle D (2008) Human bone marrow mesenchymal stem cells in vivo. Rheumatology (Oxford) 47: 126-131.
Kato Y, Imabayashi H, Mori T, Tani T, Taniguchi M, Higashi M, Matsumoto M, Umezawa A, Tsunoda Y (2004) Nuclear transfer of adult bone marrow mesenchymal stem cells: developmental totipotency of tissue- specific stem cells from an adult mammal. Biol Reprod 70: 415-418.
Kim YJ, Ahn KS, Kim M, Shim H (2011) Comparison of potency between histone deacetylase inhibitors trichostatin A and valproic acid on enhancing in vitro development of porcine somatic cell nuclear transfer embryos. In Vitro Cell Dev Biol Anim 47: 283-289.
Kumar BM, Jin HF, Kim JG, Ock SA, Hong Y, Balasubramanian S, Choe SY, Rho GJ (2007) Differential gene expression patterns in porcine nuclear transfer embryos reconstructed with fetal fibroblasts and mesenchymal stem cells. Dev Dyn 236: 435-446.
Lee HS, Yu XF, Bang JI, Cho SJ, Deb GK, Kim BW, Kong IK (2010) Enhanced histone acetylation in somatic cells induced by a histone deacetylase inhibitor improved inter- generic cloned leopard cat blastocysts. Theriogenology 74: 1439-1449.
Lee SL, Kang EJ, Maeng GH, Kim MJ, Park JK, Kim TS, Hyun SH, Lee ES, Rho GJ (2010) Developmental ability of miniature pig embryos cloned with mesenchymal stem cells. J Reprod Dev 56: 256-262.
Martinez C, Hofmann TJ, Marino R, Dominici M, Horwitz EM (2007) Human bone marrow mesenchymal stromal cells express the neural ganglioside GD2: a novel surface marker for the identification of MSCs. Blood 109: 4245-4248.
Martinez-Diaz MA, Che L, Albornoz M, Seneda MM, Collis D, Coutinho AR, El-Beirouthi N, Laurin D, Zhao X, Bordignon V (2010) Pre- and postimplantation development of swine-cloned embryos derived from fibroblasts and bone marrow cells after inhibition of histone deacetylases. Cell Reprogram 12: 85-94.
Minguell JJ, Martı´nez J (1983) Growth pattern and function of bone marrow fibroblasts from normal and acute lymphoblastic leukemia patients. Exp Hematol 11: 522-526.
Moscoso I, Centeno A, López E, Rodriguez-Barbosa JI, Santamarina I, Filgueira P, Shnchez MJ, Domı´nguez-Perles R, Pen˜uelas-Rivas G, Domenech N (2005) Differentiation “in vitro” of primary and immortalized porcine mesenchymal stem cells into cardiomyocytes for cell transplantation. Transplant Proc 37: 481-482.
Oh SH, Witek RP, Bae SH, Zheng D, Jung Y, Piscaglia AC, Petersen BE (2007) Bone marrow-derived hepatic oval cells differentiate into hepatocytes in 2-acetylaminofluorene/ partial hepatectomy- induced liver regeneration. Gastroenterology 132: 1077-1087.
Oh SH, Witek RP, Bae SH, Darwiche H, Jung Y, Pi L, Brown A, Petersen BE (2009) Detection of transketolase in bone marrow-derived insulin-producing cells: benfotiamine enhances insulin synthesis and glucose metabolism. Stem Cells Dev 18: 37-46.
Phinney DG (2007) Biochemical heterogeneity of mesenchymal stem cell populations: clues to their therapeutic efficacy. Cell Cycle 6: 2884-2889.
Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284: 143-147.
Potapova IA, Gaudette GR, Brink PR, Robinson RB, Rosen MR, Cohen IS, Doronin SV (2007) Mesenchymal stem cells support migration, extracellular matrix invasion, proliferation, and survival of endothelial cells in vitro. Stem Cells 25: 1761-1768.
Ratajczak MZ, Kucia M, Majka M, Reca R, Ratajczak J (2004) Heterogeneous populations of bone marrow stem cells - are we spotting on the same cells from the different angles? Folia Histochem Cytobiol 42: 139-146.
Ratajczak MZ, Zuba-Surma EK, Wysoczynski M, Ratajczak J, Kucia M (2008) Very small embryonic-like stem cells: characterization, developmental origin, and biological significance. Exp Hematol 36: 742-751.
Reyes M, Lund T, Lenvik T, Aguiar D, Koodie L, Verfaillie CM (2001) Purification and ex vivo expansion of postnatal human marrow mesodermal progenitor cells. Blood 98: 2615-2625.
Sacchetti B, Funari A, Michienzi S, Di Cesare S, Piersanti S, Saggio I, Tagliafico E, Ferrari S, Robey PG, Riminucci M, Bianco P (2007) Self-renewing osteoprogenitors in bone marrow sinusoids can organize a hematopoietic microenvironment. Cell 131: 324-336.
Samiec M, Skrzyszowska M (2010a) Preimplantation developmental capability of cloned pig embryos derived from different types of nuclear donor somatic cells. Ann Anim Sci 10: 385-398.
Samiec M, Skrzyszowska M (2010b) The use of different methods of oocyte activation for generation of porcine fibroblast cell nuclear-transferred embryos. Ann Anim Sci 10: 399-411.
Samiec M, Skrzyszowska M (2012) Roscovitine is a novel agent that can be used for the activation of porcine oocytes reconstructed with adult cutaneous and fetal fibroblast cell nuclei. Theriogenology 78: 1855-1867.
Schallmoser K, Rohde E, Reinisch A, Bartmann C, Thaler D, Drexler C, Obenauf AC, Lanzer G, Linkesch W, Strunk D (2008) Rapid large-scale expansion of functional mesenchymal stem cells from unmanipulated bone marrow without animal serum. Tissue Eng Part C Methods 14: 185-196.
Scopes J, Ismail M, Marks KJ, Rutherford TR, Draycott GS, Pocock C, Gordon-Smith EC, Gibson FM (2001) Correction of stromal cell defect after bone marrow transplantation in aplastic anaemia. Br J Haematol 115: 642-652.
Shibata KR, Aoyama T, Shima Y, Fukiage K, Otsuka S, Furu M, Kohno Y, Ito K, Fujibayashi S, Neo M, Nakayama T, Nakamura T, Toguchida J (2007) Expression of the p16INK4A gene is associated closely with senescence of human mesenchymal stem cells and is potentially silenced by DNA methylation during in vitro expansion. Stem Cells 25: 2371-2382.
Skrzyszowska M, Samiec M, Słomski R, Lipiński D, Mały E (2008) Development of porcine transgenic nuclear-transferred embryos derived from fibroblast cells transfected by the novel technique of nucleofection or standard lipofection. Theriogenology 70: 248-259.
Tondreau T, Dejeneffe M, Meuleman N, Stamatopoulos B, Delforge A, Martiat P, Bron D, Lagneaux L (2008) Gene expression pattern of functional neuronal cells derived from human bone marrow mesenchymal stromal cells. BMC Genomics 9: 166.
Tse WT, Pendleton JD, Beyer WM, Egalka MC, Guinan EC (2003) Suppression of allogeneic T-cell proliferation by human marrow stromal cells: implications in transplantation. Transplantation 75: 389-397.
Vacanti V, Kong E, Suzuki G, Sato K, Canty JM, Lee T (2005) Phenotypic changes of adult porcine mesenchymal stem cells induced by prolonged passaging in culture. J Cell Physiol 205: 194-201.
Van Thuan N, Bui HT, Kim JH, Hikichi T, Wakayama S, Kishigami S, Mizutani E, Wakayama T (2009) The histone deacetylase inhibitor scriptaid enhances nascent mRNA production and rescues full-term development in cloned inbred mice. Reproduction 138: 309-317.
Wagner W, Ho AD (2007) Mesenchymal stem cell preparations - comparing apples and oranges. Stem Cell Rev 3: 239-248.
Wagner W, Bork S, Lepperdinger G, Joussen S, Ma N, Strunk D, Koch C (2010a) How to track cellular aging of mesenchymal stromal cells? Aging (Albany NY) 2: 224-230.
Wagner W, Ho AD, Zenke M (2010b) Different facets of aging in human mesenchymal stem cells. Tissue Eng Part B Rev 16: 445-453.
Yamanaka K, Sugimura S, Wakai T, Kawahara M, Sato E (2009) Acetylation level of histone H3 in early embryonic stages affects subsequent development of miniature pig somatic cell nuclear transfer embryos. J Reprod Dev 55: 638-644.
Zhao J, Hao Y, Ross JW, Spate LD, Walters EM, Samuel MS, Rieke A, Murphy CN, Prather RS (2010) Histone deacetylase inhibitors improve in vitro and in vivo developmental competence of somatic cell nuclear transfer porcine embryos. Cell Reprogram 12: 75-83