Occurrence of virulence genes among Campylobacter jejuni and Campylobacter coli isolates from domestic animals and children

Open access

Occurrence of virulence genes among Campylobacter jejuni and Campylobacter coli isolates from domestic animals and children

The presence of the flaA, cadF, cdtB and iam genes of Campylobacter spp. was determined with the PCR method. The materials to investigate were 56 C. jejuni and 23 C. coli strains isolated from clinical samples (children and domestic animals).

It was found that all of the Campylobacter spp. isolates from children with diarrhoea and domestic animals had cadF gene, responsible for adherence.

The flaA gene was present in all Campylobacter spp. isolates derived from children and cats. Occurrence of flaA gene was confirmed in 100% of C. jejuni strains obtained from dogs.

The high prevalence of the cdtB gene associated with toxin production was observed in this study (100%-Campylobacter spp. isolates obtained from dogs and cats, 97.9%-Campylobacter spp. isolates from children).

The isolates showed a wide variation for the presence of iam gene. The lowest prevalence (23.5%) was detected in Campylobacter spp. obtained from dogs. The highest rates of iam detection (91.6%) were revealed in C. coli isolates from children.

Al-Mahmeed A, Senok AC, Ismaeel AY, Bindayna KM, Tabbara KS, Botta GA (2006) Clinical relevance of virulence genes in Campylobacter jejuni isolates in Bahrain. J Med Microbiol 55: 839-843.

Bang DD, Scheutz F, Ahrens P, Pedersen K, Blom J, Madsen M (2001) Prevalence of cytolethal distending toxin (cdt) genes and CDT production in Campylobacter spp. isolated from Danish broilers. J Med Microbiol 50: 1087-1094.

Bereswill S, Kist M (2003) Recent developments in Campylobacter pathogenesis. Curr Opin Infect Dis 16: 487-491.

Carvalho AC, Ruiz-Palacios GM, Ramos-Cervantes P, Cervantes LE, Jiang X, Pickering LK (2001) Molecular characterization of invasive and noninvasive Campylobacter jejuni and Campylobacter coli isolates. J Clin Microbiol 39: 1353-1359.

Datta S, Niwa H, Itoh K (2003) Prevalence of 11 pathogenic genes of Campylobacter jejuni by PCR in strains isolated from humans, poultry meat and broiler and bovine faeces. J Med Microbiol 52: 345-348.

Hald B, Madsen M (1997) Healthy puppies and kittens as carriers of Campylobacter spp. with special reference to Campylobacter upsaliensis. J Clin Microbiol 35: 3351-3352.

Hald B, Pedersen K, Wainr M, Jrrgensen JC, Madsen M (2004) Longitudinal study of the excretion patterns of thermophilic Campylobacter spp. in young pet dogs in Denmark. J Clin Microbiol 24: 2003-2012.

Heywood W, Henderson B, Nair SP (2005) Cytolethal distending toxin: creating a gap in the cell cycle. J Med Microbiol 54: 207-216.

Hickey TE, McVeigh AL, Scott DA, Michielutti RE, Bixby A, Carroll SA, Bourgeois AL, Guerry P (2000) Campylobacter jejuni cytolethal distending toxin mediates release of interleukin-8 from intestinal epithelial cells. Infect Immun 68: 6535-6541.

Ketley JM (1997) Pathogenesis of enteric infection by Campylobacter. Microbiology 143: 5-21.

Koene MG, Houwers DJ, Dijkstra JR, Duim B, Wagenaar JA (2004) Simultaneous presence of multiple Campylobacter species in dogs. J Clin Microbiol 42: 819-821.

Konkel ME, Gray SA, Kim BJ, Gravis SG, Yoon J (1999) Identification of enteropathogenes Campylobacter jejuni and Campylobacter coli based on the cadF virulence gene and its product. J Clin Microbiol 37: 510-517.

de Lamballerie X, Zandotti C, Vignoli C, Bollet C, Micco P (1992) A one-step microbial DNA extraction method using "Chelex 100" suitable for gene amplification. Res Microbiol 143: 785-790.

Lin J (2009) Novel approaches for Campylobacter control in poultry. Foodborne Pathog Dis 6: 755-765.

Moore JE, Corcoran D, Dooley JS, Fanning S, Lucey B, Matsuda M, McDowell DA, Megraud F, Millar BC, O'Mahony R, O'Riordan L, O'Rourke M, Rao JR, Rooney PJ, Sails A, Whyte P (2005) Campylobacter. Vet Res 36: 351-382.

Moser I, Rieksneuwohner B, Lentzsch P, Schwerk P, Wieler LH (2001) Genomic heterogeneity and O-antigenic diversity of Campylobacter upsaliensis and Campylobacter helveticus strains isolated from dogs and cats in Germany. J Clin Microbiol 39: 2548-2557.

Nachamkin I, Bohachick K, Patton CM (1993) Flagellin gene typing of Campylobacter jejuni by restriction fragment length polymorphism analysis. J Clin Microbiol 31: 1531-1536.

On SL, Jordan PJ (2003) Evaluation of 11 PCR assays for species-level identification of Campylobacter jejuni and Campylobacter coli. J Clin Microbiol 41: 330-336.

Rizal A, Kumar A, Vidyarthi AS (2010) Prevalence of pathogenic genes in Campylobacter jejuni isolated from poultry and human. Internet Journal of Food Safety 12: 29-34.

Rożynek E, Dzierżanowska-Fangrat K, Jóźwiak P, Popowski J, Korsak D, Dzierżanowska D (2005) Prevalence of potential virulence markers in Polish Campylobacter jejuni and Campylobacter coli isolates obtained from hospitalized children and from chicken carcasses. J Med Microbiol 54: 615-619.

Smith JL, Bayles DO (2006) The contribution of cytolethal distending toxin to bacterial pathogenesis. Crit Rev Microbiol 32: 227-248.

Steinhauserova I, Fojtikova K, Klimes J (2000) The incidence and PCR detection of Campylobacter upsaliensis in dogs and cats. Lett Appl Microbiol 31: 209-212.

Talukder KA, Aslam M, Islam Z, Azmi IJ, Dutta DK, Hossain S, Nur-E-Kamal A, Nair GB, Cravioto A, Sack DA, Endtz HP (2008) Prevalence of virulence genes and cytolethal distending toxin in Campylobacter jejuni isolates from diarrheal patients. J Clin Microbiol 46: 1485-1488.

Wardak S, Szych J (2006) Prevalence of pathogenic genes of Campylobacter jejuni isolated from humans in Poland between 2003-2005. Med Dosw Mikrobiol 58: 217-222.

Wysok B, Uradziński J (2009) Campylobacter spp. - a significant microbiological hazard in food. I. Characteristics of Campylobacter species, infection source, epidemiology. Pol J Vet Sci 12: 141-148.

van Doorn PA (2009) What's new in Guillain-Barré syndrome in 2007-2008? J Peripher Nerv Syst 14: 72-74.

Wassenaar TM, Blaser MJ (1999) Pathophysiology of Campylobacter jejuni infections of humans. Microbes Infect 1: 1023-1033.

Polish Journal of Veterinary Sciences

The Journal of Committee of Veterinary Sciences of Polish Academy of Sciences and University of Warmia and Mazury in Olsztyn

Journal Information

IMPACT FACTOR 2016: 0.697
5-year IMPACT FACTOR: 0.773

CiteScore 2016: 0.73

SCImago Journal Rank (SJR) 2016: 0.315
Source Normalized Impact per Paper (SNIP) 2016: 0.486

Cited By


All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 210 132 5
PDF Downloads 88 66 8