Introduction. Electromagnetic radiation (EMR) that has an effect on living organisms may be a source of oxidative stress. A lack of proper compensation by antioxidant defences on the part of proteins leading to an uncontrolled growth of reactive forms of oxygen, which may give rise to numerous health conditions. Various scientific studies have indicated that propolis has multiple valuable medicinal properties: antibacterial, anti-inflammatory, antioxidant, protective - in relation to liver parenchyma, as well as anti-cancer. Nonetheless, the results of studies concerned with its antioxidant capabilities are not explicit and require further tests and analyses.
Objective. Determination of the effect of propolis supplementation on selected oxidative stress parameters in blood platelets exposed to electromagnetic radiation mitted by LCD monitors.
Material and methods. The material was a suspension containing human blood platelets. A 7% propolis solution was added to a cellular preparation before an exposure to EMR with a frequency of 1 kHz and an intensity of the electric component of 220 V/m (corresponding to sitting a distance of 15 cm from an LCD screen) for a total of 60 minutes. Before the exposure, as well as immediately following it, the researchers determined the level of activity of superoxide dismutase and malondialdehyde concentration, and evaluated it with regard to the control sample, i.e. material which was not subjected to EMR exposure.
Results. Electromagnetic radiation caused a statistically significant decrease in superoxide dismutase activity as compared with the reference group, irrespective of propolis supplementation. All of the studied groups indicated a minimal increase in the malondialdehyde concentration as compared with its initial values; however, the differences were not statistically significant.
Conclusions. The obtained results allow to conclude that the analysed electromagnetic radiation produced unfavourable changes in the activity of one of the enzymes responsible for antioxidative protection - superoxide dismutase (SOD - 1), and, moreover, a slight intensification in lipid peroxidation, which is expressed by an increase in malondialdehyde concentration (MDA). The supplementation with 7% propolis solution does not constitute sufficient protection against the negative effect of EMR. It is necessary to conduct further studies as well as determine the behaviour of other antioxidative enzymes.
1. Tichonov A. I. and others; Theory and practice in the production of medicinal propolis preparations. Apipol-Farma, Myślenice, 2004.
2. Kędzia B., Hołoderna-Kędzia E.: Chemical composition of propolis in the light of the hitherto executed research. Herba Pol., 1991, XXXVIII, 2, 95-110.
3. Kwakman PHS. and others: How honey kills bacteria, The FASEB Journal, 2010, 24, 2576-2582.
4. Castaldo S., Capasso F.: Propolis, an old remedy used in modern medicine. Fitoterapia, 2002, 73, Suppl.1, S1-S6.
5. Ozguner F, Bardak Y, Comlekci S. Protective effects of melatonin and caffeic acid phenethyl ester against retinal oxidative stress in long-term use of mobile phone: a comparative study. Mol Cell Biochem. 2006 Jan;282(1-2):83-8.
6. Balci M, Namuslu M, Devrim E, Durak I. Effects of computer monitor-emitted radiation on oxidant/antioxidant balance in cornea and lens from rats. Molecular Vision 2009; 15: 2521-2525.
7. Canseven A.G., Coskun S., Seyhan N., Effects of various extremely low frequency magnetic fields on the free radical processes, natural antioxidant system and respiratory burst system activities in the heart and liver tissues. Indian. J. Biochem. Biophys. 2008, Oct. 45(5): 326-31.
8. Röösli M, Egger M, Pfluger D, Minder C. Cardiovascular mortality and exposure to extremely low frequency magnetic fields: a cohort study of Swiss railway workers. Environ. Health. 2008; Jul 1;7: 35.
9. Bankowa V.: Chemical diversity of propolis and the problem of standardization. J Etnopharmacol., 2005, 100, 114-117.
10. Nakajima Y., Shimazawa M., Mishima S., Hara H. Water extract of propolis and its main constituents, caffeoylquinic acid derivatives, exert neuroprotective effects via antioxidant actions. Life Sci. 2007; 80: 370-377.
11. Demestre M and others CAPE (caffeic acid phenethyl ester)-based propolis extract (Bio 30) suppresses the growth of human neurofibromatosis (NF) tumor xenografts in mice. Phytother Res 2009; 23: 226-230.
12. Kumazawa S., Hamasaka T., Nakayama T.: Antioxidant activity of propolis of various geographic origins. Food Chem, 2004, 84, 329-339.
13. Li-Chang Lu, Yue-Wen Chen, Cheng-Chun Chou. Antibacterial and DPPH free radical scavenging activities of the ethanol extract of propolis collected in Taiwan. J. Food Drug Anal., 2003, Vol. 11, no 4, 277-282 J.
14. Shimizu K., Ashida H., Matsuura Y., Kanazawa K.: Antioxidative bioavailability of artepilin C in Brazilian propolis. Arch. Biochem. Biophy. 2004, 424, 181-188.
15. Capucho C, Sette R, de Souza Predes F, de Castro Monteiro J, Pigoso AA, Barbieri R, Dolder MA, Severi-Aguiar GD. Green Brazilian propolis effects on sperm count and epididymis morphology and oxidative stress. Food Chem Toxicol. 2012 Nov;50(11):3956-62.
16. Serra Bonvehi J., Ventura Coll F.: Study on Propolis Quality from China and Uruguay. Z. Naturforsch. 2000, 55c, 778-784.
17. Mika B. Tynka B. The comparison of bactericidal effect of various types of honey and propolis on gram-positive and gram-negative bacteria. Internet source: https://kbs.ise.polsl.pl/sknb/wp-content/.../Mika_Tynka-artykuł.pdf.
18. Kędzia B, Hołderna-Kędzia E. The elimination of harmful metals from an organism with the use of bee products. Herba polonica, 2009, 55,1: 95-108.
19. Shinohara R., Ohta Y., Hayashi T., Ikeno T.: Evaluation of antilipid peroxidative action of propolis ethanol extract. Phytother. Res., 2002, 16, 340-347.
20. Russo A., Longo R., Vanella A.: Antioxidant activity of propolis: role of caffeic acid phenethyl ester and galangin. Fitoterapia, 2002, 73, Suppl 1, 21- 29.
21. Lewicka M. and others Changes in the activity of superoxide dismutase in blood platelets exposed to electromagnetic radiation emitted by LCD screens - in vitro studies. Kwart. Ortop. 2011, 1, 31-37.
22. Koyu A. Ozguner F, Yilmaz H. The protective effect of caffeic acid phenethyl ester (CAPE) on oxidative stress in rat liver exposed to the 900 MHz electromagnetic field. Toxicol Ind Health. 2009 Jul;25(6):429-34.
23. Buczyński A., Pacholski K., Dziedziczak - Buczyńska and others; Alterations in the generation of free radicals in blood platelets exposed to electromagnetic radiation emitted by screen monitors; Polish Hyperbaric Research 2010; 1 (30).