Endosulfan Resistance Profile of Soil Bacteria and Potential Application of Resistant Strains in Bioremediation

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In the present study, bacterial strains were isolated from the soils of Wayanad District, Kerala, India and the isolates were tested for their tolerance to endosulfan and potential in bioremediation technology. Pesticide contamination in the soils, soil physico-chemical characteristics and socio-economic impacts of pesticide application were also analyzed. 28 pesticide compounds in the soil samples were analyzed and the results revealed that there was no pesticide residues in the soils. As per the survey conducted the pesticide application is very high in the study area and the level of awareness among the farmers was very poor regarding the method of application and its socio-economic and ecological impacts. A total of 9 bacterial strains were isolated with 50μg/ml of endosulfan in the isolating media and the results showed that most of the bacterial strains were highly resistance to endosulfan. Out of the 9 strains isolated 6 were highly resistant to endosulfan (500- 700μg/ml) and the other 3 isolates showed the resistance of 250-500μg/ml. From the studied isolate, isolate 9 demonstrating prolific growth and high resistance was selected to check their capability to degrade endosulfan over time. Identification of the selected strain reveals that it belongs to the genus Bacillus. Results of endosulfan removal studies showed that with increase in time, the biomass of the bacterial strains increased. The complete disappearance of endosulfan from the spiked and inoculated broth during the first day of incubation (24 hour interval) was observed. While the control flask showed the presence of endosulfan during the experimental period. Pesticide resistant bacteria are widely distributed in the soils of selected study area and the tolerance varied between bacteria even though they were isolated from the soils of the same area. The selected Bacillus species carry the ability to degrade endosulfan at accelerated rates and it could be useful in framing a bioremediation strategy for pesticide contaminated soil and water environments

Ajayi, O.C., Camara, M., Fleischer, G., Haidara, F., Sow, M., Traore, A., Van Der Valk, H. (2002). Socio-economic assessment of pesticide use in Mali. Pesticide Policy Project [Special Issue]. Publication series no. 6. Germany: University of Hanover.

Andreu, V., Pico, Y. (2004). Determination of pesticides and their degradation products in soil: critical review and comparison of methods. Trends in Analytical Chemistry, 23, 10-11.

Antle, J.M., Pingali, P.L. (1995). Pesticides, productivity, and farmer health: a Philippine case study. In P. L. Pingali, & P. A. Roger (Eds.), Impact of pesticides on farmer health and the rice environment. Philippines: International Rice Research Institute. 361-385.

Awasthi, N., Manickam, N., Kumar, A. (1997). Biodegradation of endosulfan by a bacterial coculture. Bulletin of Environmental Contamination and Toxicology, 59, 928-934.

Barnes, B.V., Zak, D.R., Denton, S.R., Spurr, S.H. (1998). (eds.). Forest Ecology. Fourth ed., New York. (pp 74) Wiley.

Bavcon, M., Trebse, P., Zupancic-Kralj, L. (2002). Investigations of the determination and transformations of diazinon and malathion under environmental conditions using gas chromatography coupled with a flame ionization detector. Chemosphere, 50, 595-601.

Brubaker, S.C., Jones, A.J., Lewis, D.T., Frank, K. (1993). Soil properties associated with landscape position. Soil Science Society of America Journal, 57, 235-239.

Ferrer, M.R., Gonzalez-Lopez, J., Ramos-Cormenzana, A. (1986). Effect of some herbicides on the biological activity of Azotobacter vinelandii. Soil Biology and Biochemistry, 18, 237-238.

Greipsson, S., El-Mayas, H. (1999). Large-scale reclamation of barren lands in Iceland by aerial seeding. Land Degradation and Development,10,185 -193.

Greipsson, S. (1995). Effect of iron plaque on roots of rice on growth of plants in excess zinc and accumulation of phosphorus in plants in excess copper and nickel. Journal of Plant Nutrition, 18, 1659 -1665.

Holt, J.G., Kreig, N.R., Sneath, P.H.A., Staley, J.T., Williams, S.T. (1994). Bergey’s Manual of Determinative Bacteriology, 9th edn. Baltimore, Maryland 21202, USA: Williams and Wilkins.

Hussain, S., Arsad, M., Saleem, M., Kalid, A. (2007). Biodegradation of alpha and beta endosulfan by soil bacteria. Biodegradation, 1, 120-138.

Jackson, M.H. (1973). Soil chemical analysis .Prentice hall of India (Pvt Ltd), New Delhi, India.

Kathpal, T.S., Singh, A., Dhankhar, J.S., Singh, G. (1997). Fate of endosulfan in cotton soil under sub-tropical conditions of Northern India. Pesticide Science, 50, 21-27.

Keith, L.H., Telliard, W.A. (1979). Priority pollutants I: A perspective view. Environmental Science and Technology, 13, 416-423.

Kullman, S.W., Matsumura, F. (1996). Metabolic pathway utilized by Phanerochaete chrysosporium for degradation of the cyclodiene pesticide endosulfan. Applied and Environmental Microbiology, 62, 593-600.

Lakshmi, C.V., Kumar, M., Khanna, S. (2009). Biodegradation of Chlorpyrifos in Soil by Enriched Cultures. Current Microbiology, 58, 35-38.

Lui, G.W., Talnagi, J.W., Strohl, W.R., Pfister, R.M. (1983). Hexavalent chromium resistant bacteria isolated from river sediments. Applied Environmental Microbiology, 46, 846-854.

Macrae, I.C. (1990). The microbial Degradation of pesticides and related compounds. Residue Reviews, 88, 187.

Mancini, F., Van Bruggen, A.H.C., Jiggins, J.L.S., Ambatipudi, A.C., Murphy, H. (2005). Acute pesticide poisoning among female and male cotton growers in India. International Journal of Occupational and Environmental Health, 11, 221-232.

Maumbe, B.M., Swinton, S.M. (2003). Hidden health costs of pesticide use in Zimbabwe’s smallholder cotton growers. Social Science & Medicine, 57, 1559-1571.

Morgan, M.F. (1945). Chemical diagnoses by the universal soil testing system. Connectieut agricultural experiment station, SA, 450.

Murphy, H.H., Sanusi, A., Dilts, R., Djajadisastra, M., Hirschhorn, N., Yuliantiningsih, S. (1999). Health effects of pesticide use among Indonesian women farmers: part I: exposure and acute health effects. Journal of Agromedicine, 6, 61-85.

Pimentel, D. (2005). Environmental and economic costs of the application of pesticides primarily in the United States. Environment, Development and Sustainability. 7, 229-252.

Racke, K.D., (1990). Pesticides in the soil microbial ecosystem. Enhanced biodegradation of pesticides in the environment. In: Racke, K.D., Coats, J.R. (Eds.), Enhanced Biodegradation of Pesticides in the Environment. American Chemical Society, Washington, DC, 1-12.

Rao, D.M.R., Murty, A.S. (1980). Persistence of endosulfan in soils. Journal of Agricultural and Food Chemistry, 28, 1099-1101.

Sassman, S.A., Lee, L.S., Bischoff, M., Turco, R.F., 2004. Assessing N,N’-dibutylurea (DBU) formation in soils after application of n-butylisocyanate and benlate fungicides. Journal of Agricultural and Food Chemistry, 52, 747.

Scowcroft, P.G., Turner, D.R., Vitousek, P.M. (2006). Decomposition of Metrosideros polymorpha leaf litter along elevational gradients in Hawaii. Global Change Biology, 6, 73-85.

Singh, K., Brajesh, K., Walker, A., Alum, J., Morgan, W., Wright, D.J., 2004. Biodegradation of chlorpyrifos by Enterobacter strain B-14 and its use in biodegradation of contaminated soils. Applied Environmental Microbiology, 70, 4855-4863.

Sparks, R. (2003). Environmental Soil Chemistry, Elsevier, Amsterdam, The Netherlands.

Stewart, D.K.R., Cairns, K.G. (1974). Endosulfan persistence in soil and uptake by potato tubers. Journal of Agricultural and Food Chemistry, 22, 984-986.

Sunderam, R.I.M., Cheng, D.M.H. (1992). Toxicity of endosulfan to native and introduced fish in Australia. Environmental Toxicology & Chemistry, 11, 1469-1476.

Verschueren, K. (1983). Endosulfan. In: Handbook of Environmental Data on Organic Chemicals. Van Nostrand Reinhold, New York, 604-607.

Wilson, C., Tisdell, C. (2001). Why farmers continue to use pesticides despite environmental, health and sustainability costs. Ecological Economics, 39, 449-462.

Wilson, C. (2000). Environmental and human costs of commercial agricultural production in south Asia. International Journal of Social Economics, 27, 816-846.

Yassin, M.M., Abu Mourad, T.A., Safi, J.M. (2002). Knowledge, attitude, practice, and toxicity symptoms associated with pesticide use among farm workers in the Gaza Strip. Occupational and Environmental Medicine, 59, 387-394.

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