Mineralization of teeth and bones of the cave bear (Ursus spelaeus) from the Biśnik Cave, Southern Poland

Open access

Mineralization of teeth and bones of the cave bear (Ursus spelaeus) from the Biśnik Cave, Southern Poland

The studied bones and teeth of the cave bear (Ursus spelaeus) come from the Biśnik Cave, located in the Częstochowa Upland (Southern Poland). The specimens originate from different geological layers formed since the Odra Glaciation (250-270 thousand years BP). The fossilized bones and teeth were studied using optical microscopy, scanning electron microscopy, X-ray diffraction, FTIR spectroscopy, and INAA. They are built of recrystallized carbonate-rich apatite-(CaOH) and/or apatite-(CaOH). The teeth additionally contain some apatite-(CaF). The lack of collagen and minor REE contents suggest rapid burial and collagen decay in the early stage of diagenesis. The bones and teeth have only limited mineral infillings. In some teeth, Mn-Fe (hydroxy)oxides were found in the dentine canaliculi and in bones, some osteocyte lacunae contain Fe (hydroxy)oxides with admixture of Mn. In one bone specimen, calcite infillings are present in Haversian canals. The infillings formed during later stages of diagenesis and were succeeded by non-filled cracks.

Bell L. S. (1990). Paleopathology and diagenesis: An SEM evaluation of structural changes using backscattered electron imaging. Journal of Archaeological Science, 17, 85-102.

Belouafa S., Chaair H., Loukili H., Digua K., & Sallek B. (2008). Characterization of antiseptic apatite powders prepared at biomimetics temperature and pH. Materials Research, 11(1), 93-96. DOI: 10.1590/S1516-14392008000100018

Bocherens H., Brinkam D. B., Dauphin Y., & Mariotti A. (1994). Microstructural and geochemical investigations on late Cretaceous archosaur teeth from Alberta, Canada. Canadian Journal of Earth Science, 31, 783-792.

Brady A. C., White C. D., Longstaffe F. J., & Southham G. (2008). Investigating intra-bone isotopic variations in bioapatite using IR- laser ablation and micromilling: Implications for identifying diagenesis? Palaeogeography, Palaeoclimatology, Palaeoecology, 266, 190-199. DOI: 10.1016/j.palaeo.2008.03.031.

Calafiori A. R., Marotta M., Nastro A., & Martino G. (2004). Low temperature method for the production of calcium phosphate fillers. BioMedical Engineering Online, 3, 8. DOI: 10.1186/1475-925X-3-8.

Cyrek K., Mirosław-Grabowska J., Stefaniak K., & Socha P. (2009). Archaeology, stratigraphy and palaeoecology of the Biśnik Cave. In K. Stefaniak P. Socha & A. Tyc (Eds.), Karst of the Częstochowa Upland and the Eastern Sudetes - Palaeoenvironments and Protection (pp. 77-98). Sosnowiec-Wrocław, Poland: Top Art.

Dauphin Y., & Williams C. T. (2004). Diagenetic trends of dental tissues. Comptes Rendus Palevol, 3(6-7), 583-590. DOI: 10.1016/j.crpv.2004.07.007.

Denys C., Wiliam C. T., Dauphin Y., Andrews P., & Fernandez-Jalvo Y. (1996). Diagenetical changes in Pleistocene small mammals bones from Olduvai Bed-1. Palaeogeography, Palaeoclimatology, Palaeoecology, 126, 121-134.

Elliott J. C. (2002). Calcium phosphate biominerals. Reviews in Mineralogy and Geochemistry, 48, 427-453. DOI: 10.2138/rmg.2002.48.11.

Elorza J., Astibia H., Murelaga X., & Pereda-Suberbiola X. (1999). Francolite as a diagenetic mineral in dinosaur and other Upper Cretaceous reptile bones (Laño, Iberian Peninsula): microstructural, petrological and geochemical features. Cretaceous Research, 20, 169-187. DOI: 10.1006/cres.1999.0144.

Fleet M. E., & Liu X. (2004). Location of type B carbonate ion in type A-B carbonate apatite synthesized at high pressure. Journal of Solid State Chemistry, 177, 3174-3182. DOI: 10.1016/j.jssc.2004.04.002.

Garland A. N. (1987). Paleohistology. Science and Archaecology, 29, 25-29.

Garland A. N. (1989). Microscopical analysis of fossil bone. Applied Geochemistry, 4, 215-229.

Gross K. A. & Berndt C. C. (2002). Biomedical Application of Apatites. Reviews in Mineralogy and Geochemistry, 48, 631-673. DOI: 10.2138/rmg.2002.48.17

Gutiérrez M. (2001). Bone diagenesis and taphonomic history of the Paso Otero 1 bone bed, Pampas of Argentina. Journal of Archaeological Science, 28, 1277-1290. DOI: 10.1006/jasc.2000.0648

Gutiérrez-Salazar M., & Reyes-Gasga J. (2003). Microhardness and chemical composition of human tooth. Material Research, 6, 1-7. DOI: 10.1590/S1516-14392003000300011

Hancock R. G. V., Grynpas M. D., & Pritzker K. P. H. (1989). The abuse of bone analyses for archeological dietary studies. Archaeometry, 31, 169-179.

Hedges R. E. M., Millard A. R., & Pike A. W. G. (1995). Measurements and relationships of diagenetic alteration of bone from three archaeological sites. Journal of Archaeological Science, 22, 201-209.

Hoffman E. L. (1992). Instrumental neutron activation in geoanalysis. Journal of Geochemical Exploration, 44, 297-319.

Hubert J. F., Panish P. T., Chure D. J., & Prostak K. S. (1996). Chemistry, microstructure, petrology, and diagenetic model of Jurassic dinosaur bones, Dinosaur National Monument, Utah. Journal of Sedimentary Research, 66, 531-547.

Jacques L., Ogle N., Moussa I., Kalin R., Vignaud P., Brunet M., & Bocherens H. (2008). Implications of diagenesis for the isotopic analysis of Upper Miocene large mammalian herbivore tooth enamel from Chad. Palaeogeography, Palaeoclimatology, Palaeoecology, 266, 200-210. DOI: 10.1016/j.palaeo.2008.03.040.

Kohn M. J., Schoeninger M. J., & Valley J. W. (1996). Herbivore tooth oxygen compositions: Effects on diet and physiology. Geochimica et Cosmochimica Acta, 60, 3889-3896. DOI: 10.1016/0016-7037(96)00248-7.

Kohn M. J., Schoeninger J., & Barker W. W. (1999). Altered states: Effect on fossil tooth chemistry. Geochimica et Cosmochimica Acta, 63, 2737-2747. DOI: 10.1016/S0016-7037(99)00208-2.

Longinelli A. (1983). Oxygen isotopes in mammal bone phosphate: a new tool for paleohydrological and paleoclimatological research? Geochimica et Cosmochimica Acta, 48, 385-390.

Mirosław-Grabowska J. (1998). Stratygrafia osadów czwartorzędowych wschodniej części Pasma Smoleńsko-Niegowonickiego (Wyżyna Krakowsko-Częstochowska). Studia Geologica Polonica, 113, 105-119.

Mirosław-Grabowska J. (2002). Litologia i stratygrafia osadów Jaskini Biśnik. In K. Cyrek (Ed.), Jaskinia Biśnik. Rekonstrukcja zasiedlenia jaskini na tle zmian środowiska przyrodniczego (pp. 143-179). Toruń, Poland: Wydawnictwo Uniwersytetu Mikołaja Kopernika.

National Cancer Institute (2009). Compact Bone and Spongy (Cancellous Bone). Retriéed November 6, 2009, from http://training.seer.cancer.gov/images/anatomy/skeletal/bone_tissue.jpg

Nielsen-Marsh C. M. (1997). Studies in Archaeological Bone Diagenesis. Unpublished doctoral thesis, University of Oxford, United Kingdom.

Nielsen-Marsh C. M., & Hedges R. E. M. (2000). Patterns of diagenesis in bones I: The effects of site environments. Journal of Archaeological Science, 27, 1139-1150. DOI: 10.1006/jasc. 1999.0537.

Palmqvist P., Gröcke D. R., Arribas A., & Fariña R. A. (2003). Paleoecological reconstruction of a lower Pleistocene large mammal community using biogeochemical (δ13C, δ15N, δ18O, Sr: Zn) and ecomorphological approaches. Paleobiology, 29, 205-229. DOI: 10.1666/0094-8373(2003)029<0205:PROALP>2.0.CO;2.

Person A., Bocherens H., Saliege J. F., Paris F., Zeitoun V., & Gerard M. (1995). Early diagenetic evolution of bone phosphate: an X-ray diffractometry analysis. Journal of Archaeological Science 22, 211-221.

Pike A. W. G. (1993). Bone Porosity, Water and Diagenesis: Towards a Grand Unified Theory of Bone Diagenesis. Unpublished Bachelor Thesis. University of Bradford, United Kingdom.

Pfretzschner H. U. (2000). Microcracks and fossilization of Haversian bone. Neue Jahrbuch für Geologie und Paläontologie Abhandlungen, 216, 413-431.

Pfretzschner H. U. (2004). Journal of Archaeological Science: Fossilization of Haversian bone in aquatic environments. Comptes Rendus Palevol, 3, 605-616. DOI: 10.1016/j.crpv.2004.07.006.

Reiche I., Favre-Quattropani L., Calligaro T., Salomon J., Bocherens H., Charlet L., & Menu M. (1999). Trace element composition or archaeological bones and postmortem alteration in the burial environment. Nuclear Instruments and Methods in Physics Research, 150, 656-662. DOI: 10.1016/S0168-583X(98)00949-5.

Rey C., Collins B., Goehl T., Dickson R., & Glimsher M. J. (1989). The carbonate environment in bone mineral: A resolution-enhanced Fourier transform infrared spectroscopy study. Calcified Tissue International, 45, 157-164.

Rink W. J., & Schwarcz H. P. (1995). Tests for diagenesis in tooth enamel: ESR dating signals and carbonate contents. Journal of Archaeological Science, 22, 251-255.

Sillen A., & Sealy J. C. (1995). Diagenesis of strontium in fossil bone: A reconsideration of Nelson et al. (1986). Journal of Archaeological Science, 22, 313-320. DOI: 10.1006/jasc.1995.0033.

Simmer J. P., & Fincham A. G. (1995). Molecular mechanism of dental enamel formation. Critical Reviews in Oral Biology & Medicine, 6, 84-108. DOI: 10.1177/10454411950060020701.

Skinner H. C. W. (2000). In praise of phosphates, or why vertebrates chose apatite to mineralize their skeletons. International Geological Review, 42, 232-240.

Sønju Clasen A. B, & Ruyter I. E. (1997). Quantitative determination of type A and type B carbonate in human deciduous and permanent enamel by means of Fourier Transform Infrared Spectrometry. Advances in Dental Research, 11, 523-527. DOI: 10.1177/08959374970110042101.

Sukhodub L. F., Moseke C., Sukhodub L. B., Sulkio-Cleff B., Maleev V. Ya., Semenov M. A., Bereznyak E. G., & Bolbukh T. V. (2004). Collagen-hydroxyapatite-water interactions investigated by XRD, piezogravimetry, infrared and Raman spectroscopy. Journal of Molecular Structure, 704, 53-58. DOI: 10.1016/j.molstruc.2003.12.061.

Trueman C. N., & Tuross N. (2002). Trace elements in recent and fossil bone apatite. Reviews in Mineralogy and Geochemistry, 48, 489-521. DOI: 10.2138/rmg.2002.48.13.

Trueman C. N., Behrendsmeyer A. K., Tuross N., & Weiner S. (2004). Mineralogical and compositional changes in bones exposed on soil surfaces in Amboseli National Park, Kenya: diagenetic mechanism and the role of sediment pore fluids. Journal of Archaeological Science, 31, 721-739. DOI: 10.1016/j.jas.2003.11.003.

Tuross N., Behrensmeyer A. K., & Eanes E. D. (1989). Strontium increases and crystallinity changes in taphonomic and archaeological bone. Journal of Archaeological Science, 16, 661-672.

Tütken T. (2003). Die Bedeutung der Knochenfrühdiagenese für die Erhaltungsfähigkeit in vivo erworbener Elementund Isotopenzusammensetzungen in fossilen Knochen. Unpublished doctoral dissertation, University of Tübingen, Germany.

Tütken T., Pfretzschner H. U., Vennemann T. W., Sun G., & Wang Y. D. (2004). Paleobiology and skeletochronology of Jurassic dinosaurs: implications from the histology and oxygen isotope compositions of bones. Palaeogeography, Palaeoclimatology, Palaeoecology, 206, 217-238. DOI: 10.1016/j.palaeo.2004.01.005.

Tütken T., Vennemann T. W., Janz H., & Heizmann E. P. J. (2006). Palaeoenvironment and palaeoclimate of the Middle Miocene lake in the Steinheim basin, SW Germany: A reconstruction from C, O, and Sr isotopes of fossil remains. Palaeogeography, Palaeoclimatology, Palaeoecology, 241, 457-491. DOI: 10.1016/j.palaeo.2006.04.007.

Tütken T., Furrer H., & Vennemann T. W. (2007). Stable isotope compositions of mammoth teeth from Niederweningen, Switzerland: Implications for the Late Pleistocene climate, environment and diet. Quaternary International, 164-165, 139-150. DOI: 10.1016/j.quaint.2006.09.004.

Tütken T., Vennemann T. W., & Pfretzschner H. U. (2008). Early diagenesis of bone and tooth apatite in fluvial and marine settings: Constraints from combined oxygen isotope, nitrogen and REE analysis. Palaeogeography, Palaeoclimatology, Palaeoecology, 266, 254-268. DOI: 10.1016/j.palaeo.2008.03.037.

Wings O. (2004). Authigenic minerals in fossil bones from the Mesozoic of England: poor correlation with depositional environments. Palaeogeography, Palaeoclimatology, Palaeoecology, 204, 15-32. DOI: 10.1016/S0031-0182(03)00709-0.

Wiszniowska T., Socha P., & Stefaniak K. (2002). Czwartorzędowa fauna z osadów Jaskini Biśnik. In K. Cyrek (Ed.), Jaskinia Biśnik. Rekonstrukcja zasiedlenia jaskini na tle zmian środowiska przyrodniczego, 192-220. Toruń, Poland: Wydawnictwo Uniwersytetu Mikołaja Kopernika.

Wiśniewski M., Sionkowska A., Kaczmarek H., Lazare S., & Tokarev V. (2007). Wpływ promieniowania laserowego na cienkie błony kolagenowe (Influence of laser irradiation on the thin collagen films). Polimery, 52, 571-578.

Wopenka B., & Pasteris J. D. (2005). A mineralogical perspective on the apatite in bone. Materials Science and Engineering: C., 25, 131-143. DOI: 10.1016/j.msec.2005.01.008.

Wychowański P., Kolmas L., Kalinowski E., Krzywicki D., Chomicki P., Gąsiorowska M., Wojtowicz A., & Kołodziejski W. (2006). Analiza porównawcza szkliwa i zębiny ludzkich zębów prawidłowych i nadliczbowych metodą mikrospetroskopii w zakresie średniej podczerwieni. Dental and medical problems, 43, 53-57.


The Journal of Mineralogical Society of Poland

Journal Information

CiteScore 2017: 0.82

SCImago Journal Rank (SJR) 2017: 0.272
Source Normalized Impact per Paper (SNIP) 2017: 0.342


All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 250 250 21
PDF Downloads 78 78 7