Introduction:Mycoplasma synoviae (MS) is a chicken pathogen of major economic importance.
Material and Methods: Between 2010 and 2016, 906 commercial layer chicken flocks in Poland were examined for MS, and the phylogenetic relationship among the strains was established. Regionally dispersed samples were collected and tested with the use of real-time PCR to detect the 16S–23S intergenic spacer region. Positive samples were also tested with LAMP and conventional PCR to detect the vlhA gene.
Results: MS genetic material was detected in 265 (29%) of the tested flocks by real-time PCR, in 227 by the LAMP method and in 202 (22%) by conventional PCR. The by-year percentage of positive samples began at 34% in 2010, rose to 44% in 2012, and declined to 29% in 2016. A phylogenetic analysis of Polish M. synoviae strains using a partial sequence of the vlhA gene showed nine genotypes (A–I), the most frequently occurring being F and C. Pathogenic Polish MS field isolates (n = 27) collected from chickens with clinical signs of infection were grouped for their characteristic symptoms: respiratory for genotypes C, E, F, and I (n = 13), EAA and a drop in laying for genotypes F, E, and C (n = 12), and synovitis for genotype A (n = 2).
Conclusion: These data showed the country’s isolate diversity. The high prevalence suggests the need to introduce appropriate control programmes. This is the first report of molecular epidemiological data on M. synoviae infection in layer chickens in Poland.
1. Benčina D., Drobnič-Valič M., Horvat S., Narat M., Kleven S.H., Dovč P.: Molecular basis of the length variation in the N-terminal part of Mycoplasma synoviae hemagglutinin. FEMS Microbiol Lett 2001, 203, 115–123.
2. Berčič R.L., Slavec B., Lavrič M., Narat M., Bidovec A., Dovč P., Benčina D.: Identification of major immunogenic proteins of Mycoplasma synoviae isolates. Vet Microbiol 2008, 127, 147–154.
3. Catania S., Bilato D., Gobbo F., Granato A., Terregino C., Iob L., Nicholas R.A.J.: Treatment of eggshell abnormalities and reduced egg production caused by Mycoplasma synoviae infection. Avian Dis 2010, 54, 961–964.
4. Catania S., Gobbo F., Bilato D., Gagliazzo L., Moronato M.L., Terregino C., Bradbury J.M., Ramírez A.S.: Two strains of Mycoplasma synoviae from chicken flocks on the same layer farm differ in their ability to produce eggshell apex abnormality. Vet Microbiol 2016, 193, 60–66.
5. Dijkman R., Feberwee A., Landman W.J.M.: Variable lipoprotein haemagglutinin (vlhA) gene sequence typing of mainly Dutch Mycoplasma synoviae isolates: comparison with vlhA sequences from Genbank and with amplified fragment length polymorphism analysis. Avian Pathol 2014, 43, 465–472.
6. Dufour-Gesbert F., Dheilly A., Marois C., Kempf I.: Epidemiological study on Mycoplasma synoviae infection in layers. Vet Microbiol 2006, 114, 148–154.
7. El-Gazzar M.M., Wetzel A.N., Raviv Z.: The genotyping potential of the Mycoplasma synoviae vlhA gene. Avian Dis 2012, 56, 711–719.
8. Feberwee A., De Vries T.S., Landman W.J.M.: Seroprevalence of Mycoplasma synoviae in Dutch commercial poultry farms. Avian Pathol 2008, 37, 629–633.
9. Feberwee A., De Wit J.J., Landman W.J.M.: Induction of eggshell apex abnormalities by Mycoplasma synoviae: field and experimental studies. Avian Pathol 2009, 38, 77–85.
11. Frey M.L., Hanson R.P., Anderson D.P.: A medium for the isolation of avian mycoplasmas. Am J Vet Res 1968, 29, 2163–2171.
12. Gole V.C., Chousalkar K.K., Roberts J.R.: Prevalence of antibodies to Mycoplasma synoviae in laying hens and possible effects on egg shell quality. Prev Vet Med 2012, 106, 75–78.
13. Hagan J.C., Ashton N.J., Bradbury J.M., Morgan K.L.: Evaluation of an egg yolk enzyme-linked immunosorbent assay antibody test and its use to assess the prevalence of Mycoplasma synoviae in UK laying hens. Avian Pathol 2004, 33, 93–97.
14. Hammond P.P., Ramírez A.S., Morrow C.J., Bradbury J.M.: Development and evaluation of an improved diagnostic PCR for Mycoplasma synoviae using primers located in the haemagglutinin encoding gene vlhA and its value for strain typing. Vet Microbiol 2009, 136, 1–2.
15. Harada K., Kijima-Tanaka M., Uchiyama M., Yamamoto T., Oishi K., Arao M., Takahashi T.: Molecular typing of Japanese field isolates and live commercial vaccine strain of Mycoplasma synoviae using improved pulsed-field gel electrophoresis and vlhA gene sequencing. Avian Dis 2009, 53, 538–543.
16. Hong Y., Garcia M., Leiting,V., Benčina D., Dufour-Zavala L., Zavala G., Kleven S.H.: Specific detection and typing of Mycoplasma synoviae strains in poultry with PCR and DNA sequence analysis targeting the hemagglutinin encoding gene vlhA. Avian Dis 2004, 48, 606–616.
17. Jeon E.O., Kim J.N., Lee H.R., Koo B.S., Min K.C., Han M.S., Lee S.B., Bae Y.J., Mo J.S., Cho S.H., Lee C.H., Mo I.P.: Eggshell apex abnormalities associated with Mycoplasma synoviae infection in layers. J Vet Sci 2014, 15, 579–582.
18. Kleven S.H.: Mycoplasmas in the etiology of multifactorial respiratory disease. Poultry Sci 1998, 77, 1146–1149.
19. Kohn S., Spergser J., Ahlers C., Voss M., Bartels T., Rosengarten R., Krautwald-Junghanns M.E. Prevalence of Mycoplasmas in commercial layer flocks during laying period. Berl Munch Tierarztl Wochenschr 2009, 122, 186–192.
25. Lockaby S.B., Hoerr F.J., Lauerma L.H., Kleven S.H.: Pathogenicity of Mycoplasma synoviae in broiler chickens. Vet Pathol 1998, 35, 178–190.
26. Moreira F.A., Cardoso L., Coelho A.C.: Epidemiological survey on Mycoplasma synoviae infection in Portuguese broiler breeder flock. Vet Ital 2015, 51, 93–98.
27. Noormohammadi A.H., Markham P.F., Kanci A., Whithear K.G., Browning G.F.: A novel mechanism for control of antigenic variation in the haemagglutinin gene family of Mycoplasma synoviae. Mol Microbiol 2000, 35, 911–923.
28. Ogino S., Munakata Y., Ohashi S., Fukui M., Sakamoto H., Sekiya Y., Noormohammadi A.H., Morrow C.J.: Genotyping of Japanese field isolates of Mycoplasma synoviae and rapid molecular differentiation from the MS-H vaccine strain. Avian Dis 2011, 55, 187–194.
29. Ranck M.F., Schmidt V., Philipp H.C., Voss M., Kacza J., Richter A., Fehlhaber K., Krautwald-Junghanns M.E.: Mycoplasma synoviae-associated egg-pole shell defects in laying hens. Berl Munch Tierarztl Wochenschr 2010, 123, 111–118.
30. Raviv Z., Kleven S.H.: The development of diagnostic real-time TaqMan PCRs for the four pathogenic avian mycoplasmas. Avian Dis 2009, 53, 103–107.
31. Razin S., Yogev D., Naot Y.: Molecular biology and pathogenicity of mycoplasmas. Microbiology and molecular biology reviews. MMBR 1998, 62, 1094–1156.
32. Strugnell B.W., McMullin P., Wood A.M., Nicholas R.A.J., Ayling R., Irvine R.M.: Unusual eggshell defects in a free-range layer flock in Great Britain. Vet Rec 2011, 169, 237–238.
33. Sun S., Lin X., Liu J., Tian Z., Chen F., Cao Y., Qin J., Luo T.: Phylogenetic and pathogenic analysis of Mycoplasma synoviae isolated from native chicken breeds in China. Poult Sci 2017, 96, 2057–2063.