Ameliorative effects of Helianthus annuus against nephrotoxic, cardiac, and haematological disorders in alloxan-induced hyperglycaemia in albino rats

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Introduction: The study evaluated the ameliorative effects of Helianthus annuus leaf extract on nephrotoxicity, cardiac, and haematologic disorders in alloxan-induced hyperglycaemic rats.

Material and Methods: The cold maceration method with 80% methanol was used in the preparation of H. annuus extract. Thirty alloxan-induced hyperglycaemic rats were randomly assigned to five equal groups (A–E). Groups A and B received 5% tween-20 solution in water (5 mL/kg) and glibenclamide (2 mg/kg), respectively; while groups C, D, and E received 150, 300, and 600 mg/kg of the extract, respectively, per os once daily for 21 consecutive days. The levels of serum urea, creatinine, haematological indices, and histopathological changes in the kidneys and heart were evaluated 24 h after the last treatment on day 21.

Results: The extract and glibenclamide significantly (P < 0.05) reduced the levels of serum urea and urea : creatinine ratio in diabetic rats when compared with the vehicle treated group. The extract and glibenclamide also ameliorated haematological disorders and kidney and cardiac damage induced by alloxan.

Conclusion: H. annuus extract produced nephroprotective, cardioprotective, and haematoprotective effects and might prevent the advancement of diabetic complications such as diabetic nephropathy and cardiovascular diseases in diabetic patients.

1. Babitt J.L., Lin H.,Y.: Mechanisms of anemia in CKD. J Am Soc Nephrol 2012, 23, 1631–1634.

2. Bekkevold C.M., Robertson K.L., Reinhard M.K., Battles A.H., Rowland N.E.: Dehydration parameters and standards for laboratory mice. J Am Assoc Lab Anim Sci 2013, 52, 233–239.

3. Besarab A., Levin A.: Defining a renal anemia management period. Am J Kidney Dis 2000, 36, 13–23.

4. Brar R.S., Sandhu H.S., Singh A.: Veterinary clinical diagnosis by laboratory methods. Kalyani Publishers, New Delhi, 2000.

5. Chen L., Yin H., Lan Z., Ma S., Zhang C., Yang Z., Li P., Lin B.: Anti-hyperuricemic and nephroprotective effects of Smilax china L. J Ethnopharmacol 2011, 135, 399–405.

6. Creno R.J., Wenk R.E., Bohlig P.: Automated micro-measurement of urea using urease and the Berthelot reaction. Am J Clin Pathol 1970, 54, 828–832.

7. D’alessandro A., Kriebardis A.G., Rinalducci S., Antonelou M.H., Hansen K.C., Papassideri I.S., Zolla L.: An update on red blood cell storage lesions, as gleaned through biochemistry and omics technologies. Transfusion 2014, 55, 205–219.

8. Ejebe D.E., Siminialayi I.M., Emudainohwo J.O.T., Ofesi U., Morka L.: Analgesic and anti-inflammatory activities of the ethanol extract of the leaves of Helianthus annuus in Wistar rats. Asian Pac J Trop Med 2010, 3, 341–347.

9. Ejebe D.E., Siminialayi I.M., Nwadito C., Emudainowho J.O.T., Akonghrere R., Ovuakporaye S.I.: Effects of ethanol extract of leaves of Helianthus annuus on the reproductive system of male Wistar rats: Testicular histology, epididymal sperm properties, and blood levels of reproductive hormones. Biomed Pharmacol J 2008, 1, 65–78.

10. Elsner M., Tiedge M., Guldbakke B., Munday R., Lenzen S.: Importance of the GLUT-2 glucose transporter for pancreatic beta cell toxicity of alloxan. Diabetologia 2002, 45, 1542–1549.

11. Ene A.C., Nwankwo E.A., Samdi L.M.: Alloxan-induced diabetes in rats and the effects of black Caraway (Carum carvi L.) oil on their body weights. J Toxicol Pharmacol 2008, 3, 141–146.

12. Esser N., Legrand-Poels S., Piette J., Scheen A.J., Paquot N.: Inflammation as a link between obesity, metabolic syndrome, and type 2 diabetes. Diabetes Res Clin Pract 2014, 105, 141–150.

13. Ezeja M.I., Anaga A.O., Asuzu I.U.: Acute and sub-chronic toxicity profile of methanol leaf extract of Gouania longipetala in rats. J Ethnopharmacol 2014, 151, 1155–1164.

14. Forbes J.M., Cooper M.E.: Mechanisms of diabetic complications. Physiol Rev 2013, 93, 137–188.

15. Grossmann V., Schmitt V.H., Zeller T., Panova-Noeva M., Schulz A., Laubert-Reh D., Juenger C., Schnabel R.B., Abt T.G., Laskowski R., Wiltink J.: Profile of the immune and inflammatory response in individuals with prediabetes and type 2 diabetes. Diabetes Care 2015, 38, 1356–1364.

16. Hua J., Huang P., Zhu C., Yuan X., Yu C.: Anti-hyperuricemic and nephroprotective effects of modified Simiao decoction in hyperuricemic mice. J Ethnopharmacol 2012, 142, 248–252.

17. Kishore L., Kaur N., Singh R.: Nephroprotective effect of Paeonia emodi via inhibition of advanced glycation end products and oxidative stress in streptozotocin–nicotinamide induced diabetic nephropathy. J Food Drug Anal 2017, 25, 576–588.

18. Le K., Beaufrère H., Bassel L.L., Wills S., Laniesse D., Blois S.L., Smith D.A.: Diagnosis and management of polycythemia vera in a ferret (Mustela putorius furo). Comp Med 2016, 66, 463–467.

19. Marketon J.I.W., Glaser R.: Stress hormones and immune function. Cell Immunol 2008, 252, 16–26.

20. McKinney A.M.: Dense vessels simulating thrombosis on non-enhanced CT. In: Atlas of Normal Imaging Variations of the Brain, Skull, and Craniocervical Vasculature Cham, Springer International Publishing, London, 2017, pp. 1307–1319,

21. Means R.T.: Recent developments in the anemia of chronic disease. Curr Hematol Rep 2003, 2, 116–121.

22. Muller D.N., Kvakan H., Luft F.C.: Immune-related effects in hypertension and target-organ damage. Curr Opin Nephrol Hypertens 2011, 20, 113–117.

23. Najjar F.M., Taghavi F., Ghadari R., Sheibani N., Moosavi-Movahedi A.A.: Destructive effect of non-enzymatic glycation on catalase and remediation via curcumin. Arch Biochem Biophys 2017, 630, 81–90.

24. Nazmi A.S., Ahmad S.J., Pillai K.K., Akhtar M., Ahmad A., Najmi A.K.: Protective effects of Bombyx mori, quercetin and benazepril against doxorubicin induced cardiotoxicity and nephrotoxicity. J Saudi Chem Soc 2016, 20, 573–578.

25. Oliva L., Baron C., Fernández-López J., Remesar X., Alemany M.: Marked increase in rat red blood cell membrane protein glycosylation by one-month treatment with a cafeteria diet. 2015.

26. Onoja S.O., Anaga A.O.: Evaluation of the antidiabetic and antioxidant potentials of methanolic leaf extract of Helianthus annuus L. on alloxan-induced hyperglycemic rats. Comp Clin Pathol 2014, 23, 1565–1573.

27. Panda S., Kar A., Sharma P., Sharma A.: Cardioprotective potential of N,α-l-rhamnopyranosyl vincosamide, an indole alkaloid, isolated from the leaves of Moringa oleifera in isoproterenol induced cardiotoxic rats: In vivo and in vitro studies. Bioorg Med Chem Lett 2013, 23, 959–962.

28. Pourghasem M., Nasiri E., Shafi H.: Early renal histological changes in alloxan-induced diabetic rats. Int J Mol Cell Med 2014, 3, 11–15.

29. Scheen A.J., Esser N., Paquot N.: Antidiabetic agents: Potential anti-inflammatory activity beyond glucose control. Diabetes Metab 2015, 41, 183–194.

30. Ship J.A., Fischer D.J.: The relationship between dehydration and parotid salivary gland function in young and older healthy adults. J Gerontol A Biol Sci Med Sci 1997, 52A, 310–319.

31. Slot C.: Plasma creatinine determination. A new and specific Jaffe reaction method. Scand J Clin Lab Invest 1965, 17, 381–387.

32. Sundararajan R., Bharampuram A., Koduru R.: A review on phytoconstituents for nephroprotective activity. Pharmacophore 2014, 5, 160–182.

33. Thrall M.A., Weiser G., Allison R., Campbell T.W.: Veterinary Haematology and Clinical Chemistry. John Wiley and Sons, London, 2012.

34. Tomaiuolo G.: Biomechanical properties of red blood cells in health and disease towards microfluidics. Biomicrofluidics 2014, 8,

35. Yankuzo H., Ahmed Q.U., Santosa R.I., Akter S.F.U., Talib N.A.: Beneficial effect of the leaves of Murraya koenigii (Linn.) Spreng (Rutaceae) on diabetes-induced renal damage in vivo. J Ethnopharmacol 2011, 135, 88–94.

Journal of Veterinary Research

formerly Bulletin of the Veterinary Institute in Pulawy

Journal Information

IMPACT FACTOR J Vet Res 2017: 0.811

CiteScore 2017: 0.68

SCImago Journal Rank (SJR) 2017: 0.29
Source Normalized Impact per Paper (SNIP) 2017: 0.484


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