Effects of repeated lipopolysaccharide treatment on growth performance, immune organ index, and blood parameters of Sprague-Dawley rats

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Abstract

Introduction: The study was designed to investigate the effects of repeated lipopolysaccharide (LPS) treatment on growth performance, lymphoid organ indexes, and blood cells in Sprague-Dawley rats.

Material and Methods: Forty healthy weaned Sprague-Dawley rats were randomly equally divided into LPS and control groups. Each rat in the LPS group was injected via the caudal vein with LPS (100 μg/kg b.w.) for 10 days, and the control group was treated with an equal volume of normal saline. On the 1st, 4th, 7th, and 10th days, growth performance, lymphoid organ indexes, and blood cells were evaluated in five necropsied rats.

Results: When rats were treated 3–10 times with LPS, their body weight and average daily gains increased more slowly than in the control group (P < 0.05). Repeated LPS treatment significantly increased spleen weight and the ratio of spleen to body weight (P < 0.05). White blood cells, neutrophils, and neutrophil percentage increased (P < 0.05) remarkably, but lymphocyte percentage, haemoglobin, and blood platelet counts decreased significantly (P < 0.05).

Conclusion: LPS treatment obviously suppresses growth and promotes peripheral immune organ proliferation. It is indicated that host protective mechanism can be activated by multiple small doses of LPS and prevents organs from further damage during stress status.

1. Campos P.H., Merlot E., Damon M., Noblet J., Le Floc’h N.: High ambient temperature alleviates the inflammatory response and growth depression in pigs challenged with Escherichia coli lipopolysaccharide. Vet J 2014, 200, 404–409.

2. Cesta M.F.: Normal structure, function, and histology of the spleen. Toxicol Pathol 2006, 34, 455–465.

3. Faas M.M., Moes H., van der Schaaf G., de Leij L.F., Heineman M.J.: Total white blood cell counts and LPS-induced TNF alpha production by monocytes of pregnant, pseudopregnant, and cyclic rats. J Reprod Immunol 2003, 59, 39–52.

4. Hayley S., Mangano E., Strickland M., Anisman H.: Lipopolysaccharide and a social stressor influence behaviour, corticosterone, and cytokine levels: divergent actions in cyclooxygenase-2 deficient mice and wild type controls. J Neuroimmunol 2008, 197, 29–36.

5. Huang H., Liu A., Wu H., Ansari A.R., Wang J., Huang X., Zhao X., Peng K., Zhong J., Liu H.: Transcriptome analysis indicated that Salmonella lipopolysaccharide-induced thymocyte death and thymic atrophy were related to TLR4-FOS/JUN pathway in chicks. BMC Genomics 2016, 17, 322.

6. Johnson R.W.: Inhibition of growth by pro-inflammatory cytokines: an integrated view. J Anim Sci 1997, 75, 1244–1255.

7. Kaplanski J., Fraifeld V., Rubin M.: Body temperature and hypothalamic PGE2 response to LPS in developing rats. Ann N Y Acad Sci 1997, 813, 474–479.

8. Kim M.H., Yun C.H., Kim G.R., Kol J.Y., Lee J.J., Ha J.K.: Changes of immunoglobulins and lymphocyte subpopulations in peripheral blood from Holstein calves challenged with Escherichia coli lipopolysaccharide. Asian Austral J Anim 2011, 24, 696–706.

9. Lukasiewicz J., Lugowski C.: Biologic activity of lipopolysaccharides. Postepy Hig Med Dosw 2003, 57, 33–53.

10. Myers M.J., Farrell D.E., Palmer D.C., Post L.O.: Inflammatory mediator production in swine following endotoxin challenge with or without co-administration of dexamethasone. Int Immunopharmacol 2003, 3, 571–579.

11. Nishikawa T., Omura M., Kawaguchi M., Takatsu A., Satoh F., Ito S., Kurihara I., Itoh H., Yanase T., Shibata H., Oki Y., Naruse M., Sakurai K., Sasamoto H., Kuwa K.: Calibration and evaluation of routine methods by serum certified reference material for aldosterone measurement in blood. Endocrinol J 2016, 63, 1065–1080.

12. Obernikhin S.S., Yaglova N.V.: Morphological and functional changes in the thymus and spleen of mouse offspring in the development of systemic inflammatory response after a single immunity stimulation in early pregnancy. Bull Exp Biol Med 2014, 157, 812–815.

13. Olson N.C., Hellyer P.W., Dodam J.R.: Mediators and vascular effects in response to endotoxin. Br Vet J 1995, 151, 489–522.

14. Pomorska-Mól M., Czyżewska-Dors E., Kwit K., Pejsak Z.: Enrofloxacin in therapeutic doses alters cytokine production by porcine PBMCs induced by lipopolysaccharide. Drug Chem Toxicol 2017, 40, 295–299.

15. Qazi M.R., Bogdanska J., Butenhoff J.L., Nelson B.D., DePierre J.W., Abedi-Valugerdi M.: High-dose, short-term exposure of mice to perfluorooctanesulfonate (PFOS) or perfluorooctanoate (PFOA) affects the number of circulating neutrophils differently, but enhances the inflammatory responses of macrophages to lipopolysaccharide in a similar fashion. Toxicology 2009, 262, 207–214.

16. Reddan D.N., Klassen P.S., Szczech L.A., Coladonato J.A., O’Shea S., Owen W.J., Lowrie E.G.: White blood cells as a novel mortality predictor in haemodialysis patients. Nephrol Dial Transplant 2003, 18, 1167–1173.

17. Revathy N.S.: Routine measurements of cord arterial blood lactate levels in infants delivering at term and prediction of neonatal outcome. Med J Malaysia 2016, 71, 131–133.

18. Ronco C.: Lipopolysaccharide from the cellular wall of Gram-negative bacteria, also known as endotoxin, is a key molecule in the pathogenesis of sepsis and septic shock. Blood Purificat 2014, 37, 1.

19. Schmidhammer R., Wassermann E., Germann P., Redl H., Ullrich R.: Infusion of increasing doses of endotoxin induces progressive acute lung injury but prevents early pulmonary hypertension in pigs. Shock 2006, 25, 389–394.

20. Seydel U., Hawkins L., Schromm A.B., Heine H., Scheel O., Koch M.H., Brandenburg K.: The generalized endotoxic principle. Eur J Immunol 2003, 33, 1586–1592.

21. Spurlock M.E.: Regulation of metabolism and growth during immune challenge: an overview of cytokine function. J Anim Sci 1997, 75, 1773–1783.

22. Tesh V.L., Morrison D.C.: The physical-chemical characterization and biologic activity of serum released lipopolysaccharides. J Immunol 1988, 141, 3523–3531.

23. Van Balveren J.A., Huijskens M.J., Gemen E.F., Pequeriaux N.C., Kusters R.: Effects of time and temperature on 48 routine chemistry, haematology, and coagulation analytes in whole blood samples. Ann Clin Biochem 2016, 54, 448–462.

24. Webel D.M., Finck B.N., Baker D.H., Johnson R.W.: Time course of increased plasma cytokines, cortisol, and urea nitrogen in pigs following intraperitoneal injection of lipopolysaccharide. J Anim Sci 1997, 75, 1514–1520.

25. Zhang N., Li H., Jiang C., Tu Y., Diao Q.: Effects of lipopolysaccharide on the growth performance, nitrogen metabolism and immunity in preruminant calves. Indian J Anim Res 2017, 51, 717–721.

26. Zhao L., Chen Y.H., Wang H., Ji Y.L., Ning H., Wang S.F., Zhang C., Lu J.W., Duan Z.H., Xu D.X.: Reactive oxygen species contribute to lipopolysaccharide-induced teratogenesis in mice. Toxicol Sci 2008, 103, 149–157.

Journal of Veterinary Research

formerly Bulletin of the Veterinary Institute in Pulawy

Journal Information


IMPACT FACTOR J Vet Res 2017: 0.811

CiteScore 2017: 0.68

SCImago Journal Rank (SJR) 2017: 0.29
Source Normalized Impact per Paper (SNIP) 2017: 0.484

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