Introduction: One aim of the study was to evaluate the impact when added to feed of the two potentially probiotic strains of lactic acid bacteria (LAB) Lactobacillus plantarum K KKP 593/p and Lactobacillus rhamnosus KKP 825 on production performance, health, and the composition of gut microbiota. The complementary aim was to assess the safety of these strains in broiler rearing.
Material and Methods: A total of 500 one-day-old Ross 308 chicks were divided into four groups. The experimental factor was the admixture of bacterial preparation to the feed at different doses: the recommended maximum dose, a dose ten times higher, the recommended minimum dose, and a zero dose for the control group not receiving bacteria.
Results: Addition of bacteria to the diets did not have a significant effect on the final body weight, final body weight gain, nor total feed intake or feed conversion. However, lactic acid bacteria had a positive effect on chicken health. Mortality among chickens fed with LAB was reduced. Moreover, LAB feeding inhibited the growth of Salmonella spp. and Clostridium perfringens in the intestines. There were no significant differences in chicken performance by dose of bacteria in the feed. The group dosed with LAB ten times higher than the recommended maximum did not demonstrate changes in biochemical or haematological parameters of blood compared to the remaining groups.
Conclusion: Feeding chicken broilers with two potentially probiotic LAB strains is safe and impacts animal health positively.
1. Abdi-Hachesoo B., Talebi A., Asri-Rezaei S., Basaki M.: Sex related differences in biochemical and hematological parameters of adult indigenous chickens in Northwest of Iran. J Anim Sci Adv 2013, 3, 512–516.
2. Albokhadaim I., Althnainan T., El-Bahr S.: Investigation of selected biochemical parameters of local chickens with different age and sex in Al-ahsa, Saudi Arabia. Pakistan J Biol Sci 2012, 15, 827–832.
3. Arslan M., Ozcan M., Matur E., Coteliogliu U., Ergul E.: The effect of vitamin E on some blood parameters in broilers. Turk J Vet Anim Sci 25, 2001, 711–716.
4. Binek M., Kizerwetter-Świda M., Cisek A., Rzewuska M., Chrobak-Chmiel D., Gieryńska M.: Mechanisms of maintenance of intestinal homeostasis by autochthonic microbiota and probiotics. Med Weter 2016, 72, 611-615. doi: 10.21521/mw.5575.
5. Brzóska F., Buluchevskij S., Stecka K., Śliwiński B.: The effects of lactic acid bacteria and mannan oligosaccharide, with or without fumaric acid, on chicken performance, slaughter yield and digestive tract microflora. J Anim Feed Sci 2007, 16, 241–251.
6. Clinical Diagnostic Division. Veterinary reference guide: A summary of reference intervals for use with KODAK EKTACHEM products, 1990, Rochester (NY): Eastman Kodak Company.
7. EFSA Scientific opinion. Technical guidance. Tolerance and efficacy studies in target animals. EFSA J 2011, 9, 15, doi:10.2903/j.efsa.2011.2175.
8. Grela E., Kowalczyk-Pecka D., Hanczakowska E., Matras J.: Effect of inulin and a probiotic supplement in the diet of pigs on selected traits of the gastrointestinal microbiome. Med Weter 2016, 72, 448–452.
9. Harr K.: Clinical chemistry of companion avian species: A review. Vet Clin Path, 2002, 31, 140–151.
10. Harr K.: Diagnostic value of biochemistry. In: Clinical avian medicine. Volume II, edited by Spix Publishing Inc., Palm Beach, USA, 2005, pp. 612–630.
11. Hrnčár C., Weis J., Mindek S., Bujko J.: Effect of probiotic addition in drinking water on body weight and body measurements of broiler chickens. Anim Sci Biotechnol 2014, 47, 249–253.
12. Kaceci T., Col R.: Haematological and biochemical values of the blood of pheasants (Phasianus colchicus) of different ages. Turk J Vet Anim Sci 2011, 35, 149–156, doi:10.3906/vet-0910-13.
13. Klebaniuk R., Grela E., Kowalczuk-Vasilev E., Florek M., Góźdź J., Pecka S., Danek-Majewska A.: Wpływ ekologicznych dodatków ziołowych w żywieniu zwierząt na ich zdrowotność. In: Wyniki badań z zakresu rolnictwa ekologicznego w 2011 roku. Ministerstwo Rolnictwa i Rozwoju Wsi, 2011, Warszawa, ISBN: 978-83-62178-52-0.
14. Lan R., Lee S., Kim I.: Effects of Enterococcus faecium SLB 120 on growth performance, blood parameters, relative organ weight, breast muscle meat quality, excreta microbiota shedding and noxious gas emission in broilers. Poult Sci 2017, 96, 3246–3253.
16. Murshed M., Abudabos A.: Effects of the dietary inclusion of a probiotic, a prebiotic or their combinations on the growth performance of broiler chickens. Rev Brasil Ciencia Avicola 2015, 17, 99–104.
17. Olnood C., Beski S., Choct M., Iji P.: Novel probiotics: their effects on growth performance, gut development, microbial community and activity of broiler chickens. Anim Nutr 2015, 1, 184–191.
18. Olnood C., Beski S., Iji P., Choct M.: Delivery routes for probiotics. Effects on broiler performance, intestinal morphology and gut microflora. Anim Nutr 2015, 1, 192–202.
19. Olnood C., Beski S., Choct M., Iji P.: Use of Lactobacillus johnsonii in broilers challenger with Salmonella sofia. Anim Nutr 2015, 1, 203–212.
20. Patyra E., Kwiatek K.: Probiotic feed additives. Med Weter 2013, 69, 225–230.
21. Scientific Opinion on the update of the list of QPS-recommended biological agents intentionally added to food or feed as notified to EFSA. EFSA J 2017, 15, 4664.
22. Seifi K., Torshizi M., Rahimi S., Kazemifard M.: Efficiency of early, single-dose probiotic administration methods on performance, small intestinal morphology, blood biochemistry, and immune response of Japanese quail. Poult Sci 2017, 96, 2151–2158.
23. Simaraks S., Chinrasri O., Aengwanic S.: Haematological, electrolyte and serum biochemical values of the Thai indigenous chicken (Gallus domesticus) in Northeastern Thailand. Song Klanakarin J Sci Technol 2004, 26, 425–430.
24. Timmerman H., Veldman A., van den Elsen E., Rombouts F., Beynen A.: Mortality and growth performance of broilers given drinking water supplemented with chicken-specific probiotics. Poultry Sci 2010, 85, 1383–1388.