Introduction: Phagocytic activity and oxygen metabolism of peripheral blood granulocytes from rabbits with experimental trichophytosis were assessed by flow cytometry.
Material and Methods: Virulent species of T. mentagrophytes var. granulosum (Tm-K) isolated from rabbits with natural trichophytosis was used for experimental infection. The phagocytic activity of granulocytes was measured in whole blood by flow cytometry using the commercial Phagotest kit. Oxidative burst was measured in whole blood by flow cytometry using the commercial Bursttest kit.
Results: It was found that rabbits were susceptible to infection with Trichophyton mentagrophytes under experimental conditions. The analysis of the phagocytic activity indices and oxygen metabolism of granulocytes in peripheral blood of infected rabbits showed that changes of the indices were connected with the progression and regression of the disease. A significant decrease in phagocytic activity and oxygen metabolism was observed during development of fungal lesions and it remained similar throughout the progress of the disease. The highest means of the percentage of activated and ingesting phagocytes and a significant increase in the mean fluorescence intensity (representing the number of ingested bacteria) were observed during spontaneous recovery. Therefore, the decrease or increase in the indices of phagocytic activity and oxygen metabolism of granulocytes from rabbits experimentally infected with T. mentagrophytes is somehow related to the progress of infection and suppressive activity of the fungus, whose elimination during recovery caused significant increases in investigated indices of non-specific cellular immunity.
Conclusion: The results of the present investigation confirm that the mechanism of oxygen-dependent killing is crucial in infections caused by T. mentagrophytes.
1. Banks K.L., Clarkson T.B.: Naturally occurring dermatomycoses in rabbit. J Am Vet Med Assoc 1967, 151, 926–929.
2. Cabrera R.M., Blake J.S., Dahl M.V.: Inhibition of keratinocyte proliferation by a mannan glycoprotein isolated from Trichophyton rubrum. J Invest Dermatol 1991, 96, 616–621.
3. Cafarchia C., Weigl S., Figueredo L.A., Otranto D.: Molecular identification and phylogenesis of dermatophytes isolated from rabbit farms and rabbit farm workers. Vet Microbiol 2012, 154, 395–402.
4. Calderon R.A., Hay R.J.: Fungicidal activity of human neutrophils and monocytes on dermatophyte fungi, Trichophyton quinckeanum and Trichophyton rubrum. Immunology 1987, 61, 289–295.
5. Dahl M.V.: Suppression of immunity and inflammation by products produced by dermatophytes. J Am Acad Dermatol 1993, 28, 19–23.
6. Davies R.R., Zaini F.: Trichophyton rubrum and the chemotaxis of polymorphonuclear leukocytes. J Med Vet Mycol 1984, 22, 65–71.
7. Ergin C., İlkit M., Gök Y., Özel M.Z., Çon A.H., Kabay N., Söyleyici S., Döğen A.: Fourier transform infrared spectral evaluation for the differentiation of clinically relevant Trichophyton species. J Microbiol Methods 2013, 93, 218–223.
8. Grando S.A., Herron M.J., Dahl M.V.: Binding and uptake of Trichophyton rubrum mannan by human epidermal keratinocytes: a time course study. Acta Dermatol Venerol 1992, 72, 273–276.
9. Grando S.A., Hostager B.S., Herron M.J.: Binding of Trichophyton rubrum mannan to human monocytes in vitro. J Invest Dermatol 1992, 98, 876–880.
10. Gregurek-Novak T.: Effect of infection with Trichophyton mentagrophytes varietas interdigitale on phagocytosis in humans. Eur Acad Dermatol Venereol 2004, 18, 160–163.
11. Hagen K.W.: Ringworm in domestic rabbits: oral treatment with griseofulvin. Lab Anim Care 1969, 19, 635–638.
12. Hau C., Tada Y., Kanda N., Watanabe S.: Immunoresponses in dermatomycoses. J Dermatol 2015, 42, 236–244.
13. Kostro K., Gliński Z.: The kinetics of the immune response to natural trichophytosis in arctic foxes. Folia Vet 2000, 44, 106–111.
14. Kostro K., Gliński Z., Wojcicka-Lorenowicz K., Gacek L., Krakowski M.: Allopevac vaccine in prophylactics and controlling trichophytosis in rabbits. Med Weter 2000, 56, 816–821.
15. Kostro K., Gliński Z., Wojcicka-Lorenowicz K.: Immunological responses in dermatomycoses of animals. Med Weter 2001, 57, 709–714.
16. Kostro K., Gliński Z., Wojcicka-Lorenowicz K., Nozdryn-Płotnicki Z.: Antiinfectious immunity of skin. Med. Weter 2002, 58, 655–660.
17. Kraemer A., Mueller R.S., Werckenthin C., Straubinger R.K., Hein J.: Dermatophytes in pet guinea pigs and rabbits. Vet Microbiol 2012, 157, 208–213.
18. LeibundGut-Landmann S., Wuthrich M., Hohl T.M.: Immunity to fungi. Current Opinion Immunol 2012, 24, 449–458.
19. Mesquita J.R., Vasconcelos-Nóbrega C., Oliveira J., Coelho C., Vala H., Fratti M., Arabatzis M., Velegraki A., Monod M.: Epizootic and epidemic dermatophytose outbreaks caused by Trichophyton mentagrophytes from rabbits in Portugal 2015. Mycoses 2016, 59, 668–673.
20. Symoensa F., Joussonb O., Planarda C., Frattic M., Staibd P., Mignone B., Monod M.: Molecular analysis and mating behaviour of the Trichophyton mentagrophytes species complex. Int J Med Microbiol 2011, 301, 260–266.
22. Szili M., Kohalmi I.: Endemic Trichophyton mentagrophytes infection of rabbit origin. Mycosen 1981, 24, 412–420.
23. Wojcicka-Lorenowicz K., Kostro K.: Flow cytometry evaluation of phagocytic activity and oxygen metabolism of granulocytes in peripheral blood of rabbits with natural trichophytosis. Bull Vet Inst Pulawy 2008, 52, 555–560.
24. Wojcicka-Lorenowicz K., Kostro K., Taszkun I.: Specific cell-mediated immune responses in rabbits naturally and experimentally infected with Trichophyton mentagrophytes and in immunised animals. Bull Vet Inst Pulawy 2013, 57, 329−333.
25. Wołoszyn S., Umiński M.: Serological and allergic activity of native and pure trichophytin. Med Weter 1985, 41, 394−398.