Analytical strategy for determination of chloramphenicol in different biological matrices by liquid chromatography - mass spectrometry

Open access

Abstract

Introduction: The main problem in determination of chloramphenicol in food of animal origin is a large number of matrices. The main target of this study was to create a method for determination and confirmation of chloramphenicol in products and food of animal origin. Material and Methods: Each 5 g matrix sample was mixed with 5 mL of water and 10 mL of acetonitrile/ethyl acetate, homogenised, and centrifuged. The organic layer was evaporated and redissolved in 6 mL of 4% NaCl. The extract was cleaned up by SPE technique. Chloramphenicol was analysed by LC-MS/MS in electrospray mode. Results: The procedure was validated according to the Commission Decision No. 2002/657/EC. The apparent recoveries were in the range of 92.1% to 107.1% with a repeatability less than 11.0% (4.4%-11.0%) and within-laboratory reproducibility below 13.6% (4.7%-13.6%). Conclusion: The method was successfully validated and proved to be efficient, precise, and useful for quantification of chloramphenicol in more than 20 different matrices.

1. Berendsen B.J., Zuidema T., de Jong J., Stolker L.A., Nielen M.W.: Discrimination of eight chloramphenicol isomers by liquid chromatography tandem mass spectrometry in order to investigate the natural occurrence of chloramphenicol. Anal Chim Acta 2011, 700, 78-85.

2. Commission Decision 2002/657/EC of 12 August 2002 implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results. OJ L 221, 17.8.2002, 8-36.

3. Guy P.A., Royer D., Mottier P., Gremaud E., Perisset A., Stadler R.H.: Quantitative determination of chloramphenicol in milk powders by isotope dilution liquid chromatography coupled to tandem mass spectrometry. J Chromatogr A 2004, 1054, 365-371.

4. Kittler K., Radeck W., Polzer J.: Investigations on the influence of hydrolysis on the total amount of marker residue and consequences. Conference Material, EuroResidue VIII, in Egmond aan Zee, the Netherlands 2016. pp. 129-131.

5. Nicolich R.S., Werneck-Barroso E., Marques M.A.S.: Food safety evaluation: Detection and confirmation of chloramphenicol in milk by high performance liquid chromatography-tandem mass spectrometry. Anal Chim Acta 2006, 565, 97-102.

6. RASFF - the Rapid Alert System for Food and Feed. https://webgate.ec.europa.eu/rasff-window/portal/?event=searchResultList.

7. Rejtharová M., Rejthar L.: Determination of chloramphenicol in urine, feed water, milk, and honey samples using molecular imprinted polymer clean-up. J Chromatogr A 2009, 1216, 8246-8253.

8. Rezende D.R., Filho N.F., Rocha G.L. Simultaneous determination of chloramphenicol and florfenicol in liquid milk, milk powder, and bovine muscles by LC-MS/MS. Food Addit Contam A 2012, 29, 559-570.

9. Rocha Siqueira S.R., Luiz Donato J., de Nucci G., Reyes, F.G.R.: A high-throughput method for determining chloramphenicol residues in poultry, egg, shrimp, fish, swine, and bovine using LC-ESI-MS/MS. J Sep Sci 2009, 32, 4012-4019.

10. Rønning H.T., Einarsen K., Asp T.N.: Determination of chloramphenicol residues in meat, seafood, egg, honey, milk, plasma, and urine with liquid chromatography-tandem mass spectrometry, and the validation of the method based on 2002/657/EC. J Chromatogr A 2006, 1118, 226-233.

11. Shen J., Xia X., Jiang H., Li C., Li J., Li X., Ding S.: Determination of chloramphenicol, thiamphenicol, florfenicol, and florfenicol amine in poultry andporcine muscle and liver by gas chromatography-negative chemical ionization mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 2009, 877, 1523-1529.

12. Sniegocki T., Posyniak A., Gbylik-Sikorska M., Zmudzki J.: Determination of chloramphenicol in milk using a QuEChERSbased on liquid chromatography tandem mass spectrometry Method Anal Letters 2014, 47, 568-578.

13. Sniegocki T., Posyniak A., Zmudzki J.: Determination of chloramphenicol residues in milk by gas and liquid chromatography mass spectrometry methods. Bull Vet Inst Pulawy 2007, 51, 59-64.

14. Sniegocki T., Gbylik-Sikorska M., Posyniak A.: Transfer of chloramphenicol from milk to commercial dairy products - experimental proof. Food Control 2015, 57, 411-418.

15. Tajik H., Malekinejad H., Razavi-Rouhani S.M., Pajouhi M.R., Mahmoudi R., Haghnazari A.: Chloramphenicol residues in chicken liver, kidney and muscle: a comparison among the antibacterial residues monitoring methods of Four Plate Test, ELISA and HPLC. Food Chem Toxicol 2010, 48, 2464-2468.

16. Vivekanandan K., Swamy M. G., Prasad S., Mukherjee R.: A simple method of isolation of chloramphenicol in honey and its estimation by liquid chromatography coupled to electrospray ionization tandem mass spectrometry. Rapid Commun Mass Spectrom 2005, 19, 3025-3030.

Journal of Veterinary Research

formerly Bulletin of the Veterinary Institute in Pulawy

Journal Information


IMPACT FACTOR J Vet Res 2017: 0.811

CiteScore 2017: 0.68

SCImago Journal Rank (SJR) 2017: 0.29
Source Normalized Impact per Paper (SNIP) 2017: 0.484

Metrics

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 296 296 23
PDF Downloads 130 130 10