Detection of avian reoviruses in wild birds in Poland

Open access


Introduction: The purpose of this study was to determine the occurrence of avian reovirus (ARV) infections in wild birds in Poland and attempt to propagate the selected ARV strains in chicken embryo kidney (CEK) cells or chicken SPF embryos. Material and Methods: The study included 192 wild birds representing 32 species, collected between 2014 and 2016. A part of the S4 segment encoding the σNS protein of avian reoviruses (ARVs) isolated from different species of wild birds from that period was amplified. Results: The presence of ARV was demonstrated in 58 (30.2%) wild birds belonging to nine orders. The isolated strains were propagated in chicken embryos by yolk sac inoculation, and CPE was induced in the infected CEK monolayer. Agar gel precipitation showed that two ARV isolates from rock pigeon and mute swan shared a common groupspecific antigen with chicken reovirus S1133. Specific products of predicted size were found in two ARV isolates from the chicken embryo passage and 13 ARVs isolated from CEK cells. Conclusion: The study indicates the high prevalence of ARV among wild birds in Poland and its possible transmission to farmed birds.

1. Bányai K., Dandár E., Dorsey K.M., Mató T., Palya V.: The genomic constellation of a novel avian orthoreovirus strain associated with runting-stunting syndrome in broilers. Virus Genes 2011, 42, 82-89.

2. Benavente J., Martínez-Costas J.: Avian reovirus: structure and biology. Virus Res 2007, 123, 105-119.

3. Bruhn S., Bruckner L., Ottiger H.P.: Application of RT-PCR for the detection of avian reovirus contamination in avian viral vaccines. J Virol Methods 2005, 123, 179-186.

4. Hollmen T., Docherty D.E.: Orthoreovirus. In: Infectious Diseases of Wild Birds, edited by Thomas N.C., Hunter D.B., Atkinson C.T.: Blackwell Publishing, Iowa, USA, 2007, pp. 177-181.

5. Huhtamo E., Uzcátegui N.Y., Manni T., Munsterhjelm R., Brummer-Korvenkontio M., Vaheri A., Vapalahti O.: Novel orthoreovirus from diseased crow, Finland. Emerg Infect Dis 2007, 13, 1967-1969.

6. Jones R.C.: Reovirus infections. In: Diseases of Poultry, edited by Saif Y.M. et al., Blackwell, USA, 2008, pp. 309-328.

7. Jones R.C., Guneratne J.R.: The pathogenicity of some avian reoviruses with particular reference to tenosynovitis. Avian Pathol 1984, 13, 173-189.

8. Ke G.M., Cheng H.L., Ke L.Y., Ji W.T., Chulu J.L., Liao M.H., Chang T.J., Liu H.J.: Development of a quantitative light cycler real-time RT-PCR for detection of avian reovirus. J Virol Methods 2006, 133, 6-13.

9. Kugler R., Dandár E., Fehér E., Jakab F., Mató T., Palya V., Bányai K., Farkas S.L.: Phylogenetic analysis of a novel reassortant orthoreovirus strain detected in partridge (Perdix perdix). Virus Res 2016, 215, 99-103.

10. Lawson B., Dastjerdi A., Shah S., Everest D., Núñez A., Pocknell A., Hicks D., Horton D.L., Cunningham A.A., Irvine R.M.: Mortality associated with avian reovirus infection in a free-living magpie (Pica pica) in Great Britain. BMC Vet Res 2015, 11, 20, doi:

11. Liu H.J., Lee L.H., Hsu H.W., Kuo L.C., Liao M.H.: Molecular evolution of avian reovirus: evidence for genetic diversity and reassortment of the S-class genome segments and multiple cocirculating lineages. Virology 2003, 314, 336-349.

12. Liu Q., Zhang G., Huang Y., Ren G., Chen L., Gao J., Zhang D., Han B., Su W., Zhao J., Hu X., Su J.: Isolation and characterization of a reovirus causing spleen necrosis in Pekin ducklings. Vet Microbiol 2011, 148, 200-206.

13. Lu H., Tang Y., Dunn P.A., Wallner-Pendleton E.A., Lin L., Knoll E.A.: Isolation and molecular characterization of newly emerging avian reovirus variants and novel strains in Pennsylvania, USA, 2011-2014. Sci Rep 2015, 5, 1-11.

14. Martínez-Costas J., Grande A., Varela R., García-Martínez C., Benavente J.: Protein architecture of avian reovirus S1133 and identification of the cell attachment protein. J Virol 1997, 71, 59-64.

15. Matthews R.: The classification and nomenclature of viruses: summary of results of meetings of the International Committee on Taxonomy of Viruses in Strasbourg, August 1981. Intervirology 1981, 16, 53-60.

16. Mor S.K., Armién A.G., Reed L., Schott R., Goyal S.M.: Detection and molecular characterization of a reovirus in blackcapped chickadees (Poecile atricapillus) from Minnesota, USA. J Wildl Dis 2014, 50, 928-932.

17. Mutlu O.F., Grund C., Cöven F.: Reovirus infection of pheasants (Phasianus colchicus). Tierarztl Prax Ausg G Grosstiere Nutztiere 1998, 2, 104-107.

18. Ogasawara Y., Ueda H., Kikuchi N., Kirisawa R.: Isolation and genomic characterization of a novel orthoreovirus from a browneared bulbul (Hypsipetes amaurotis) in Japan. J Gen Virol 2015, 96, 777-786.

19. Samorek-Salamonowicz E., Minta Z.: Zakażenia reowirusami (reovirus infections). In: Choroby drobiu, edited by Mazurkiewicz M., Wroclaw, 2011, pp. 464-470.

20. Spandidos D.A., Graham A.F.: Physical and chemical characterization of an avian reovirus. J Virol 1976, 19, 968-976.

21. Van der Heide L.: The history of avian reovirus. Avian Dis 2000, 44, 638-641.

22. Van Der Heide L.: Viral arthritis/tenosynovitis: a review. Avian Pathol 1977, 6, 271-284.

23. Woźniakowski G., Samorek-Salamonowicz E., Gaweł A.: Occurrence of reovirus infection in Muscovy ducks (Cairina moschata) in south western Poland. Pol J Vet Sci 2014, 17, 299-305.

24. Zhang Y., Liu M., Shuidong O., Hu Q.L., Guo D.C., Chen H.Y., Han Z.: Detection and identification of avian, duck, and goose reoviruses by RT-PCR: goose and duck reoviruses are part of the same genogroup in the genus Orthoreovirus. Arch Virol 2006, 151, 1525-1538.

Journal of Veterinary Research

formerly Bulletin of the Veterinary Institute in Pulawy

Journal Information

IMPACT FACTOR J Vet Res 2017: 0.811

CiteScore 2017: 0.68

SCImago Journal Rank (SJR) 2017: 0.29
Source Normalized Impact per Paper (SNIP) 2017: 0.484


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