Fusarium Oxysporum F. Sp. Radicis-Lycopersici – the Cause of Fusarium Crown and Root Rot in Tomato Cultivation

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Fusarium oxysporum f. sp. radicis-lycopersici (FORL) leading to fusarium crown and root rot is one of the most destructive soilborne diseases of tomatoes occurring in greenhouse and field crops. Physiological races of FORL were not defined but nine vegetative compatibility groups (VGCs) were identified. Infection followed by wounds and natural holes and infection is not systemic. The optimum soil temperature for pathogen development is 18°C. Infection may cause plants to wilt and die completely or infection may lower fruit quality. Fusarium oxysporum f. sp. radicis-lycopersici has the ability to produce a specific enzyme, tomatinase, which breaks down α-tomatine and protects the pathogen. In contrast tomato also has a defence system which consists of the enzymes chitinase and β-1, 3-glucanase. Tomato resistance to Fusarium oxysporum f. sp. radicis-lycopersici is determined by a single dominant gene Frl, localized on the long arm of chromosome 9. It was introduced to cultivars from Licopersicum peruvianum (L.) Mill.

Armstrong G.M., Armstrong J.K. 1981. Formae speciales and races of Fusarium oxysporum causing wilt disease. p. 392-399. In: “Fusarium: Disease, Biology and Taxonomy” (P.E. Nelson, T.A. Toussoun, R.J. Cook, eds.). University Park: The Pennsylvania State University, 457 pp.

Balmas V., Scherm B., Di Primo P., Rau D., Marcello A., Migheli Q. 2005. Molecular characterization of vegetative compatibility group in Fusarium oxysporum f. sp. radicis-lycopersici and f. sp. lycopersici by random amplification of polymorphic DNA and microsatellite-primed PCR. Eur. J. Plant Pathol. 111 (1): 1-8.

Benhamou N., Charest P.M., Jarvis W.R. 1989. Biology and host parasite relations of Fusarium oxysporum f. sp. radicis-lycopersici. p. 95-105. In: “Vascular Wilt Disease of Plants: Basic Studies and Control” (E.C. Tjamos, C.H. Beckman, eds.). NATO ASI Ser Ser H Cell Biol Vol H28 Berlin, 604 pp.

Benhamou N., Joosten M.H.A.J., De Wit P.J.G.M. 1990. Subcellular localization of chitinase and of its potential substrate in tomato root tissues infected by Fusarium oxysporum f. sp. radicis-lycopersici. Plant Physiol. 92 (4): 1108-1120.

Benhamou N., Lafontaine P.J., Nicole M. 1994. Induction of systemic resistance to fusarium crown and root rot in tomato plants by seed treatment with chitosan. Phytopathology 84: 1432-1444.

Berry S.Z., Oakes G.L. 1987. Inheritance of resistance to fusarium crown and root rot in tomato. HortScience 22 (1): 110-111.

Brayford D. 1996. IMI descriptions of fungi and bacteria set 127. Mycopathologia 133 (1): 61-63.

Caron M., Fortin J.A., Richard C. 1986. Effect of phosphorus concentration and Glomus intraradices on fusarium crown and root rot of tomato. Phytopathology 76: 942-946.

Cook R.J., Baker K.F. 1983. The Nature and Practice of Biological Control and Plant Pathogens. APS Press, St. Paul, MN, 539 pp.

Datnoff L.E., Nemec S., Pernezny K. 1995. Biological control of fusarium crown and root rot of tomato in Florida Rusing Trichoderma harzianum and Glomus intraradices. Biol. Control 5 (3): 427-431.

de las Heras A. Patino B., Posada M.L., Martines M.J., Vazques C., Gonzales Jaen M.T. 2003. Characterization and in vitro expression patterns of an exopolygalacturonase encoding gene from Fusarium oxysporum f.sp. radicis lycopersici. J. Appl. Microbiol. 94 (5): 856-864.

Di Primo P., Cartia G., Katan T. 2001. Vegetative compatibility and heterokaryon stability in Fusarium oxysporum f. sp. radicis-lycopersici from Italy. Plant Pathol. 50 (3): 371-382.

Duffy B.K., Defago G. 1997. Zinc improves biocontrol of fusarium crown and root rot of tomato by Pseudomonas fluorescens and represses the production of pathogene metabolites inhibitory to bacterial antibiotic biosynthesis. Phytopathology 87: 1250-1257.

Elkind Y., Kedar N., Katan Y., Couteaudier Y., Laterrot H. 1988. Linkage between Tm-2 and Fusarium oxysporum f. sp. radicis-lycopersici resistance (FORL). Rep. Tomato Genet. Coop. 38, p. 22.

Fazio G., Stevens M.R., Scott J.W. 1999. Identification of RAPD markers linked to fusarium crown and root rot resistance (Frl) in tomato. Euphytica 105 (3): 205-210.

Hartman J.R., Fletcher J.T. 1991. Fusarium crown and root rot of tomatoes in the UK. Plant Pathol. 40 (1): 85-92.

Ito S., Nagata A., Kai T., Takahara H., Tanaka S. 2005. Symptomless infection of tomato plants by tomatinase producing Fusarium oxysporum formae speciales nonpathogenic on tomato plants. Physiol. Mol. Plant Pathol. 66 (5): 183-191.

Jarvis W.R., Thorpe H.J. 1976. Susceptibility of Lycopersicon species and hybrid to the foot and root rot pathogen Fusariumoxysporum. Plant Dis. Rep. 60 (12): 1076-1031.

Jarvis W.R., Shoemaker R.A. 1978. Taxonomic status of Fusariumoxysporum causing foot and root rot of tomato. Phytopathology 68: 1679-1680.

Jones J.B, Jones J.P., Stall R.E., Zitter T.A. 1991. Compendium of Tomato Diseases. APS, St. Paul, MN, USA, 100 pp.

Kamilova F., Kravchenko L.V., Shaposhinkova A.I., Makarova N., Lugtenberg B. 2006. Effect of tomato pathogen Fusariumoxysporum f.sp. radicis-lycopersici and of the biocontrol bacterium Pseudomonas fluorescens WCS365 on the composition of organic acids and sugars in tomato root exudates. Mol. Plant Microbe Interact. 19 (10): 1121-1126.

Katan T., Zamir D., Sarfatti M., Katan J. 1991. Vegetative compatibility groups and subgroups in Fusarium oxysporum f. sp. radicis-lycopersici. Phytopathology 81: 255-262.

Katan T., Katan J. 1999. Vegetative compatibility in Fusariumoxysporum f.sp. radicis-lycopersici from the UK, the Netherlands, Belgium and France. Plant Pathol. 48 (4): 541-9.

Kim J.T., Park I.H, Oung I.H., Yu S.H. 2001. Crown and root rot of greenhouse tomato caused by Fusarium oxysporum f. sp. radicis-lycopersici in Korea. Plant Pathol. J. 17 (5): 290-294.

Lagopodi A.L., Ram A.F.J., Lamers G.E.M., Punt P.J., Van den Hondel C.A.M.J.J., Lugtenberg B.J.J, Bloemberg G.V. 2002. Novel aspects if tomato root colonization and infection by Fusarium oxysporum f. sp. radicis-lycopersici revealed by confocal laser scanning microscopic analysis using the green fluorescent protein as a marker. Mol. Plant Microbe Interact. 15 (2): 172-179.

Lairini K., Perez-Espinosa A., Pineda M., Ruiz-Rubio M. 1996. Purification and characterization of tomatinase from Fusariumoxysporum f. sp. radicis-lycopersici. Appl. Environ. Microbiol. 62 (5): 1604-1609.

Louter J.H., Edgington L.V. 1990. Indications of cross-protection against fusarium crown and root rot of tomato. Can. J. Plant Pathol. 12 (3): 283-288.

Mauch F., Mauch-Mani B., Boller T. 1988. Antifungal hydrolases in pea tissue. II. Inhibition of fungal growth by combinations of chitinase and β-1, 3-glucanase. Plant Physiol. 88 (3): 936-942.

Menzies J.G., Ehret D.L. 1997. Root fungi increase the growth and yield and decrease the severity of fusarium crown and root rot of tomato plants grown in soilless culture (refered). International Symposium Growing Media and Plant Nutrition in Horticulture. ActaHort. 450: 457-466.

Mihuta-Grimm L., Erb W.A., Rowe R.C. 1990. Fusarium crown and root rot of tomato in greenhouse rock wool systems: sources of inoculum and disease management with benomyl. Plant Dis. 74 (12): 996-1002.

Omar I., O’Neill T.M., Rossall S. 2006. Biological control of fusarium crown and root rot of tomato with antagonistic bacteria and integrated control when combined within the fungicide carbendazim. Plant Pathol. 55 (1): 92-99.

Rekah, Y., Shtienberg, D., Katan, J. 1999. Spatial distribution and temporal development of fusarium crown and root-rot of tomato and pathogen dissemination in field soil. Phytopathology 89: 831-839.

Rekah Y., Shtienberg D., Katan J. 2000. Disease development following infection of tomato and basil foliage by airborne conidia of the soilborne pathogens Fusarium oxysporum f. sp. radicis-lycopersici and F. oxysporum f. sp. basilica. Phytopathology 90: 1322-1329.

Roddick J.G. 1974. The steroidal glycoalkaloid α-tomatine. Phytochemistry 13 (1): 9-25.

Roddick J.G., Drysdale R.B. 1984. Destabilization of liposomy membranes by the steroidal glycoalkaloid α-tomatine. Phytochemistry 23: 543-547.

Rowe R.C., Farley J.D. 1981. Strategies for controlling fusarium crown and root rot in greenhouse tomatoes. Plant Dis. 65 (2): 107-112.

Sato R., Araki T. 1974. On the tomato root-rot disease occurring under vinyl-house conditions in southern Hokkaido. Ann. Rep. Soc. Plant Prot. North Jap. 25: 5-13.

Sivan A., Chet I. 1993. Integrated control of fusarium crown and root rot of tomato with Trichoderma harzianum in combination with methyl bromide or soil solarization. Crop Prot. 12 (5): 380-386.

Sonoda R.M. 1976. The occurrence of fusarium root rot of tomatoes in Florida. Plant Dis. Rep. 60: 271-274.

Steinkellner S., Mammerler R., Vierheilig H. 2005. Microconidia germination of the tomato pathogen Fusarium oxysporum in the presence of root exudates. J. Plant Interac. 1 (1): 23-30.

Ślusarski Cz. 2000. Choroby odglebowe pomidorów (cz. II). W bezglebowej uprawie pod oslonami. Haslo Ogrodnicze 2: 46-49.

van Os E.A. 1999. Closed soilless growing system: a sustainable solution for Dutch greenhouse horticulture. Water Sci. Technol. 39 (5): 105-112.

Vakalounakis D.J. 1988. The genetic analysis of resistance to fusarium crown and root rot of tomato. Plant Pathol. 37 (1): 71-73.

Vakalounakis D.J., Laterrot H., Moretti A., Ligoxigakis E.K., Smardas K. 1997. Linkage between Frl (Fusarium oxysporum f. sp. radicis-lycopersici resistance) and Tm-2 (tobacco mosaic virus resistance-2) loci in tomato (Lycopersicon esculentum). Ann. Appl. Biol. 130 (2): 319-323.

Yamamoto I., Konad H., Kuniyasu M., Saito M., Ezuka A. 1974. A new race of Fusarium oxysporum f. sp. lycopersici including root rot of tomato. Proc. Kansai Plant Proc. Soc. 16: 17-29.

Journal of Plant Protection Research

The Journal of Polish Society of Plant Protection, Committee of Plant Protection; Polish Academy of Sciences, Institute of Plant Protection – National Research Institute

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