Post-Effort Changes in Activity of Traditional Diagnostic Enzymatic Markers in Football Players’ Blood / Promene U Aktivnostima Tradicionalnih Dijagnostičkih Enzimskih Markera U Krvi Fudbalera Posle Fizičkog Naprezanja

Open access

Summary

Background: Long-term and intensive physical effort causes metabolic and biochemical adaptations for both athletic and non-athletic objectives. Knowing the importance of aerobic training in football players, the aim of this study was to evaluate changes in the activity of: creatinine kinase (CK), creatine kinase MB (CKMB), lactate dehydrogenase (LDH), ahydroxybutyrate dehydrogenase (HBDH), cholinesterase (ChE) and alkaline phosphatase (ALP) in response to a semi-long distance outdoor run under aerobic conditions among both female and male football players.

Methods: Sixteen participants aged 21.9±2 years (women) and 18.4±0.5 years (men), all of them voluntarily recruited football players, took part in an outdoor run, the women covering a distance of 7.4±0.3 km while men covered a distance of 10.7±1.0 km. Plasma activities of the studied enzymes were determined using an appropriate diagnostic assay kit.

Results: Our results indicate that total LDH activity could be a useful tool in evaluating physical fitness among athletes. We simultaneously established that ChE could not be a marker useful in assessing metabolic response to physical effort in athletes. Moreover, our results suggest that post-effort changes in ALP activity might be used to estimate early symptoms of certain vitamin deficiencies in an athlete’s diet.

Conclusions: We confirmed that the assessment of activity of selected traditional diagnostic enzymatic markers provides information about muscle state after physical effort.

1. Banfi G, Colombini A, Lombardi G, Lubkowska A. Metabolic markers in sports medicine. Adv Clin Chem 2012; 56: 1-54.

2. Fallon KE. The clinical utility of screening of biochemical parameters in elite athletes: analysis of 100 cases. Br J Sports Med 2008; 42: 334-7.

3. Cazzola R, Russo-Volpe S, Cervato G, Cestaro B. Biochemical assessments of oxidative stress, erythrocyte membrane fluidity and antioxidant status in professional football players and sedentary controls. Eur J Clin Invest 2003; 33: 924-30.

4. Gravina L, Ruiz F, Lekue JA, Irazusta J, Gil SM. Metabolic impact of a soccer match on female players. J Sports Sci 2011; 29: 1345-52.

5. Cadefau J, Casademont J, Grau JM, Fernandez J, Balaguer A, Vernet M, et al. Biochemical and histochemical adaptation to sprint training in young athletes. Acta Physiol Scand 1990; 140: 341-51.

6. Meyer T, Meister S. Routine blood parameters in elite soccer players. Int J Sports Med 2011; 32: 875-81.

7. Wiacek M, Andrzejewski M, Chmura J, Zubrzycki IZ. The changes of the specific physiological parameters in response to 12-week individualized training of young soccer players. J Strength Cond Res 2011; 25: 1514-21.

8. Mougios V. Reference intervals for plasma creatine kinase in athletes. Br J Sports Med 2007; 41: 674-8.

9. Sherwi N, Pellicori P, Joseph AC, Buga L. Old and newer biomarkers in heart failure: from pathophysiology to clinical significance. J Cardiovasc Med (Hagerstown) 2013; 14: 690-7.

10. Buryta R. Significance of controlling the intensity of various types of effort in football. Centr Eur J Sport Sci Med 2013; 2: 3-8.

11. Hoff J. Training and testing physical capacities for elite soccer players. J Sports Sci 2005; 23: 573-82.

12. Popadic Gacesa JZ, Barak OF, Grujic NG. Maximal anaerobic power test in athletes of different sport disciplines. J Strength Cond Res 2009; 23: 751-5.

13. Manna I, Khanna GL, Chandra Dhara P. Effect of training on physiological and biochemical variables of soccer players of different age groups. Asian J Sports Med 2010; 1: 5-22.

14. Kruk J. Good scientific practice and ethical principles in scientific research and higher education. Centr Eur J Sport Sci Med 2013; 1: 25-9.

15. Majkić-Singh N, [umarac Z. Quality indicators of the pre-analytical phase. J Med Biochem 2012; 31: 174-83.

16. Castagna C, Abt G, D’Ottavio S. Physiological aspects of soccer refereeing performance and training. Sports Med 2007; 37: 625-46.

17. Brancaccio P, Maffulli N, Limongelli FM. Creatine kinase monitoring in sport medicine. Br Med Bull 2007; 81-82: 209-30.

18. Vincent HK, Vincent KR. The effect of training status on the plasma creatine kinase response, soreness and muscle function following resistance exercise. Int J Sports Med 1997; 18: 431-7.

19. Brancaccio P, Limongelli FM, Maffulli N. Monitoring of serum enzymes in sport. Br J Sports Med 2006; 40: 96-7.

20. Lucia A, Moran M, Perez M, Saborido A, Diaz E, Megias A, et al. Short-term effects of marathon running in master runners: no evidence of myocardial injury. Int J Sports Med 1999; 20: 482-6.

21. Diaz E, Ruiz F, Hoyos I, Zubero J, Gravina L, Gil J, et al. Cell damage, antioxidant status, and cortisol levels related to nutrition in ski mountaineering during a two-day race. J Sports Sci Med 2010; 9: 338-46.

22. Lippi G, Salvagno GL, Montagnana M, Schena F, Ballestrieri F, Guidi GC. Influence of physical exercise and relationship with biochemical variables of NT-pro-brain natriuretic peptide and ischemia modified albumin. Clin Chim Acta 2006; 367: 175-80.

23. Lippi G, Schena F, Montagnana M, Salvagno GL, Banfi G, Guidi GC. Significant variation of traditional markers of liver injury after a half-marathon run. Eur J Intern Med 2011; 22: e36-38.

24. Klapcińska B, Iskra J, Poprzecki S, Grzesiok K. The effects of sprint (300 m) running on plasma lactate, uric acid, creatine kinase and lactate dehydrogenase in competitive hurdlers and untrained men. J Sports Med Phys Fitness 2001; 41: 306-11.

25. Ohkuwa T, Saito M, Miyamura M. Plasma LDH and CK activities after 400 m sprinting by well-trained sprint runners. Eur J Appl Physiol Occup Physiol 1984; 52: 296-9.

26. Favero TG, Stavrianeas S, Klug GA. Training-induced alterations in lactate dehydrogenase reaction kinetics in rats: a re-examination. Exp Physiol 1999; 84: 989-98.

27. Mena P, Maynar M, Campillo JE. Changes in plasma enzyme activities in professional racing cyclists. Br J Sports Med 1996; 30: 122-4.

28. Brown SS, Kalow W, Pilz W, Whittaker M, Woronick CL. The plasma cholinesterases: a new perspective. Adv Clin Chem 1981; 22: 1-123.

29. Turecky L, Kupcova V, Mojto V, Smutny M, Uhlikova E, Vozar I. Serum cholinesterase activity and proteosynthetic function of liver in patients with diabetes mellitus. Bratisl Lek Listy 2005; 106: 266-9.

30. Iida T, Harada T, Ishizaki F, Nitta Y, Aoi S, Ikeda H, et al. Changes in bone mineral density and metabolism in women: evaluation of bodily characteristics, bone metabolic markers and bone mineral density. Hiroshima J Med Sci 2013; 62: 49-53.

31. Bayhan I, Dogan NU, Ozaksit G, Uygur D, Ugurlu N, Ugur M. Effect of strontium ranelate on serum leptin and bone turnover markers in women with established postmenopausal osteoporosis. J Reprod Med 2013; 58: 319-23.

Journal of Medical Biochemistry

The Journal of Society of Medical Biochemists of Serbia

Journal Information


IMPACT FACTOR 2017: 1.378
5-year IMPACT FACTOR: 0.704



CiteScore 2017: 1.05

SCImago Journal Rank (SJR) 2017: 0.307
Source Normalized Impact per Paper (SNIP) 2017: 0.532

Cited By

Metrics

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 172 171 13
PDF Downloads 58 58 4