Varroacidal Efficiency of Treatment with Amitraz in Honey Bee Colonies with Brood

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Field trials were conducted to evaluate the effectiveness of amitraz fumigation against Varroa destructor in honey bee colonies with brood. Within this project the following aspects were taken into consideration: strength of colony, the number of treatments, time intervals between treatments and way of its performance. Honey bee colonies with brood were fumigated four times with one tablet of Apiwarol® per each treatment every four, six, eight and ten days. The tablets with amitraz were burned in the electrical device Wakont or directly in hives. In case of amitraz fumigation with Wakont even four treatments reduced infestations of V. destructor to a limited extent, on average from 40 to 61% of mite populations. A similar effectiveness among the treatments has been ascertained regardless of intervals between them. The efficacy of amitraz combustion in hives was slightly higher and statistically significant only after four treatments. Moreover, beyond brood area, the population of worker bees turned out to determine treatment efficacy with amitraz in this form and modes of administration. In honey bee colonies with brood, even four amitraz fumigations do not decrease the level V. destructor infestation to the extent that it is safe for wintered bees.

Bąk, B., Wilde, J., & Siuda, M. (2013). Efficiency of Varroa destructor management with medications used in Poland. Medycyna Weterynaryjna, 69(12), 744-748.

Dai, P., Jack, C.J., Mortensen, A.N., Bustamante, T.A., Ellis, J.D. (2018). Chronic toxicity of amitraz, coumaphos and fluvalinate to Apis mellifera L. larvae reared in vitro. Scientific Reports, 8, 5635.

Gregorc, G.A., & Bowen, I.D. (2000). Histochemical characterization of cell death in honey bee larvae midgut after treatment with Paenibacillus larvae, amitraz, and oxytetracycline. Cell Biology International, 24(5), 319-324. DOI: 10.1006/cbir.1999.0490

Gregorc, G.A., & Ellis, J.D. (2011). Cell death localization in situ in laboratory reared honey bee (Apis mellifera L.) larvae treated with pesticides. Pesticide Biochemistry and Physiology, 99(2), 200-207.

Kamler, M., Nesvorna, M., Stara, J., Erban, T., Hubert, J. (2016). Comparison of tau-fluvalinate, acrinathrin, and amitraz effects on susceptible and resistant populations of Varroa destructor in a vial test. Experimental and Applied Acarology, 69(1), 1-9. DOI: 10.1007/s10493-016-0023-8

Kiljanek, T., Niewiadowska, A., Gaweł, M., Semeniuk, S., Borzęcka, M., Posyniak, A., Pohorecka, K. (2017). Multiple pesticide residues in live and poisoned honeybees - Preliminary exposure assessment. Chemosphere, 175, 36-44. DOI: 10.1016/j.chemosphere.2017.02.028

Loucif-Ayad, W., Aribi, N., Smagghe, G., & Soltani, N. (2010). A scientific note on the impact of acaracides on the nutritional biochemistry of Apis mellifera intermissa (Hymenoptera: Apidae) Apidologie, 41(2), 135-137. DOI: 10.1051/apido/2009063

Maggi, M.D., Ruffinengo, S.R., Negri, P., & Eguaras, M.J. (2010). Resistance phenomena to amitraz from populations of the ectoparasitic mite Varroa destructor of Argentina. Parasitology Research, 107(5), 1189.

O’Neal, S.T., Brewster, C.C., Bloomquist, J.R., & Anderson, T.D. (2017). Amitraz and its metabolite modulate honey bee cardiac function and tolerance to viral infection. Journal of Invertebrate Pathology, 149, 119-126. DOI:10.1016/j.jip.2017.08.005

Pohorecka, K., & Bober, A. (2007). Oporność V. destructor na najczęściej stosowane akarycydy. Medycyna Weterynaryjna, 63(8), 904-908.

Pohorecka, K., & Bober, A. (2008). Porównanie wrażliwości na amitraz populacji Varroa destructor pochodzących z pasiek leczonych amitrazem i fluwalinatem. In Materiały z XLV Naukowa Konferencja Pszczelarska. Puławy, 11-12 marca, 2008, 83-85.

Pohorecka, K., Bober, A., Skubida, M., Zdańska, D., Torój, K. (2014). A comparative study of environmental conditions, bee management and the epidemiological situation in apiaries varying in the level of colony losses. Journal of Apicultural Science, 58(2), 107-132.

Pohorecka, K., Kiljanek, T., Antczak, M., Skubida, P., Semkiw, P., Posyniak, A. (2018). Amitraz marker residues in honey from honeybee colonies treated with Apiwarol. Journal of Veterinary Research, 62(3), 297-301.

Pohorecka, K., Skubida, P., Semkiw, P., Miszczak, A., Sikorski, P., Zagibajło, … Bober, A. (2013). Effects of exposure of honey bee colonies to neonicotinoid seed-treated maize crops. Journal of Apicultural Science, 57(2), 199-208.

Pohorecka, K., Szczęsna, T., Witek, M., Miszczak, A., Sikorski, P. (2017). The exposure of honey bees to pesticide residues in the hive environment with regard to winter colony losses. Journal of Apicultural Science, 61(1), 105-125.

Rosenkranz, P., Aumeier, P., & Ziegelmann, B. (2010). Biology and control of Varroa destructor. Journal of Invertebrate Pathology, 103, Supplement, 96-119. DOI: 10.1016/j.jip.2009.07.016

Semkiw, P., Skubida, P., & Pohorecka, K. (2013). The amitraz strips efficacy in control of Varroa destructor after many years application of amitraz in apiaries. Journal of Apicultural Science, 57(1), 107-121.

Strachecka, A., Paleolog, J., Olszewski, K., & Borsuk, G. (2012). Influence of amitraz and oxalic acid on the cuticle proteolytic system of Apis mellifera L. workers. Insects, 3(3), 821-832.

Węgrzynowicz, P., Białek, T., Gerula, D., Panasiuk, B., Bieńkowska, M., Skwarek, E. (2017). Skuteczność zwalczania pasożytów Varroa destructor preparatem Biowar 500. In Materiały z 54 Naukowa Konferencja Pszczelarska. Puławy, 7-8 marca 2017, 46.

Journal of Apicultural Science

The Journal of Research Institute of Horticulture and Apicultural Research Association

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