Microbiological Contamination of Honeys from Different Sources in Turkey

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Honey samples obtained by beekeepers taken from thirty-five different apiaries in Turkey’s region of Marmara were investigated for the presence of microorganism. Each honey sample was examined for the number of total aerobic mesofilic bacteria, coliforms, moulds and yeasts and the presence of Salmonella spp., Clostridium botulinum, along with other aerobic bacteriae such as Paenibacillus larvae and Melissococcus plutonius. In total, fifty-four honey samples of different botanical origins including unifloral (Umbelliferae, Leguminosae, Trifolium, Onobrychis), multifloral and chestnut were evaluated in the means of microbiological properties. Microorganisms were isolated in twenty-eight samples (60.86%) of pure cultures and eighteen samples (39.13%) of mixed cultures. On the other hand, no microorganisms were isolated in eight samples. Bacillus spp, Corynebacterium spp., Streptococcus spp., Staphylococcus spp. C. albicans and Penicillium spp. were isolated and identified in other honey samples. The bacteria counts were 4×102 - 1.4×103 cfu/g for aerobic mesofilic bacteria and 1-185 cfu/g for the fungi. The application of sanitary practices (hand washing, avoidance of sneezing or coughing, etc.) may be effective in controlling contamination by microorganisms. On the other hand, air, equipment and dust may be contributing causes of microbiological contamination. Therefore it is important to take precautions in order to avoid such contamination, even though such factors are often difficult to control.

Delmas, C., Vidon, D.J.M., & Sebald, M. (1994). Survey of honey for Clostridium botulinum spores in Eastern France. Food Microbiology, 11, 515-518. DOI: https://doi.org/10.1006/fmic.1994.1058

Finola, M.S., Lasagno, M.C., & Marioli, J.M. (2007). Microbiological and chemical characterization of honeys from central Argentina. Food Chemistry, 100, 1649-1653. DOI: https://doi.org/10.1016/j.foodchem.2005.12.046

Gilmore, D., Link, L., & Fell, R. (2010). Analysis of bacterial pathogens in Virginia honeys. Science of Bee Culture; 2, 11-14.

Gunes, M.E., Sahin, S., Demir, C. Borum, E., Tosunoğlu, A. (2017). Determination of phenolic compounds profile in chestnut and floral honeys and their antioxidant and antimicrobial activities. Journal of Food Biochemistry, 41(3), e12345. DOI: https://doi.org/10.1111/jfbc.12345

Iurlina, M.O., & Fritz, R. (2005). Characterization of microorganisms in Argentinean honeys from different sources. International Journal of Food Microbiology, 105, 297-304. DOI: https://doi.org/10.1016/j.ijfoodmicro.2005.03.017

Joseph, T., Julius, A.N., Florence, F., Delphine, D.N., Jonnas, P., Antoine, M.Z. (2007). Physico-chemical and microbiological characteristics of honey from the sudano-guinean zone of West Cameroon. African Journal of Biotechnology, 6(7), 908-913.

Kacaniov, M., Melich, M., Knazovicka, V., Hascik, P., Sudzinova, J., Pavlicova, S., Cubon, J. (2009). The indicator microorganisms value in relation to primary contamination of honey. Zootechnie şi Biotecnologii, 42(2), 159-166.

Küplülü, Ö., Göncüoğlu, M., Özdemir, H. & Koluman, A. (2006). Incidence of Clostridium botulinum spores in honey in Turkey. Food Control, 17, 222-224. DOI: https://doi.org/10.1016/j.foodcont.2004.10.004

Louveaux, J., Maurizio, A., & Vorwohl, G. (1978). Methods of melissopalynology. Bee World, 59, 139–157.

Marshal, L., Bourdon, J.P., & Richard, C. (1987). Les milieux de culture pour I’solement et I’dentification de bacteries. (pp. 230-232) Paris: Dion editeur.

Nakano, H., & Sakaguchi, G. (1991). An unusually heavy contamination of honey products by Clostridium botulinum type F and Bacillus alvei. FEMS Microbiology Letters, 79, 171-178.

Omafuvbe, B.O., & Akanbi, O.O. (2009). Microbiological and physico-chemical properties of some commercial Nigerian honey. African Journal of Microbiology Research, 3(12), 891-896.

Piana, M.L., Poda, G., Cesaroni, D., Cuetti, L., Bucci, M.A., Gotti, P. (1991). Research on microbial characteristics of honey samples of Udine province. Rivista della Societa Italiana Scienze dell Alimentazione, 20, 293-301.

Sadık, M.W., & Ali, M.A.M. (2012). Survey and identification of microorganisms in bee honey samples collected from different plant sources and regions in Saudi Arabia. Global Advanced Research Journals, 1(8), 126-134.

Sinacori, M., Francesca, N., Alfonzo, A., Cruciata, M., Sannino, C., Setanni, L., Moschetti, G. (2014). Cultivable microorganisms associated with honeys of different geographical and botanical origin. Food Microbiology, 38, 284-294. DOI: https://doi.org/10.1016/j.fm.2013.07.013

Snowdon, J.A., & Cliver, D.O. (1996). Microorganisms in honey. International Journal of Food Microbiology, 31, 1-26. DOI: https://doi.org/10.1016/0168-1605(96)00970-1

Tudor, L., Mitranescu, E., Galiş, A.M., Illie, L.I., Ceauşi, C. (2011). Microbiological and physicochemical analysis of honey from southern Romania. Proceedings of XVth International Congress of the International Society for Animal Hygiene (p 1007-1011). Vienna, Austria.

Tysset, C., Durand, C., & Taliergio, Y.P. (1970). Contribution to the study of the microbial contamination and the hygiene of commercial honey. Revue de Medecine Veterinaire, 146, 1471-1492.

Tysett, C., & Rosseau, M. (1981). Problem of microbes and hygiene of commercial honey. Revue de Medecine Veterinaire, 132, 591-600.

Vica, M., Glevitzky, M., Dumitrel, G.A., Popa, M., Varvara, S. (2009). Microbiological role in hazards analysis of natural honey processing. Journal of Agroalimentary Processes Technologies, 15(3), 353-360.

White, J.W., Subers, M.H., & Schepartz, A.I. (1963). The identification of inhibine, the antibacterial factor in honey, as hydrogen peroxide and its origin in a honey glucose-oxidase system. Biochimica Biophysica Acta, 73, 57-70.

Journal of Apicultural Science

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