The Spring Assessment of Nosema Spp. Infection in Honey Bee Colonies (Apis mellifera L.) - Sampling as an Important Aspect of a Reliable Diagnosis

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The objective of the research was a comparative assessment of the infection levels of Nosema spp. in honey bees collected from different areas of the hive. A total of 588 honey bee colonies were sampled in spring (April-May) 2015 and 2016 through the simultaneous collection of dead worker bees from the bottom board of the hive and live bees from peripheral combs. A microscopic assay of composite samples of 60 bees was used for the detection and quantification of Nosema spp. spores. Consistent positive results of laboratory tests (detection of Nosema spp. spores in both live and dead bee samples) were achieved for 28% of colonies from surveyed group. In 36% of colonies both types of samples were Nosema-negative. Spores of Nosema spp. were detected solely in worker bees from the bottom board or exclusively in bees from nests in every 18% of sampled colonies. No differences were found between the share of colonies that had been identified as Nosema-infected on the basis of an analysis of only the live or dead bees (46% versus 46%). Laboratory examination of both types of bee samples can improve the reliability and accuracy of spore counting for the diagnosis of Nosema spp. infection in spring. The introduction of this sampling strategy in routine laboratory diagnostics can be considered as an alternative to the application of more sensitive PCR methods or sampling a higher number of live bees.

Bailey, L. (1981). Honey Bee Pathology. London, UK: Academic Press.

Botías, C., Martín-Hernández, R., Meana, A., & Higes, M. (2012). Critical aspects of the Nosema spp. diagnostic sampling in honey bee (Apis mellifera L.) colonies. Parasitology Research, 110(6), 2557-2561.

Borsuk, G., Olszewski, K., Strachecka, A., & Paleolog, J. (2011). The interaction of worker bees which have increased genotype variance. II. Cage tests of sugar syrup collecting and mortality. Journal of Apicultural Science, 55 (1), 59-65.

Doull, K.M., & Eckert, J.E. (1962). A survey of the incidence of Nosema disease in California. Journal of Economic Entomology, 55(3), 313-317.

Forsgren, E., & Fries, I. (2010). Comparative virulence of Nosema ceranae and Nosema apis in individual European honey bees. Veterinary Parasitology, 170(3-4), 212-217.

Fries, I. (1993). Nosema apis - a parasite in the honey bee colony. Bee World,74(1), 5-19.

Fries, I. (2010). Nosema ceranae in European honey bees (Apis mellifera). Journal of Invertebrate Pathology, 103, S73–S79.

Fries, I., Ekbohm, G., & Villumstad, E. (1984). Nosema apis, sampling techniques and honey yield. Journal of Apicultural Research, 23(2), 102-105.

Fries, I., Feng, F., Da Silva, A., Slemenda, S.B., Pieniazek, N.J. (1996). Nosema ceranae n. sp. (Microspora, Nosematidae), morphological and molecular characterization of a microsporidian parasite of the Asian honey bee Apis cerana (Hymenoptera, Apidae). European Journal of Protistology, 32, 356-365.

Gisder, S., Hedtke, K., Möckel, N., Frielitz, M.C., Linde, A., Genersch, E. (2010). Five-year cohort study of Nosema spp. in Germany: does climate shape virulence and assertiveness of Nosema ceranae? Applied and Environmental Microbiology, 76(9), 3032-3038. DOI: 10.1128/AEM.03097-09

Higes, M., Martín-Hernández, R., Botías, C., Bailón, E.G., González-Porto, A.V., Barrios, L., …. Meana, A. (2008). How natural infection by Nosema ceranae causes honeybee colony collapse. Environmental Microbiology, 10(10), 2659-2669. DOI: 10.1111/j.1462-2920.2008.01687.x

Klee, J., Besana, A.M., Genersch, E., Gisder, S., Nanetti, A., Tam, D.Q., …. Paxton, R.J. (2007). Widespread dispersal of the microsporidian Nosema ceranae, an emergent pathogen of the western honey bee, Apis mellifera. Journal of Invertebrate Pathology, 96(1), 1-10.

Manlik, O., Schmid-Hempel, R., & Schmid-Hempel P. (2017). Parasite infection of specific host genotypes relates to changes in prevalence in two natural populations of bumblebees. Infection Genetics and Evolution, 56, 125-132. DOI: 10.1016/j.meegid.2017.11.019

Meana, A., Martín-Hernández, R., & Higes, M. (2010). The reliability of spore counts to diagnose Nosema ceranae infections in honey bees. Journal of Apicultural Research, 49(2), 212-214.

Mulholland, G.E., Traver, B.E., Johnson, N.G., & Fell R.D. (2012). Individual variability of Nosema ceranae infections in Apis mellifera colonies. Insects, 3(4): 1143-1155. DOI: 10.3390/insects3041143

Paleolog, J. (2009). Behavioural characteristics of honey bee (Apis mellifera) colonies containing mix of workers of divergent behavioural traits. Animal Science Papers and Reports, 27 (3), 237-248.

Pickard, R.S., & El-Shemy, A.A.M. (1989). Seasonal variation in the infection of honeybee colonies with Nosema apis Zander. Journal of Apicultural Research, 28(2), 93-100.

Pohorecka, K., Bober, A., Skubida, M., Zdańska, D., Torój, K. (2014). A comparative study of environmental conditions, bee management and the epidemiological situation in apiaries varying in the level of colony losses. Journal of Apicultural Science, 58(2): 107-132.

Pohorecka, K., & Muszyńska, J. (1996). Comparison of different methods to measure the infestation level of wintering honeybee colonies by the spores of Nosema apis Z. Pszczelnicze Zeszyty Naukowe, 40, 129-139.

Topolska, G., & Hartwig, A. (2005). Diagnosis of Nosema apis infection by investigations of two kinds of samples: dead bees and live bees. Journal of Apicultural Science, 42(2), 75-79.

Traver, B.E., Williams, M.R., & Fell, R.D. (2012). Comparison of within hive sampling and seasonal activity of Nosema ceranae in honey bee colonies. Journal of Invertebrate Pathology, 109(2):187-193. DOI:10.1016/j.jip.2011.11.001

World Organisation for Animal Health (OIE). (2017). Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 2.2.4 Nosemosis of honey bees, 1-6. Available online:

Zander, E. (1909). Tierische Parasiten als Kranken-heitserreger bei der Biene. Münch. Bienen-Zeitung, 31, 196-204.

Journal of Apicultural Science

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