Mitochondrial DNA Characterization of High Royal Jellyproducing Honeybees (Hymenoptera: Apidae) in China

Open access

Abstract

China is the largest producer and exporter of royal jelly in the world. The high production of royal jelly in China is mainly attributed to a high royal jelly-producing lineage of honeybees (Apis mellifera) (HRJB). However, few studies have been conducted on the genetic characterization of HRJB. In this study, the mitochondrial DNA intergenic region between cytochrome oxidase I and II (COI-COII) and the mitochondrial NADH dehydrogenase subunit 2 sequences (ND2) were determined for 90 HRJB colonies, collected from the regions of China where HRJB originated, and 25 unimproved A. m. ligustica colonies from China. COI-COII sequence analysis revealed two mitotypes (C1 and C2d) in HRJB colonies and one mitotype (C1) in unimproved A. m. ligustica colonies. The main mitotype (C1) in HRJB accounted for 93% of the colonies. Based on ND2 sequences, four and two mitotypes were found in HRJB and unimproved A. m. ligustica colonies, respectively. Sequence alignment showed that nucleotides in three positions of the ND2 sequence were different between the main mitotype of HRJB and that of unimproved A. m. ligustica. Our study suggested that HRJB was bred from A. m. ligustica and possibly had genetic characteristics different from unimproved A. m. ligustica.

Alburaki, M., Bertrand, B., Legout, H., Moulin, S., Alburaki, A., Sheppard, W. S., Garnery, L. (2013). A fifth major genetic group among honeybees revealed in Syria. BMC Genetics, 14(117), 1-10. DOI: 10.1186/1471-2156-14-117

Arias, M. C., & Sheppard, W. S. (1996). Molecular phylogenetics of honey bee subspecies (Apis mellifera L.) inferred from mitochondrial DNA sequences. Molecular Phylogenetics and Evolution, 5(3), 557-566. DOI: 10.1006/mpev.1996.0050

(ASAC) Apicultural Science Association of China. (2015). China’s bee products market review of 2014 and prospect for 2015. ASAC: Beijing [in Chinese].

Cao, L. F., Zheng, H. Q., Pirk, C. W. W., Hu, F. L., Xu, Z. W. (2016). High royal jelly-producing honeybees (Apis mellifera ligustica) (Hymenoptera: Apidae) in China. Journal of Economic Entomology, 109(2), 510-514. DOI: 10.1093/jee/tow013

Chen, S. L., Li, J. K., Zhong, B. X., & Su, S. K. (2005). Microsatellite analysis of royal jelly producing traits of Italian honeybee (Apis mellifera liguatica). Acta Genetica Sinica, 32(10), 1037-1044.

(CNCAGR) China National Commission of Animal Genetic Resources. (2011). Animal genetic resources in China-Bees. Chinese Agricultural Press: Beijing [in Chinese].

Cornuet, J. M., Garnery, L., & Solignac, M. (1991). Putative origin and function of the intergenic region between COI and COII of Apis mellifera L. mitochondrial DNA. Genetics, 128(2), 393-403.

Coroian, C. O., Muñoz, I., Schlüns, E. A., Paniti-Teleky, O. R., Erler, S., Furdui, E. M., … Moritz, R. F. A. (2014). Climate rather than geography separates two European honeybee subspecies. Molecular Ecology, 23 (9), 2353-2361. DOI: 10.1111/mec.12731

Crozier, R. H., & Crozier, Y. C. (1993). The mitochondrial genome of the honeybee Apis mellifera - complete sequence and genome organization. Genetics, 133(1), 97-117.

Franck, P., Garnery, L., Solignac, M., & Cornuet, J. M. (2000a). Molecular confirmation of a fourth lineage in honeybees from the near east. Apidologie, 31(2), 167-180. DOI: 10.1051/apido: 2000114

Franck, P., Garnery, L., Celebrano, G., Solignac, M., Cornuet, J. M. (2000b). Hybrid origins of honeybees from Italy (Apis mellifera ligustica) and Sicily (A. m. sicula). Molecular Ecology, 9(7), 907-921. DOI: 10.1046/j.1365-294x.2000.00945.x

Franck, P., Garnery, L., Loiseau, A., Oldroyd, B. P., Hepburn, H. R., Solignac, M., Cornuet, J. M. (2001). Genetic diversity of the honeybee in Africa: microsatellite and mitochondrial data. Heredity, 86(4), 420-430. DOI: 10.1046/j.1365-2540.2001.00842.x

Librado, P., & Rozas, J. (2009). DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25(11), 1451-1452. DOI: 10.1093/bioinformatics/btp187

Magnus, R. M., Tripodi, A. D., & Szalanski, A. L. (2011). Mitochondrial DNA diversity of honey bees, Apis mellifera L. (Hymenoptera: Apidae) from queen breeders in the United States. Journal of Apicultural Science, 55(1), 37-46.

Meixner, M. D., Pinto, M. A., Bouga, M., Kryger, P., Ivanova, E., Fuchs, S. (2013). Standard methods for characterising subspecies and ecotypes of Apis mellifera. Journal of Apicultural Research, 52(4), 1-27. DOI: 10.3896/IBRA.1.52.4.05

Muñoz, I., Dall’Olio, R., Lodesani, M., & Rúa, P. D. L. (2009). Population genetic structure of coastal Croatian honeybees ( Apis mellifera carnica). Apidologie, 40(6), 617-626. https://DOI.org/10.1051/apido/2009041

Muñoz, I., Stevanovic, J., Stanimirovic, Z., & Rúa, P. D. L. (2012). Genetic variation of Apis mellifera from serbia inferred from mitochondrial analysis. Journal of Apicultural Science, 56(1), 59-69. https://DOI.org/10.2478/v10289-012-0007-9

Ramadan, M. F., & Al-Ghamdi, A. (2012). Bioactive compounds and health-promoting properties of royal jelly: A review. Journal of Functional Foods, 4(1), 39-52. DOI: 10.1016/j.jff.2011.12.007

Ruttner, F. (1988). Biogeography and taxonomy of honey bees. Springer Verlag Press: Berlin.

Sambrook, J., Fritsch, E. F., & Maniatis, T. (1989). Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press: New York.

Sun, L. X., Chen, Z. Y., Yuan, J. J., & Xie, J. J. (2004). Genetic variability of MDHII in four lines of Apis mellifera ligustica. Joural of Zhangzhou Teachers College (Nat. Sci.), 17(1), 54-59 [in Chinese].

Sušnik, S., Kozmus, P., Poklukar, J., & Megli, V. (2004). Molecular characterisation of indigenous Apis mellifera carnica in Slovenia. Apidologie, 35(6), 623-636. DOI: 10.1051/apido: 2004061

Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F., Higgins, D. G. (1997). The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25(24), 4876-4882. DOI: 10.1093/nar/25.24.4876

Yin, L., Ji, T., Chen, G. H., & Peng, W. J. (2011). Genetic characterization of three breeds of high royal jelly producing honeybee (Apis mellifera ligustica) in China. African Journal of Agricultural Research, 6(2), 331-337. DOI: 10.5897/AJAR10.850

Journal of Apicultural Science

The Journal of Research Institute of Horticulture and Apicultural Research Association

Journal Information


IMPACT FACTOR 2017: 0.75
5-year IMPACT FACTOR: 1.007

CiteScore 2017: 0.92

SCImago Journal Rank (SJR) 2017: 0.345
Source Normalized Impact per Paper (SNIP) 2017: 0.461

Metrics

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 249 249 24
PDF Downloads 85 85 13