The greater wax moth Galleria mellonella L. (Lepidoptera: Pyralidae), one of the pests of honey bee (Apis mellifera L.) colonies, has spread almost all over the world. Although the G. mellonella is often reported to infest weak honey bee colonies that are exposed to pesticides and diseases, it is also a threat for healthy colonies. Therefore, there is a fairly high probability of transmission of both microflora-specific bacteria and pathogen microorganisms, especially Nosema species, between these organisms (Moth and bees). The aim of this study was to investigate the presence of Nosema species in greater wax moth G. mellonella collected from apiaries as well as grown in laboratory conditions. Adults and late instar larva of wax moth were used for detecting Nosema apis and Nosema ceranae. Real-time PCR amplification studies were performed and specific ITS regions were targeted to distinguish Nosema species. Real-time PCR results showed that N. apis and N. ceranae were found in both phases of G. mellonella. This is the first study to confirm that N. apis and N. ceranae are present in greater wax moth collected from apiaries and grown at laboratories in Turkey.
Akyol, E., & Korkmaz, A. (2008). The effect of -5°C cold application to protect honeycombs against greater wax-moth Galleria mellonella damage. Uludag Bee Journal, 8(1), 26-29.
Bourgeois, A.L., Rinderer, T.E., Beaman, L.D., Danka, R.G. (2010). Genetic detection and quantification Nosema apis and N. ceranae in the honey bee. Journal of Invertebrate Pathology, 103(1), 53-58.
Cantwell, G.E. (1970). Standard methods for counting Nosema spores. American Bee Journal, 110, 222-223.
Chen, P.Y., Evans, J.D., Murphy, C., Gutell, R., Zuker, M., Gundensen-Rindal, D., Pettis, J.S. (2009). Morphological, molecular and phylogenetic characterization of Nosema ceranae, a microsporidian parasite isolated from the European honey bee, Apis mellifera. Journal of Eukaryotic Microbiology, 56(2), 142-147. DOI: 10.1111/j.1550-7408.2008.00374.x
Coleman, J.J., Muhammed, M., Kasperkovitz, P.V., Vyas, J.M., Mylonakis, E. (2011). Fusarium pathogenesis investigated using Galleria mellonella as a heterologous host. Fungal Biology, 115(12), 1279-1289. DOI: 10.1016/j.funbio.2011.09.005
Ellis, J.D., Graham, J.R., & Mortensen, A. (2013). Standard methods for wax moth research. Journal of Apicultural Research, 52(1), 1-17. DOI: http://dx.doi.org/10.3896/IBRA.188.8.131.52
van Engelsdorp, D., & Meixner, M.D. (2010). A historical review of managed honey bee populations in Europe and the United States and the factors that may affect them. Journal of Invertebrate Pathology, 103, 80-95. DOI: 10.1016/j.jip.2009.06.011
Fisher, F.M., & Sanborn, R.C. (1962). Observations on the Susceptibility of Some Insects to Nosema (Microsporidia: Sporozoa). The Journal of Parasitology, 48(6), 926-932. DOI: 10.2307/3275124
Fries, I., Feng, F., DaSilva, A., Slemenda, S.B., Pieniazek, N.J. (1996). Nosema ceranae n. sp. (Microspora, Nosematidae), morphological and molecular characterization of a microsporidian parasite of the Asian honey bee Apis cerana (Hymenoptera: Apidae). European Journal of Protistology, 32(3), 356-365.
Fries, I., Martin, R., Meana, A., García-Palencia, P., Higes, M. (2006). Natural infections of Nosema ceranae in European honey bees. Journal of Apicultural Research, 45(4), 230-233. DOI: 10.3896/IBRA.184.108.40.206
Fries, I. (2010). Nosema ceranae in European honey bees (Apis mellifera). Journal of Invertebrate Pathology, 103, 573-579. DOI: 10.1016/j.jip.2009.06.017
Harding, C.R., Schroeder, G.N., Collins, J.W., Frankel, G. (2013). Use of Galleria mellonella as a Model Organism to Study Legionella pneumophila Infection. Journal of Visualized Experiments, 81, 1-10.
Higes, M., Martín, R., Sanz, A., Alvarez, N., Sanz, A., Garcia, M.P., Meana, A. (2005). El síndrome de despoblamiento de las colmenas en Espaňa. Consideraciones sobre su origen. Vida Apícola, 133, 15-21.
Higes, M., Martin-Hernandez, H., Botias, C., Bailon, E.G., Gonzalez-Porto, A.V., Barrios, L… Meana, A. (2008). How natural infection by Nosema ceranae causes honeybee colony collapse. Environmental Microbiology, 10(10), 2659-2669. DOI: 10.1111/j.1462-2920.2008.01687.x
Higes, M., Martín-Hernández, R., & Meana, A. (2010). Nosema ceranae in Europe: an emergent type C Nosemosis. Apidology, 41(3), 375-392. DOI: https://doi.org/10.1051/apido/2010019
Hu, K., & Webster, J.M. (2000). Antibiotic production in relation to bacterial growth and nematode development in Photorhabdus-Heterorhabditis infected Galleria mellonella larvae. FEMS Microbiology Letters, 189(2), 219-23.
Kučera, M., & Weiser, J. (1975). Lactate dehydrogenase isoenzymes in the larvae of Barathra brassicae and Galleria mellonella during microsporidan infection. Journal of Invertebrate Pathology, 25(1), 109-114.
Kučera, M., & Weiser, J. (1985). Different course of proteolytic inhibitory activity and proteolytic activity in Galleria mellonella larvae infected by Nosema algerae and Vairimorpha heterosporum. Journal of Invertebrate Pathology, 45(1), 41-46.
Lipa, J.J. (1977). Microsporidian infections of Galleria mellonella (L.) (Lepidoptera, Galleriidae) with the description of a new species Nosema galleriae n.sp. Acta Protozoologica, 16(2), 141-150.
Martín-Hernández, R., Meana, A., Prieto, L., Martínez Salvador, A., Garrido-Bailón, E., Higes, M. (2007). Outcome of colonization of Apis mellifera by Nosema ceranae. Applied and Environmental Microbiology, 73(20), 6331-6338. DOI: 10.1128/AEM.00270-07
Spangler, H.G. (1985). Sound production and communication by the greater wax moth (Lepidoptera: Pyralidae). Annals of the Entomological Society of America, 78(1), 54-61.
Williams, J.L. (1997). Insects: Lepidoptera (moths). In R Morse; K Flottum (Eds). Honey bee pests, predators, and diseases (pp. 121-141). Ohio, USA: The AI Root Company.